Silica based immunoassays for a covalently attached antigen

An immunoassay was developed using a monoclonal antibody and anantigen covalently immobilized on silica, showing that silica is a potentially useful assay substrate. Silica beads prepared from tetraethyl orthosilicate (0.78 pmdiameter) and fused quartz slides were thoroughly cleaned and modified with 3-aminopropyltriethoxysilane. Amine groups on the modified slides and beads were used for carbodiimide mediated coupling to carboxyl groups on a peptide antigen. High surface concentrations of antigen were achieved. Antibody binding to the antigen-modified beads and slides was measured using an iodinated monoclonal antibody. The equilibrium constant for the antibody binding on the beads was ten times higher than on the flat silica, but the maximum amount bound was lower. Immunoassays performed using a second enzyme-labelled antibody to detect the monoclonal antibody bound to the silica permitted detection of the antigen at 10-8 M using the modified beads and at10-7 M using the slides.

Name thesis:degree_name

Doctor of Philosophy - PhD

Level thesis:degree_level

doctoral

Discipline thesis:degree_discipline

Chemistry

Grantor dc:publisher

University of British Columbia

Year dc:date

1993

Author dc:creator

• Melzak, Kathryn

Statement dc:rights

• For non-commercial purposes only, such as research, private study and education. Additional conditions apply, see Terms of Use https://open.library.ubc.ca/terms_of_use.

Language dc:language

eng

Handle dc:identifier

http://hdl.handle.net/2429/1853

OAI identifier oai:identifier

oai:circle.library.ubc.ca:2429/1853