{"id":{"repo_id":"toronto-retro","oai_identifier":"oai:utoronto.scholaris.ca:1807/69414"},"canonical_url":"https://search.dev.ndltd.org/etd/toronto-retro/oai:utoronto.scholaris.ca:1807/69414","repository":{"repo_id":"toronto-retro","name":"University of Toronto","base_url":"https://utoronto.scholaris.ca/server/oai/request"},"display":{"title":"Primitive Neural Stem Cells in the Mouse Brain","abstract":"Neural stem cells (NSCs) reside in the tissue lining the lateral ventricles of the adult mouse brain. At the top of the hierarchy are primitive (p)NSCs that arise in advance of definitive (d)NSCs embryonically. After the discovery that pNSCs persist in the adult mouse brain, I sought out to characterize pNSCs and determine whether they express the pluripotency gene Oct4 in the adult brain as the do embryonically. Next, I addressed the cell cycle time of pNSCs and whether they are activated to proliferate to repopulate dNSCs after dNSC and downstream progenitor ablation. Finally, I identified cell type specific markers of pNSCs and pharmacological methods to selectively target and activate endogenous pNSCs. These selective markers can be used for future studies to enrich for pNSCs and to develop future therapies to target pNSCs endogenously. Together, this thesis presents evidence that pNSCs are an Oct4-expressing, reserve population at the top of the NSC hierarchy capable of repopulating dNSCs.","abstract_html":"Neural stem cells (NSCs) reside in the tissue lining the lateral ventricles of the adult mouse brain. At the top of the hierarchy are primitive (p)NSCs that arise in advance of definitive (d)NSCs embryonically. After the discovery that pNSCs persist in the adult mouse brain, I sought out to characterize pNSCs and determine whether they express the pluripotency gene Oct4 in the adult brain as the do embryonically. Next, I addressed the cell cycle time of pNSCs and whether they are activated to proliferate to repopulate dNSCs after dNSC and downstream progenitor ablation. Finally, I identified cell type specific markers of pNSCs and pharmacological methods to selectively target and activate endogenous pNSCs. These selective markers can be used for future studies to enrich for pNSCs and to develop future therapies to target pNSCs endogenously. Together, this thesis presents evidence that pNSCs are an Oct4-expressing, reserve population at the top of the NSC hierarchy capable of repopulating dNSCs.","abstract_has_math":false,"creators":["Reeve, Rachel Leeder"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":"Medical Science","school":null,"contributors":[],"advisors":["van der Kooy, Derek"],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-06","date_published":"2015-06","updated_at":"2026-07-27T21:27:54Z","subjects":["neural stem cells"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/1807/69414","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["van der Kooy, Derek"]},{"key":"dc:contributor.department","label":"Department","values":["Medical Science"]},{"key":"dc:creator","label":"Author","values":["Reeve, Rachel Leeder"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-06"]},{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2015-08-12T04:00:33Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2015-08-12T04:00:33Z"]},{"key":"dc:date.issued","label":"Date","values":["2015-06"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["neural stem cells"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/1807/69414"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Neural stem cells (NSCs) reside in the tissue lining the lateral ventricles of the adult mouse brain. At the top of the hierarchy are primitive (p)NSCs that arise in advance of definitive (d)NSCs embryonically. After the discovery that pNSCs persist in the adult mouse brain, I sought out to characterize pNSCs and determine whether they express the pluripotency gene Oct4 in the adult brain as the do embryonically. Next, I addressed the cell cycle time of pNSCs and whether they are activated to proliferate to repopulate dNSCs after dNSC and downstream progenitor ablation. Finally, I identified cell type specific markers of pNSCs and pharmacological methods to selectively target and activate endogenous pNSCs. These selective markers can be used for future studies to enrich for pNSCs and to develop future therapies to target pNSCs endogenously. Together, this thesis presents evidence that pNSCs are an Oct4-expressing, reserve population at the top of the NSC hierarchy capable of repopulating dNSCs."]},{"key":"dc:description.degree","label":"Dc Description Degree","values":["Ph.D."]},{"key":"dc:title","label":"Title","values":["Primitive Neural Stem Cells in the Mouse Brain"]}]}],"canonical_facts":{"dc:contributor.advisor":["van der Kooy, Derek"],"dc:contributor.department":["Medical Science"],"dc:creator":["Reeve, Rachel Leeder"],"dc:date":["2015-06"],"dc:date.accessioned":["2015-08-12T04:00:33Z"],"dc:date.available":["2015-08-12T04:00:33Z"],"dc:date.issued":["2015-06"],"dc:description.abstract":["Neural stem cells (NSCs) reside in the tissue lining the lateral ventricles of the adult mouse brain. At the top of the hierarchy are primitive (p)NSCs that arise in advance of definitive (d)NSCs embryonically. After the discovery that pNSCs persist in the adult mouse brain, I sought out to characterize pNSCs and determine whether they express the pluripotency gene Oct4 in the adult brain as the do embryonically. Next, I addressed the cell cycle time of pNSCs and whether they are activated to proliferate to repopulate dNSCs after dNSC and downstream progenitor ablation. Finally, I identified cell type specific markers of pNSCs and pharmacological methods to selectively target and activate endogenous pNSCs. These selective markers can be used for future studies to enrich for pNSCs and to develop future therapies to target pNSCs endogenously. Together, this thesis presents evidence that pNSCs are an Oct4-expressing, reserve population at the top of the NSC hierarchy capable of repopulating dNSCs."],"dc:description.degree":["Ph.D."],"dc:identifier.uri":["http://hdl.handle.net/1807/69414"],"dc:subject":["neural stem cells"],"dc:title":["Primitive Neural Stem Cells in the Mouse Brain"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T21:27:54Z"}