A new process for the separation and purification of egg yolk antibodies

A three stage process was developed for the separation of an antibody (IgY) from industrially separated chicken egg yolk. This included aqueous extraction of the water soluble fraction (WSF) from the yolk by dilution with distilled water and pH adjustment, separation of IgY from the WSF using a cation exchange column in an automated chromatography system, and finally purification of IgY using precipitation with sodium sulphate. The overall recovery of the process was approximately 50%, and the purity 95% or greater depending on the number of precipitation steps used. An automated liquid chromatography system was developed to allow efficient study of various chromatographic media for the separation of the antibody. Analysis of breakthrough curves indicates that superficial velocity is the governing parameter in the binding of IgY to the cation exchanger. Results of a pilot scale experiment involving the application of 46.5 litres of WSF containing approximately 0.8 mg/ml IgY to a 1500 nil column are presented.

Name thesis:degree_name

Doctor of Philosophy - PhD

Level thesis:degree_level

doctoral

Discipline thesis:degree_discipline

Chemical and Biological Engineering

Grantor dc:publisher

University of British Columbia

Year dc:date

1993

Author dc:creator

• Charter, Edward

Statement dc:rights

• For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.

Language dc:language

eng

Handle dc:identifier

http://hdl.handle.net/2429/1917

OAI identifier oai:identifier

oai:circle.library.ubc.ca:2429/1917