{"id":{"repo_id":"the-open-u","oai_identifier":"oai:oro.open.ac.uk:52284"},"canonical_url":"https://search.dev.ndltd.org/etd/the-open-u/oai:oro.open.ac.uk:52284","repository":{"repo_id":"the-open-u","name":"The Open University","base_url":"https://oro.open.ac.uk/cgi/oai2"},"display":{"title":"A novel model system for the study of anti-tumour T-cell memory","abstract":"An adoptive immunotherapy (ADI) protocol was developed where the fate and requirements of longterm persisting memory T-cells can be monitored. Anti-tumour immune peritoneal exudate cells (iPEC) were produced by injecting a subtumorigenic dose of the highly metastatic, ß-gal<sup>+</sup> T-Iymphoma into the ear pinna of syngeneic DBN2 mice, followed by an intraperitoneal challenge with irradiation-inactivated tumour cells. 33.9 % of CD8<sup>+</sup> iPEC were shown to be recognising the immunodominant peptide of ß-gal (aa 876-884), and, consistently, the iPEC exerted specific lysis of ß-gal<sup>+</sup> cells. Upon adoptive transfer into sublethally irradiated, ESbL-Gal tumour-bearing, athymic Balb/c nu/nu mice, they conferred protective and long-lasting anti-tumour immunity. ADI-treated animals were able to reject subsequent high dose tumour challenges, and memory T-cells appeared to be only partially affected by y-irradiation. ß-gaI<sup>876-884</sup> peptide/MHC class I tetramer stainings identified the bone marrow as the major compartment for the long-term persistence of memory T-cells, as ß-gaI<sup>876-884</sup> specific T -cells occurred at elevated frequencies in this microenvironment as compared to the spleen and lymph nodes. In a \"parking experiment\", Agremoval led to a decrease of tetramer-binding cells below background levels. Tumour-reactive memory T-cells could be reisolated from AD I-treated animals by recruitment to the peritoneal cavity via Ag-specific challenge at this anatomical site. Reisolated memory PEC (mPEC) retained their reactivity and conferred tumour protection even after multiple transfers to subsequent tumour bearing nude mice.<br></br><br></br>The present model proved to be a valuable tool for the evaluation of the factors and mechanisms involved in the long-term maintenance of T-cell memory, and promises to yield further invaluable data in this field of research.","abstract_html":"An adoptive immunotherapy (ADI) protocol was developed where the fate and requirements of longterm persisting memory T-cells can be monitored. Anti-tumour immune peritoneal exudate cells (iPEC) were produced by injecting a subtumorigenic dose of the highly metastatic, ß-gal&lt;sup&gt;+&lt;/sup&gt; T-Iymphoma into the ear pinna of syngeneic DBN2 mice, followed by an intraperitoneal challenge with irradiation-inactivated tumour cells. 33.9 % of CD8&lt;sup&gt;+&lt;/sup&gt; iPEC were shown to be recognising the immunodominant peptide of ß-gal (aa 876-884), and, consistently, the iPEC exerted specific lysis of ß-gal&lt;sup&gt;+&lt;/sup&gt; cells. Upon adoptive transfer into sublethally irradiated, ESbL-Gal tumour-bearing, athymic Balb/c nu/nu mice, they conferred protective and long-lasting anti-tumour immunity. ADI-treated animals were able to reject subsequent high dose tumour challenges, and memory T-cells appeared to be only partially affected by y-irradiation. ß-gaI&lt;sup&gt;876-884&lt;/sup&gt; peptide/MHC class I tetramer stainings identified the bone marrow as the major compartment for the long-term persistence of memory T-cells, as ß-gaI&lt;sup&gt;876-884&lt;/sup&gt; specific T -cells occurred at elevated frequencies in this microenvironment as compared to the spleen and lymph nodes. In a &quot;parking experiment&quot;, Agremoval led to a decrease of tetramer-binding cells below background levels. Tumour-reactive memory T-cells could be reisolated from AD I-treated animals by recruitment to the peritoneal cavity via Ag-specific challenge at this anatomical site. Reisolated memory PEC (mPEC) retained their reactivity and conferred tumour protection even after multiple transfers to subsequent tumour bearing nude mice.&lt;br&gt;&lt;/br&gt;&lt;br&gt;&lt;/br&gt;The present model proved to be a valuable tool for the evaluation of the factors and mechanisms involved in the long-term maintenance of T-cell memory, and promises to yield further invaluable data in this field of research.","abstract_has_math":false,"creators":["Mahnke, Yolanda Dagmar"],"institution":"The Open University","degree_name":"phd","degree_level":"doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2001,"date_issued":"2001","date_published":"2001","updated_at":"2026-07-24T05:02:35Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:creator","label":"Author","values":["Mahnke, Yolanda Dagmar"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2001"]},{"key":"dc:date.issued","label":"Date","values":["2001"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["The Open University"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://oro.open.ac.uk/52284/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["phd"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://oro.open.ac.uk/52284/1/52284.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["An adoptive immunotherapy (ADI) protocol was developed where the fate and requirements of longterm persisting memory T-cells can be monitored. Anti-tumour immune peritoneal exudate cells (iPEC) were produced by injecting a subtumorigenic dose of the highly metastatic, ß-gal<sup>+</sup> T-Iymphoma into the ear pinna of syngeneic DBN2 mice, followed by an intraperitoneal challenge with irradiation-inactivated tumour cells. 33.9 % of CD8<sup>+</sup> iPEC were shown to be recognising the immunodominant peptide of ß-gal (aa 876-884), and, consistently, the iPEC exerted specific lysis of ß-gal<sup>+</sup> cells. Upon adoptive transfer into sublethally irradiated, ESbL-Gal tumour-bearing, athymic Balb/c nu/nu mice, they conferred protective and long-lasting anti-tumour immunity. ADI-treated animals were able to reject subsequent high dose tumour challenges, and memory T-cells appeared to be only partially affected by y-irradiation. ß-gaI<sup>876-884</sup> peptide/MHC class I tetramer stainings identified the bone marrow as the major compartment for the long-term persistence of memory T-cells, as ß-gaI<sup>876-884</sup> specific T -cells occurred at elevated frequencies in this microenvironment as compared to the spleen and lymph nodes. In a \"parking experiment\", Agremoval led to a decrease of tetramer-binding cells below background levels. Tumour-reactive memory T-cells could be reisolated from AD I-treated animals by recruitment to the peritoneal cavity via Ag-specific challenge at this anatomical site. Reisolated memory PEC (mPEC) retained their reactivity and conferred tumour protection even after multiple transfers to subsequent tumour bearing nude mice.<br></br><br></br>The present model proved to be a valuable tool for the evaluation of the factors and mechanisms involved in the long-term maintenance of T-cell memory, and promises to yield further invaluable data in this field of research."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["A novel model system for the study of anti-tumour T-cell memory"]}]}],"canonical_facts":{"dc:creator":["Mahnke, Yolanda Dagmar"],"dc:date":["2001"],"dc:date.issued":["2001"],"dc:description.abstract":["An adoptive immunotherapy (ADI) protocol was developed where the fate and requirements of longterm persisting memory T-cells can be monitored. Anti-tumour immune peritoneal exudate cells (iPEC) were produced by injecting a subtumorigenic dose of the highly metastatic, ß-gal<sup>+</sup> T-Iymphoma into the ear pinna of syngeneic DBN2 mice, followed by an intraperitoneal challenge with irradiation-inactivated tumour cells. 33.9 % of CD8<sup>+</sup> iPEC were shown to be recognising the immunodominant peptide of ß-gal (aa 876-884), and, consistently, the iPEC exerted specific lysis of ß-gal<sup>+</sup> cells. Upon adoptive transfer into sublethally irradiated, ESbL-Gal tumour-bearing, athymic Balb/c nu/nu mice, they conferred protective and long-lasting anti-tumour immunity. ADI-treated animals were able to reject subsequent high dose tumour challenges, and memory T-cells appeared to be only partially affected by y-irradiation. ß-gaI<sup>876-884</sup> peptide/MHC class I tetramer stainings identified the bone marrow as the major compartment for the long-term persistence of memory T-cells, as ß-gaI<sup>876-884</sup> specific T -cells occurred at elevated frequencies in this microenvironment as compared to the spleen and lymph nodes. In a \"parking experiment\", Agremoval led to a decrease of tetramer-binding cells below background levels. Tumour-reactive memory T-cells could be reisolated from AD I-treated animals by recruitment to the peritoneal cavity via Ag-specific challenge at this anatomical site. Reisolated memory PEC (mPEC) retained their reactivity and conferred tumour protection even after multiple transfers to subsequent tumour bearing nude mice.<br></br><br></br>The present model proved to be a valuable tool for the evaluation of the factors and mechanisms involved in the long-term maintenance of T-cell memory, and promises to yield further invaluable data in this field of research."],"dc:format":["application/pdf"],"dc:identifier.uri":["https://oro.open.ac.uk/52284/1/52284.pdf"],"dc:language":["en"],"dc:publisher.institution":["The Open University"],"dc:relation.isreferencedby":["https://oro.open.ac.uk/52284/"],"dc:title":["A novel model system for the study of anti-tumour T-cell memory"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["doctoral"],"dc:type.qualificationname":["phd"]},"updated_at":"2026-07-24T05:02:35Z"}