{"id":{"repo_id":"the-open-u","oai_identifier":"oai:oro.open.ac.uk:54861"},"canonical_url":"https://search.dev.ndltd.org/etd/the-open-u/oai:oro.open.ac.uk:54861","repository":{"repo_id":"the-open-u","name":"The Open University","base_url":"https://oro.open.ac.uk/cgi/oai2"},"display":{"title":"Investigation of dendritic cells as a vector for vaccine delivery","abstract":"Dendritic cells (DC) are an attractive target for both vaccine targeting and use as a delivery vehicle due to their ability to direct innate and adaptive immune responses. In this study a procedure was developed where DC were employed as a vehicle to induce cell-mediated immune responses to the Gram negative bacillus <i>Burkholderia pseudomallei</i>. This bacterium is the causative agent of melioidosis, a disease endemicto areas of south-east Asia and northern Australia, to which presently no licensed vaccine exists.<br></br><br></br>Purified DC were pulsed with heat-killed (HK) whole cell <i>B. pseudomallei</i> and used to immunise syngeneic mice. The use of this immunisation method elicited strong cellular immune responses against <i>B. pseudomallei</i>, which have historically been difficult to generate. Antibody responses following immunisation with DC were low. Booster immunisations of either a second dose of HK <i>B. pseudomallei</i> pulsed DC or HK <i>B.pseudomallei</i> in MPL + TDM adjuvant were administered to increase the immuneresponse. Immunised animals were challenged with fully virulent <i>B. pseudomallei</i> and protection was demonstrated in animals immunised with a combination of HK <i>B. pseudomallei</i> pulsed DC and HK <i>B. pseudomallei</i> delivered in MPL + TDM adjuvant. The protective immune response demonstrated was then further elucidated to reveal arole for both cell-mediated and humoral immunity in protection against <i>B. pseudomallei</i> infection. The data also demonstrate the potential for DC as vaccine delivery vectors and as a screening mechanism for the evaluation of potential vaccine candidates.","abstract_html":"Dendritic cells (DC) are an attractive target for both vaccine targeting and use as a delivery vehicle due to their ability to direct innate and adaptive immune responses. In this study a procedure was developed where DC were employed as a vehicle to induce cell-mediated immune responses to the Gram negative bacillus &lt;i&gt;Burkholderia pseudomallei&lt;/i&gt;. This bacterium is the causative agent of melioidosis, a disease endemicto areas of south-east Asia and northern Australia, to which presently no licensed vaccine exists.&lt;br&gt;&lt;/br&gt;&lt;br&gt;&lt;/br&gt;Purified DC were pulsed with heat-killed (HK) whole cell &lt;i&gt;B. pseudomallei&lt;/i&gt; and used to immunise syngeneic mice. The use of this immunisation method elicited strong cellular immune responses against &lt;i&gt;B. pseudomallei&lt;/i&gt;, which have historically been difficult to generate. Antibody responses following immunisation with DC were low. Booster immunisations of either a second dose of HK &lt;i&gt;B. pseudomallei&lt;/i&gt; pulsed DC or HK &lt;i&gt;B.pseudomallei&lt;/i&gt; in MPL + TDM adjuvant were administered to increase the immuneresponse. Immunised animals were challenged with fully virulent &lt;i&gt;B. pseudomallei&lt;/i&gt; and protection was demonstrated in animals immunised with a combination of HK &lt;i&gt;B. pseudomallei&lt;/i&gt; pulsed DC and HK &lt;i&gt;B. pseudomallei&lt;/i&gt; delivered in MPL + TDM adjuvant. The protective immune response demonstrated was then further elucidated to reveal arole for both cell-mediated and humoral immunity in protection against &lt;i&gt;B. pseudomallei&lt;/i&gt; infection. The data also demonstrate the potential for DC as vaccine delivery vectors and as a screening mechanism for the evaluation of potential vaccine candidates.","abstract_has_math":false,"creators":["Healey, Gareth D."],"institution":"The Open University","degree_name":"phd","degree_level":"doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2005,"date_issued":"2005","date_published":"2005","updated_at":"2026-07-24T05:02:49Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Healey, Gareth D."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2005"]},{"key":"dc:date.issued","label":"Date","values":["2005"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["The Open University"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://oro.open.ac.uk/54861/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["phd"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://oro.open.ac.uk/54861/1/418466.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Dendritic cells (DC) are an attractive target for both vaccine targeting and use as a delivery vehicle due to their ability to direct innate and adaptive immune responses. In this study a procedure was developed where DC were employed as a vehicle to induce cell-mediated immune responses to the Gram negative bacillus <i>Burkholderia pseudomallei</i>. This bacterium is the causative agent of melioidosis, a disease endemicto areas of south-east Asia and northern Australia, to which presently no licensed vaccine exists.<br></br><br></br>Purified DC were pulsed with heat-killed (HK) whole cell <i>B. pseudomallei</i> and used to immunise syngeneic mice. The use of this immunisation method elicited strong cellular immune responses against <i>B. pseudomallei</i>, which have historically been difficult to generate. Antibody responses following immunisation with DC were low. Booster immunisations of either a second dose of HK <i>B. pseudomallei</i> pulsed DC or HK <i>B.pseudomallei</i> in MPL + TDM adjuvant were administered to increase the immuneresponse. Immunised animals were challenged with fully virulent <i>B. pseudomallei</i> and protection was demonstrated in animals immunised with a combination of HK <i>B. pseudomallei</i> pulsed DC and HK <i>B. pseudomallei</i> delivered in MPL + TDM adjuvant. The protective immune response demonstrated was then further elucidated to reveal arole for both cell-mediated and humoral immunity in protection against <i>B. pseudomallei</i> infection. The data also demonstrate the potential for DC as vaccine delivery vectors and as a screening mechanism for the evaluation of potential vaccine candidates."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Investigation of dendritic cells as a vector for vaccine delivery"]}]}],"canonical_facts":{"dc:creator":["Healey, Gareth D."],"dc:date":["2005"],"dc:date.issued":["2005"],"dc:description.abstract":["Dendritic cells (DC) are an attractive target for both vaccine targeting and use as a delivery vehicle due to their ability to direct innate and adaptive immune responses. In this study a procedure was developed where DC were employed as a vehicle to induce cell-mediated immune responses to the Gram negative bacillus <i>Burkholderia pseudomallei</i>. This bacterium is the causative agent of melioidosis, a disease endemicto areas of south-east Asia and northern Australia, to which presently no licensed vaccine exists.<br></br><br></br>Purified DC were pulsed with heat-killed (HK) whole cell <i>B. pseudomallei</i> and used to immunise syngeneic mice. The use of this immunisation method elicited strong cellular immune responses against <i>B. pseudomallei</i>, which have historically been difficult to generate. Antibody responses following immunisation with DC were low. Booster immunisations of either a second dose of HK <i>B. pseudomallei</i> pulsed DC or HK <i>B.pseudomallei</i> in MPL + TDM adjuvant were administered to increase the immuneresponse. Immunised animals were challenged with fully virulent <i>B. pseudomallei</i> and protection was demonstrated in animals immunised with a combination of HK <i>B. pseudomallei</i> pulsed DC and HK <i>B. pseudomallei</i> delivered in MPL + TDM adjuvant. The protective immune response demonstrated was then further elucidated to reveal arole for both cell-mediated and humoral immunity in protection against <i>B. pseudomallei</i> infection. The data also demonstrate the potential for DC as vaccine delivery vectors and as a screening mechanism for the evaluation of potential vaccine candidates."],"dc:format":["application/pdf"],"dc:identifier.uri":["https://oro.open.ac.uk/54861/1/418466.pdf"],"dc:language":["en"],"dc:publisher.institution":["The Open University"],"dc:relation.isreferencedby":["https://oro.open.ac.uk/54861/"],"dc:title":["Investigation of dendritic cells as a vector for vaccine delivery"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["doctoral"],"dc:type.qualificationname":["phd"]},"updated_at":"2026-07-24T05:02:49Z"}