{"id":{"repo_id":"ubc","oai_identifier":"oai:circle.library.ubc.ca:2429/1361"},"canonical_url":"https://search.dev.ndltd.org/etd/ubc/oai:circle.library.ubc.ca:2429/1361","repository":{"repo_id":"ubc","name":"University of British Columbia","base_url":"http://circle.library.ubc.ca/oai/request"},"display":{"title":"Electrical stimulation of reward sites in the ventral tegmental area of the rat increases dopamine transmission in the nucleus accumbens as measured by in vivo microdialysis","abstract":"In vivo microdialysis with HPLC-ED was used to measure dopamine (DA),3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) in the nucleus accumbens of the rat, prior to, during, and after 15-min periods of electrical brain-stimulation at sites in the ventral tegmental area (VTA) that supported intracranial self-stimulation (ICSS). In the first experiment, both ICSS and yoked-stimulation of the VTA evoked significant increases in extracellular concentrations of DA, its metabolites, and 5-HIAA.Comparable results from ICSS and yoked groups were interpreted as evidence that the rewarding properties of VTA stimulation were a causal factor in the elevated DA transmission in the nucleus accumbens, rather than intense operant behavior. Further evidence for this hypothesis came from a second set of data in which changes in extracellular DA levels during the measurement of rate/intensity functions for ICSS were positively correlated. 5-HIAA concentrations also increased during ICSS but these changes were not correlated with either ICSS rate or current intensity, suggesting that changes in serotonin metabolism were unlikely to subserve brain-stimulation reward in the VTA. These results provide further evidence that stimulated mesolimbic DA release is a sufficient condition for reward.","abstract_html":"In vivo microdialysis with HPLC-ED was used to measure dopamine (DA),3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) in the nucleus accumbens of the rat, prior to, during, and after 15-min periods of electrical brain-stimulation at sites in the ventral tegmental area (VTA) that supported intracranial self-stimulation (ICSS). In the first experiment, both ICSS and yoked-stimulation of the VTA evoked significant increases in extracellular concentrations of DA, its metabolites, and 5-HIAA.Comparable results from ICSS and yoked groups were interpreted as evidence that the rewarding properties of VTA stimulation were a causal factor in the elevated DA transmission in the nucleus accumbens, rather than intense operant behavior. Further evidence for this hypothesis came from a second set of data in which changes in extracellular DA levels during the measurement of rate/intensity functions for ICSS were positively correlated. 5-HIAA concentrations also increased during ICSS but these changes were not correlated with either ICSS rate or current intensity, suggesting that changes in serotonin metabolism were unlikely to subserve brain-stimulation reward in the VTA. These results provide further evidence that stimulated mesolimbic DA release is a sufficient condition for reward.","abstract_has_math":false,"creators":["Fiorino, Dennis Frank"],"institution":"University of British Columbia","degree_name":"Master of Science - MSc","degree_level":"master's","degree_discipline":"Neuroscience","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1993,"date_issued":"1993","date_published":"1993","updated_at":"2026-07-24T05:07:17Z","subjects":[],"languages":["eng"],"rights":["For non-commercial purposes only, such as research, private study and education. 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In the first experiment, both ICSS and yoked-stimulation of the VTA evoked significant increases in extracellular concentrations of DA, its metabolites, and 5-HIAA.Comparable results from ICSS and yoked groups were interpreted as evidence that the rewarding properties of VTA stimulation were a causal factor in the elevated DA transmission in the nucleus accumbens, rather than intense operant behavior. Further evidence for this hypothesis came from a second set of data in which changes in extracellular DA levels during the measurement of rate/intensity functions for ICSS were positively correlated. 5-HIAA concentrations also increased during ICSS but these changes were not correlated with either ICSS rate or current intensity, suggesting that changes in serotonin metabolism were unlikely to subserve brain-stimulation reward in the VTA. These results provide further evidence that stimulated mesolimbic DA release is a sufficient condition for reward."]},{"key":"dc:format","label":"Dc Format","values":["2796917","application/pdf"]},{"key":"dc:title","label":"Title","values":["Electrical stimulation of reward sites in the ventral tegmental area of the rat increases dopamine transmission in the nucleus accumbens as measured by in vivo microdialysis"]}]}],"canonical_facts":{"dc:creator":["Fiorino, Dennis Frank"],"dc:date":["1993"],"dc:description":["In vivo microdialysis with HPLC-ED was used to measure dopamine (DA),3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) in the nucleus accumbens of the rat, prior to, during, and after 15-min periods of electrical brain-stimulation at sites in the ventral tegmental area (VTA) that supported intracranial self-stimulation (ICSS). In the first experiment, both ICSS and yoked-stimulation of the VTA evoked significant increases in extracellular concentrations of DA, its metabolites, and 5-HIAA.Comparable results from ICSS and yoked groups were interpreted as evidence that the rewarding properties of VTA stimulation were a causal factor in the elevated DA transmission in the nucleus accumbens, rather than intense operant behavior. Further evidence for this hypothesis came from a second set of data in which changes in extracellular DA levels during the measurement of rate/intensity functions for ICSS were positively correlated. 5-HIAA concentrations also increased during ICSS but these changes were not correlated with either ICSS rate or current intensity, suggesting that changes in serotonin metabolism were unlikely to subserve brain-stimulation reward in the VTA. 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