Observations of the growth of two strains of Trichomonas augusta from amphibians
This study was begun with three purposes in mind. One was to develop a better medium for the cultivation of flagellate protozoans of the genus Trichomonas by varying the viscosity and nutrient content of the constituents. The second was to determine, by rates of division, the effects of various antibiotics upon axenic cultures, and the third, was to compare division rates in various media which did not contain antibiotics. Since the few axenic cultures which were obtained lasted a maximum of four hours, the emphasis of the problem was shifted to the behavior of Trichomonas augusta growing in association with bacteria and molds. Under these conditions, attention was focused upon the relationship of bacteria and molds to protozoan population peaks, time of decline, adaptation times, and longevity of cultures.
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