University of the Pacific
Characterization of downstream target genes regulated by ABF-1 in different states of B cell development
Abstract
dc:description.abstract<p>The ABF-1 gene encodes for a protein that belongs to the basic helix-loop-helix family of transcription factors. ABF-1 mRNA molecules have been detected in the lymphoid tissues, which include the bone marrow, lymph nodes, and appendix, as well as transformed B cells lines infected with Epstein-Barr virus. This study investigates the role of ABF-1 in regulating downstream target genes in the human mature B cell line RAJI, as well as the plasma cell line, ARH-77. Quantitative real-time polymerase chain reaction and DNA microarray technology was used to investigate target genes that are subjected to transcriptional regulation by ABF-1. Using ABF-1 inducible cell lines or B cell lines that overexpress ABF -1 by transient transfection experiments, we discovered many cellular genes that change in their transcriptional profiles in response to ABF -1 expression. Based upon the analysis of genes being affected following ABF-1 induction, our results support the hypothesis that ABF-1 primarily functions as a transcriptional repressor in vivo. Many genes that regulate the cellular processes of apoptosis, as well as the cell cycle, were repressed following ABF-1 expression. Because EBV has been reported to control ABF-1 gene expression, the identification of downstream target genes regulated by ABF-1 may provide insight into the molecular events that follow after EBV infection.</p>
Degree
thesis:*- Name thesis:degree_name
- Master of Science (M.S.)
- Level thesis:degree_level
- Thesis - Pacific Access Restricted
- Discipline thesis:degree_discipline
- Biological Sciences
- Year dc:date.available
- 2005
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Eusebio, Anthony R.
- Contributors dc:contributor
-
- Craig A. Vierra
Subjects
dc:subject × 4Rights
dc:rightsIdentifiers
dc:identifier.*- Repository record dc:identifier
- https://scholarlycommons.pacific.edu/uop_etds/618
- OAI identifier oai:identifier
- oai:scholarlycommons.pacific.edu:uop_etds-1617