{"id":{"repo_id":"the-open-u","oai_identifier":"oai:oro.open.ac.uk:53696"},"canonical_url":"https://search.dev.ndltd.org/etd/the-open-u/oai:oro.open.ac.uk:53696","repository":{"repo_id":"the-open-u","name":"The Open University","base_url":"https://oro.open.ac.uk/cgi/oai2"},"display":{"title":"Regulation Of Transcription By RNA Polymerase II In S. pombe","abstract":"An understanding of the mechanisms underlying the various stages of transcription is crucial to find solutions to the problems caused by mis-expression of genes that may give rise to a host of human diseases. My thesis research focusses on an analysis of the RNA Polymerase II elongation factor Ell1/Eaf1 in S. pombe. Eleven-nineteen lysine-rich in leukemia (ELL) is encoded by a gene involved in translocations with MLL in leukemia and forms a tight complex with ELL-associated factors (EAF). ELL/EAF is an RNA polymerase II elongation factor that in metazoa can assemble into a larger assembly that also includes P-TEFb and other proteins encoded by genes involved in MLL translocations. This larger assembly, sometimes called SEC, binds to a specific \"docking site\" in the metazoan Mediator complex. Distantly related ELL- and EAF-like genes were identified in S. pombe that encode Ell1/Eaf1 and can stimulate Pol II elongation in vitro. My thesis addresses two distinct projects, with overlapping motivations:
First, to see whether S. pombe might provide a good model for functional studies of the Mediator and ELL/EAF interaction, I carried out a thorough proteomic analysis of S. pombe Mediator and defined several new subunits. My results were recently published as part of a collaborative structural analysis of S. pombe Mediator.
Second, I used a combination of biochemical, genetic, and genomic approaches to characterize Ell1/Eaf1 function in fission yeast. Using mass spectrometry, I identified an uncharacterized sequence orphan, SPAC6G9.15c, that associates with both Ell1 and Eaf1 to form a ternary complex that, based on ChIP-seq localizes at genes with high RNA Pol II occupancy. I also performed an SGA screen for genes that genetically interact with ell1, eaf1, and SPAC6G9.15c and identified a set of overlapping genes that interact with all three, as well as others that interact only with ell1.","abstract_html":"An understanding of the mechanisms underlying the various stages of transcription is crucial to find solutions to the problems caused by mis-expression of genes that may give rise to a host of human diseases. My thesis research focusses on an analysis of the RNA Polymerase II elongation factor Ell1/Eaf1 in <i>S. pombe</i>. Eleven-nineteen lysine-rich in leukemia (ELL) is encoded by a gene involved in translocations with MLL in leukemia and forms a tight complex with ELL-associated factors (EAF). ELL/EAF is an RNA polymerase II elongation factor that in metazoa can assemble into a larger assembly that also includes P-TEFb and other proteins encoded by genes involved in MLL translocations. This larger assembly, sometimes called SEC, binds to a specific "docking site" in the metazoan Mediator complex. Distantly related ELL- and EAF-like genes were identified in <i>S. pombe</i> that encode Ell1/Eaf1 and can stimulate Pol II elongation <i>in vitro</i>. My thesis addresses two distinct projects, with overlapping motivations: <br></br><br></br> First, to see whether <i>S. pombe</i> might provide a good model for functional studies of the Mediator and ELL/EAF interaction, I carried out a thorough proteomic analysis of <i>S. pombe</i> Mediator and defined several new subunits. My results were recently published as part of a collaborative structural analysis of <i>S. pombe</i> Mediator. <br></br><br></br> Second, I used a combination of biochemical, genetic, and genomic approaches to characterize Ell1/Eaf1 function in fission yeast. Using mass spectrometry, I identified an uncharacterized sequence orphan, <i>SPAC6G9.15c</i>, that associates with both Ell1 and Eaf1 to form a ternary complex that, based on ChIP-seq localizes at genes with high RNA Pol II occupancy. I also performed an SGA screen for genes that genetically interact with <i>ell1, eaf1</i>, and <i>SPAC6G9.15c</i> and identified a set of overlapping genes that interact with all three, as well as others that interact only with <i>ell1</i>.","abstract_has_math":false,"creators":["Gopalan, Sneha"],"institution":"The Open University","degree_name":"phd","degree_level":"doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-02","date_published":"2018-02","updated_at":"2026-07-24T05:02:39Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Gopalan, Sneha"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-02-27"]},{"key":"dc:date.issued","label":"Date","values":["2018-02"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["ARRAY(0x7ffb744aa7c8)"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["The Open University"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://oro.open.ac.uk/53696/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["phd"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://oro.open.ac.uk/53696/1/SNG_thesis-final-pages.pdf","https://oro.open.ac.uk/53696/7/GRADPROG%20memo-%20ARC.docx","https://oro.open.ac.uk/53696/8/Library%20deposition%20memo%20%282%29.doc","https://oro.open.ac.uk/53696/9/thesis%20deposition%20form.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["An understanding of the mechanisms underlying the various stages of transcription is crucial to find solutions to the problems caused by mis-expression of genes that may give rise to a host of human diseases. My thesis research focusses on an analysis of the RNA Polymerase II elongation factor Ell1/Eaf1 in S. pombe. Eleven-nineteen lysine-rich in leukemia (ELL) is encoded by a gene involved in translocations with MLL in leukemia and forms a tight complex with ELL-associated factors (EAF). ELL/EAF is an RNA polymerase II elongation factor that in metazoa can assemble into a larger assembly that also includes P-TEFb and other proteins encoded by genes involved in MLL translocations. This larger assembly, sometimes called SEC, binds to a specific \"docking site\" in the metazoan Mediator complex. Distantly related ELL- and EAF-like genes were identified in S. pombe that encode Ell1/Eaf1 and can stimulate Pol II elongation in vitro. My thesis addresses two distinct projects, with overlapping motivations:
First, to see whether S. pombe might provide a good model for functional studies of the Mediator and ELL/EAF interaction, I carried out a thorough proteomic analysis of S. pombe Mediator and defined several new subunits. My results were recently published as part of a collaborative structural analysis of S. pombe Mediator.
Second, I used a combination of biochemical, genetic, and genomic approaches to characterize Ell1/Eaf1 function in fission yeast. Using mass spectrometry, I identified an uncharacterized sequence orphan, SPAC6G9.15c, that associates with both Ell1 and Eaf1 to form a ternary complex that, based on ChIP-seq localizes at genes with high RNA Pol II occupancy. I also performed an SGA screen for genes that genetically interact with ell1, eaf1, and SPAC6G9.15c and identified a set of overlapping genes that interact with all three, as well as others that interact only with ell1."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf","application/vnd.openxmlformats-officedocument.wordprocessingml.document","application/msword"]},{"key":"dc:title","label":"Title","values":["Regulation Of Transcription By RNA Polymerase II In S. pombe"]}]}],"canonical_facts":{"dc:creator":["Gopalan, Sneha"],"dc:date":["2018-02-27"],"dc:date.issued":["2018-02"],"dc:description.abstract":["An understanding of the mechanisms underlying the various stages of transcription is crucial to find solutions to the problems caused by mis-expression of genes that may give rise to a host of human diseases. My thesis research focusses on an analysis of the RNA Polymerase II elongation factor Ell1/Eaf1 in S. pombe. Eleven-nineteen lysine-rich in leukemia (ELL) is encoded by a gene involved in translocations with MLL in leukemia and forms a tight complex with ELL-associated factors (EAF). ELL/EAF is an RNA polymerase II elongation factor that in metazoa can assemble into a larger assembly that also includes P-TEFb and other proteins encoded by genes involved in MLL translocations. This larger assembly, sometimes called SEC, binds to a specific \"docking site\" in the metazoan Mediator complex. Distantly related ELL- and EAF-like genes were identified in S. pombe that encode Ell1/Eaf1 and can stimulate Pol II elongation in vitro. My thesis addresses two distinct projects, with overlapping motivations:
First, to see whether S. pombe might provide a good model for functional studies of the Mediator and ELL/EAF interaction, I carried out a thorough proteomic analysis of S. pombe Mediator and defined several new subunits. My results were recently published as part of a collaborative structural analysis of S. pombe Mediator.
Second, I used a combination of biochemical, genetic, and genomic approaches to characterize Ell1/Eaf1 function in fission yeast. Using mass spectrometry, I identified an uncharacterized sequence orphan, SPAC6G9.15c, that associates with both Ell1 and Eaf1 to form a ternary complex that, based on ChIP-seq localizes at genes with high RNA Pol II occupancy. I also performed an SGA screen for genes that genetically interact with ell1, eaf1, and SPAC6G9.15c and identified a set of overlapping genes that interact with all three, as well as others that interact only with ell1."],"dc:format":["application/pdf","application/vnd.openxmlformats-officedocument.wordprocessingml.document","application/msword"],"dc:identifier.uri":["https://oro.open.ac.uk/53696/1/SNG_thesis-final-pages.pdf","https://oro.open.ac.uk/53696/7/GRADPROG%20memo-%20ARC.docx","https://oro.open.ac.uk/53696/8/Library%20deposition%20memo%20%282%29.doc","https://oro.open.ac.uk/53696/9/thesis%20deposition%20form.pdf"],"dc:language":["en"],"dc:publisher.department":["ARRAY(0x7ffb744aa7c8)"],"dc:publisher.institution":["The Open University"],"dc:relation.isreferencedby":["https://oro.open.ac.uk/53696/"],"dc:title":["Regulation Of Transcription By RNA Polymerase II In S. pombe"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["doctoral"],"dc:type.qualificationname":["phd"]},"updated_at":"2026-07-24T05:02:39Z"}