Assay development for use in drug discovery against Bovine Trichomoniasis

<p>Bovine trichomoniasis is a venereal disease that affects cattle. The causative agent of this disease is <em>Tritrichomonas foetus</em>, a flagellated protozoan. There is no current FDA approved treatment for this disease. The purpose of this study was to develop new compound screening assays that will make the discovery of new compounds faster and more accurate. The CellTiter-Glo Luminescent Cell Viability Assay, a high throughput screening (HTS) assay from Promega, was found to be as affective at measuring cytotoxicity as traditional assaying techniques. For the first time. preen florescent protein. a reporter gene used in cell viability assavs was successfully transformed into <em>T. foetus</em> for use in HTS systems. This study also identified new compounds that can potentially be used as new treatments for this disease.</p>

Name thesis:degree_name

Master of Science (M.S.)

Level thesis:degree_level

Thesis - Pacific Access Restricted

Discipline thesis:degree_discipline

Biological Sciences

Year dc:date.available

2007

Author dc:creator

• Schreiber, Kimberly C. M.

Contributors dc:contributor

• Kirkwood Land

• Trichomoniasis Treatment
• Drug development
• Drugs Design
• Life Sciences

Licence

• http://rightsstatements.org/vocab/InC/1.0/

Repository record dc:identifier

https://scholarlycommons.pacific.edu/uop_etds/650

OAI identifier oai:identifier

oai:scholarlycommons.pacific.edu:uop_etds-1649