Advanced Raman spectroscopy for bioprocess monitoring
type: CreativeWork
description: The Raman -effect was discovered almost 90 years ago. It took a long time until the importance of Raman spectroscopy was fully understood and accepted for process industrial applications. Still today the usage is limited to a small application area. During the last decades advances in the production of spectroscopic components have reduced the complexity of the instrumentation and further mediated a persistent decline in costs, thus making this technology available for a broader audience. The basic components of a Raman spectrometer are a monochromatic light source, typically laser, a Raman probe with optical fibres, a spectrograph and a detector which is connected to a measurement- and control PC. Raman spectroscopy is increasingly becoming a choice as analytical tool in bioanalytics. Most of biological samples are handled in aqueous form which challenges many other analytical techniques (e.g., infrared spectroscopy). Raman and its enhancement techniques are able to measure quality and quantity of compounds in liquid phase with no or very little interference of water. The quantity of a compound can be determined by the peak-intensity and the quality by the position in the measured spectrum. Another advantage is that Raman spectroscopy does not rely on extensive sample preparation and measurements can be carried out non-invasively by placing an immersion probe with fibre optics directly in the liquid media. A Raman measurement is conducted fast, within milliseconds, and multiple relevant process parameters from the same sample can be measured at the same time. The measurements can be performed in continuous mode, i.e. one after the other or with a delay in-between. The operator can determine the measurement interval and in this way the development of a process can be observed online and in real-time. Further important advantages of spectroscopic methods over many other biochemical and physical measurement tools are the robustness as they do not require assays and th…
abstract: The Raman -effect was discovered almost 90 years ago. It took a long time until the importance of Raman spectroscopy was fully understood and accepted for process industrial applications. Still today the usage is limited to a small application area. During the last decades advances in the production of spectroscopic components have reduced the complexity of the instrumentation and further mediated a persistent decline in costs, thus making this technology available for a broader audience. The basic components of a Raman spectrometer are a monochromatic light source, typically laser, a Raman probe with optical fibres, a spectrograph and a detector which is connected to a measurement- and control PC. Raman spectroscopy is increasingly becoming a choice as analytical tool in bioanalytics. Most of biological samples are handled in aqueous form which challenges many other analytical techniques (e.g., infrared spectroscopy). Raman and its enhancement techniques are able to measure quality and quantity of compounds in liquid phase with no or very little interference of water. The quantity of a compound can be determined by the peak-intensity and the quality by the position in the measured spectrum. Another advantage is that Raman spectroscopy does not rely on extensive sample preparation and measurements can be carried out non-invasively by placing an immersion probe with fibre optics directly in the liquid media. A Raman measurement is conducted fast, within milliseconds, and multiple relevant process parameters from the same sample can be measured at the same time. The measurements can be performed in continuous mode, i.e. one after the other or with a delay in-between. The operator can determine the measurement interval and in this way the development of a process can be observed online and in real-time. Further important advantages of spectroscopic methods over many other biochemical and physical measurement tools are the robustness as they do not require assays and th…
author: Kögler, Martin
language: en
license: http://rightsstatements.org/vocab/InC/1.0/
date published: 2018
date modified: 2026-07-27T21:28:57Z
provider: Technische Universität Berlin
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Technische Universität Berlin

PropertyValue
property ID: DOI
value: 10.14279/depositonce-6684