University of the Pacific
Toxicology and pharmacology of N¹-acetylspermidine and N⁸-acetylspermidine
Abstract
dc:description.abstract<p>The polyamines are a group of natural compounds which have been found in almost all living tissues. N<sup>1</sup>- and N<sup>8</sup>- acetylspermidine have been known for a considerable time as normal constituents in human urine, but their physiological role is unknown. Recent studies have indicated the presence of enzymes in the tissues capable of converting spermidine into N<sup>1</sup>-acetylspermidine and N<sup>8</sup>-acetylspermidine and other enzyme activities which catalyze deacetylation and interconversion reactions.</p><p>One approach for determining physiologic activity of an endogenous compound is to observe their pharmacologic and toxicologic effects. In the present study, the LD<sub>50</sub> for N<sup>1</sup>-acetylspermidine, N<sup>8</sup>-acetylspermidine, spermidine, spermine and putrescine, were determined by intraperitoneal injection in mice. The LD<sub>50</sub> for N<sup>1</sup>-acetylspermidine •2HCl and N<sup>8</sup>-acetylspermidine •2HCl were 1150 and 820 mg/kg, respectively, and the respective LD<sub>50</sub> for spermidine •3HCl, spermine n#8226;4HCl and putrescine n#8226;2HCl were 870, 370, and 1400 mg/kg.</p><p>The major difference between the toxicity of N<sup>1</sup>-acetylspermidine and N<sup>8</sup>acetylspermidine and that of spermidine, spermine and putrescine appeared to be the latency period for the time of death. While N<sup>1</sup>- and N<sup>8</sup>-acetylspermidine have very rapid effects in which animals died in the first ten minutes after the injection with very few delayed deaths, the effects of spermidine, spermine and putrescine occurred over a wider range of time intervals with some deaths occurring as late as ten days after treatment</p><p>The other signs of toxicity with N<sup>1</sup>acetylspermidine, N<sup>8</sup>-acetylspermidine, spermidine, spermine, and putrescine were qualitatively quite similar as each compound produced hypothermia, sedation, muscle incoordination, decreased motor activity, decreased respiration, and clonic convulsions.</p>
Degree
thesis:*- Name thesis:degree_name
- Master of Science (M.S.)
- Level thesis:degree_level
- Thesis
- Discipline thesis:degree_discipline
- Graduate School
- Year dc:date.available
- 1981
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Alshabanah, Othman A.
- Contributors dc:contributor
-
- James Blankenship
Subjects
dc:subject × 4Rights
dc:rightsIdentifiers
dc:identifier.*- Repository record dc:identifier
- https://scholarlycommons.pacific.edu/uop_etds/456
- OAI identifier oai:identifier
- oai:scholarlycommons.pacific.edu:uop_etds-1455