Endophytes as source of polypeptide and polyketide antibiotics fengycin and bacillaene
type: CreativeWork
description: Endophytic bacteria, which populate plant tissues, can influence physiological processes in plants. They also have antagonistic effects against many phytopathogens. Utilization of these bacteria as a biological agent for protection of plants is an environmentally friendly alternative to chemical methods. Moreover, since plants belong to the eukaryotic organisms, antibiotics produced by endophytes may have reduced cell toxicity. Therefore, the antimicrobial compounds isolated from endophytes have a big potential for the pharmaceutical industry and agriculture. Potato plants were chosen as source of endophytes due to their ability to protect themselves from a broad spectrum of phytopathogens. Several bacterial strains having antifungal activities were isolated from potato tubers. One strain, which exhibited the strongest fungicide potential, was characterized and identified as Bacillus subtilis subsp. subtilis. It was deposited under the number DSM 21393 at the public collection of the DSMZ GmbH (Germany). The substances exhibiting antifungal activities were purified and after structure elucidation identified as the lipopeptide antibiotic fengycin. Due to its very strong fungicide as well as bactericide and anti-cancer properties this antibiotic has a good potential for applications in the pharmaceutical industry and agriculture. Furthermore, it exhibits strong surface active properties, which makes it of great interest as biosurfactant. Despite the great potential of lipopeptides, the industrial use of these antibiotics is still limited due to their high production costs, which result primarily from low strain productivity and difficulties in bioprocess design as well as downstream processing. Therefore, a further aim of this project was the development of a cost-effective production process for fengycin. Different complex substrates such as malt-, potato extracts and molasses as well as their mixtures were used as culture media. The maximum fengycin yield of 325mg/L…
abstract: Endophytic bacteria, which populate plant tissues, can influence physiological processes in plants. They also have antagonistic effects against many phytopathogens. Utilization of these bacteria as a biological agent for protection of plants is an environmentally friendly alternative to chemical methods. Moreover, since plants belong to the eukaryotic organisms, antibiotics produced by endophytes may have reduced cell toxicity. Therefore, the antimicrobial compounds isolated from endophytes have a big potential for the pharmaceutical industry and agriculture. Potato plants were chosen as source of endophytes due to their ability to protect themselves from a broad spectrum of phytopathogens. Several bacterial strains having antifungal activities were isolated from potato tubers. One strain, which exhibited the strongest fungicide potential, was characterized and identified as Bacillus subtilis subsp. subtilis. It was deposited under the number DSM 21393 at the public collection of the DSMZ GmbH (Germany). The substances exhibiting antifungal activities were purified and after structure elucidation identified as the lipopeptide antibiotic fengycin. Due to its very strong fungicide as well as bactericide and anti-cancer properties this antibiotic has a good potential for applications in the pharmaceutical industry and agriculture. Furthermore, it exhibits strong surface active properties, which makes it of great interest as biosurfactant. Despite the great potential of lipopeptides, the industrial use of these antibiotics is still limited due to their high production costs, which result primarily from low strain productivity and difficulties in bioprocess design as well as downstream processing. Therefore, a further aim of this project was the development of a cost-effective production process for fengycin. Different complex substrates such as malt-, potato extracts and molasses as well as their mixtures were used as culture media. The maximum fengycin yield of 325mg/L…
author: Glazyrina, Julia
language: en
license: https://creativecommons.org/licenses/by/4.0/
date published: 2018
date modified: 2026-07-27T21:28:40Z
provider: Technische Universität Berlin
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Technische Universität Berlin

PropertyValue
property ID: DOI
value: 10.14279/depositonce-6714