{"id":{"repo_id":"twu","oai_identifier":"oai:twu-ir.tdl.org:11274/13795"},"canonical_url":"https://search.dev.ndltd.org/etd/twu/oai:twu-ir.tdl.org:11274/13795","repository":{"repo_id":"twu","name":"Texas Woman's University","base_url":"https://twu-ir.tdl.org/server/oai/request"},"display":{"title":"Synthesis of doxorubicin-albumin conjugates via cobalt coordination chemistry: The effect of reaction conditions on overall protein stability","abstract":"The applications of nanotechnology in designing better treatments shows great promise in lessening the burden of chemotherapy while increasing therapeutic effect. Protein-drug conjugates are a rapidly expanding family of therapeutics that hold potential for ameliorating off-target toxic effects observed in chemotherapy. In this context, human serum albumin could act as a nanoscale delivery vector to alter the biodistribution of attached therapeutics. This research explored the use of cobalt coordination chemistry in the synthesis of Doxalbumin conjugates. Doxorubicin was chosen because it contains a primary amine that could be crosslinked to protein using cobalt coordination chemistry. The effect of pH and reaction time on the synthesis of conjugates was investigated. Samples were characterized by High Performance Liquid Chromatography, Dynamic Light Scattering, and Differential Scanning Calorimetry. Optimized reaction conditions for synthesizing conjugates with varying numbers of dox molecules per protein was a major focus of the research.","abstract_html":"The applications of nanotechnology in designing better treatments shows great promise in lessening the burden of chemotherapy while increasing therapeutic effect. Protein-drug conjugates are a rapidly expanding family of therapeutics that hold potential for ameliorating off-target toxic effects observed in chemotherapy. In this context, human serum albumin could act as a nanoscale delivery vector to alter the biodistribution of attached therapeutics. This research explored the use of cobalt coordination chemistry in the synthesis of Doxalbumin conjugates. Doxorubicin was chosen because it contains a primary amine that could be crosslinked to protein using cobalt coordination chemistry. The effect of pH and reaction time on the synthesis of conjugates was investigated. Samples were characterized by High Performance Liquid Chromatography, Dynamic Light Scattering, and Differential Scanning Calorimetry. Optimized reaction conditions for synthesizing conjugates with varying numbers of dox molecules per protein was a major focus of the research.","abstract_has_math":false,"creators":["Patel, Ashik"],"institution":"Texas Woman's University","degree_name":"Master of Science","degree_level":"Masters","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":[],"advisors":["Petros, Robby"],"committee_chairs":[],"committee_members":["Salazar, Gustavo","Sheardy, Richard"],"year":2022,"date_issued":"3/30/2022","date_published":"3/30/2022","updated_at":"2026-07-24T05:05:09Z","subjects":["Cancer research","Chemotherapeutic drugs","Nanomaterials"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/11274/13795","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Petros, Robby"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Salazar, Gustavo","Sheardy, Richard"]},{"key":"dc:creator","label":"Author","values":["Patel, Ashik"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2022-06-28T21:08:14Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2022-06-28T21:08:14Z"]},{"key":"dc:date.issued","label":"Date","values":["3/30/2022"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Texas Woman's University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Cancer research","Chemotherapeutic drugs","Nanomaterials"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/11274/13795"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The applications of nanotechnology in designing better treatments shows great promise in lessening the burden of chemotherapy while increasing therapeutic effect. Protein-drug conjugates are a rapidly expanding family of therapeutics that hold potential for ameliorating off-target toxic effects observed in chemotherapy. In this context, human serum albumin could act as a nanoscale delivery vector to alter the biodistribution of attached therapeutics. This research explored the use of cobalt coordination chemistry in the synthesis of Doxalbumin conjugates. Doxorubicin was chosen because it contains a primary amine that could be crosslinked to protein using cobalt coordination chemistry. The effect of pH and reaction time on the synthesis of conjugates was investigated. Samples were characterized by High Performance Liquid Chromatography, Dynamic Light Scattering, and Differential Scanning Calorimetry. Optimized reaction conditions for synthesizing conjugates with varying numbers of dox molecules per protein was a major focus of the research."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Synthesis of doxorubicin-albumin conjugates via cobalt coordination chemistry: The effect of reaction conditions on overall protein stability"]}]}],"canonical_facts":{"dc:contributor.advisor":["Petros, Robby"],"dc:contributor.committeemember":["Salazar, Gustavo","Sheardy, Richard"],"dc:creator":["Patel, Ashik"],"dc:date.accessioned":["2022-06-28T21:08:14Z"],"dc:date.available":["2022-06-28T21:08:14Z"],"dc:date.issued":["3/30/2022"],"dc:description.abstract":["The applications of nanotechnology in designing better treatments shows great promise in lessening the burden of chemotherapy while increasing therapeutic effect. Protein-drug conjugates are a rapidly expanding family of therapeutics that hold potential for ameliorating off-target toxic effects observed in chemotherapy. In this context, human serum albumin could act as a nanoscale delivery vector to alter the biodistribution of attached therapeutics. This research explored the use of cobalt coordination chemistry in the synthesis of Doxalbumin conjugates. Doxorubicin was chosen because it contains a primary amine that could be crosslinked to protein using cobalt coordination chemistry. The effect of pH and reaction time on the synthesis of conjugates was investigated. Samples were characterized by High Performance Liquid Chromatography, Dynamic Light Scattering, and Differential Scanning Calorimetry. Optimized reaction conditions for synthesizing conjugates with varying numbers of dox molecules per protein was a major focus of the research."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["https://hdl.handle.net/11274/13795"],"dc:subject":["Cancer research","Chemotherapeutic drugs","Nanomaterials"],"dc:title":["Synthesis of doxorubicin-albumin conjugates via cobalt coordination chemistry: The effect of reaction conditions on overall protein stability"],"dc:type":["Thesis"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Texas Woman's University"]},"updated_at":"2026-07-24T05:05:09Z"}