{"id":{"repo_id":"texas","oai_identifier":"oai:repositories.lib.utexas.edu:2152/22180"},"canonical_url":"https://search.dev.ndltd.org/etd/texas/oai:repositories.lib.utexas.edu:2152/22180","repository":{"repo_id":"texas","name":"University of Texas","base_url":"https://repositories.lib.utexas.edu/server/oai/request"},"display":{"title":"Transposable prophage Mu exists as an independent chromosomal domain in E. coli","abstract":"The 4.6 Mb circular E. coli chromosome is compacted by segregation into 400-500 supercoiled domains, created by both active and passive mechanisms like transcription and DNA-binding proteins. We find that transposable prophage Mu, transcriptionally silent by definition, is organized into an independent domain as determined by the close proximity of Mu termini L and R separated by a 37 kb Mu genome. Cre-loxP recombination is used in this study in vivo and in vitro. Critical to formation/maintenance of the Mu 'domain' configuration are a strong gyrase site SGS at the center of Mu, the Mu L end, the MuB protein, and the E. coli nucleoid-associated proteins IHF, Fis and HU. The Mu domain was observed at two structurally different chromosomal locations, and was specific to the Mu prophage, i.e. was not observed for the [mathematical symbol] prophage. A model is proposed that by employing its cis-elements to create a domain barrier for segregation and compaction of its genome, the large selfish DNA element Mu profits from the transposition-ready arrangement of its ends, while simultaneously providing a fitness advantage to the host.","abstract_html":"The 4.6 Mb circular E. coli chromosome is compacted by segregation into 400-500 supercoiled domains, created by both active and passive mechanisms like transcription and DNA-binding proteins. We find that transposable prophage Mu, transcriptionally silent by definition, is organized into an independent domain as determined by the close proximity of Mu termini L and R separated by a 37 kb Mu genome. Cre-loxP recombination is used in this study in vivo and in vitro. Critical to formation/maintenance of the Mu 'domain' configuration are a strong gyrase site SGS at the center of Mu, the Mu L end, the MuB protein, and the E. coli nucleoid-associated proteins IHF, Fis and HU. The Mu domain was observed at two structurally different chromosomal locations, and was specific to the Mu prophage, i.e. was not observed for the [mathematical symbol] prophage. A model is proposed that by employing its cis-elements to create a domain barrier for segregation and compaction of its genome, the large selfish DNA element Mu profits from the transposition-ready arrangement of its ends, while simultaneously providing a fitness advantage to the host.","abstract_has_math":false,"creators":["Lou, Zheng, active 2012"],"institution":"The University of Texas at Austin","degree_name":"Doctor of Philosophy","degree_level":"Doctoral","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":[],"advisors":["Harshey, Rasika M."],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-08","date_published":"2012-08","updated_at":"2026-07-24T05:01:04Z","subjects":["Bacteriophage Mu","Bacterial chromosomal structure"],"languages":["en_US"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2152/22180","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Harshey, Rasika M."]},{"key":"dc:creator","label":"Author","values":["Lou, Zheng, active 2012"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2013-11-14T18:51:22Z"]},{"key":"dc:date.issued","label":"Date","values":["2012-08"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["The University of Texas at Austin"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Bacteriophage Mu","Bacterial chromosomal structure"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/2152/22180"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["text"]},{"key":"dc:description.abstract","label":"Abstract","values":["The 4.6 Mb circular E. coli chromosome is compacted by segregation into 400-500 supercoiled domains, created by both active and passive mechanisms like transcription and DNA-binding proteins. We find that transposable prophage Mu, transcriptionally silent by definition, is organized into an independent domain as determined by the close proximity of Mu termini L and R separated by a 37 kb Mu genome. Cre-loxP recombination is used in this study in vivo and in vitro. Critical to formation/maintenance of the Mu 'domain' configuration are a strong gyrase site SGS at the center of Mu, the Mu L end, the MuB protein, and the E. coli nucleoid-associated proteins IHF, Fis and HU. The Mu domain was observed at two structurally different chromosomal locations, and was specific to the Mu prophage, i.e. was not observed for the [mathematical symbol] prophage. A model is proposed that by employing its cis-elements to create a domain barrier for segregation and compaction of its genome, the large selfish DNA element Mu profits from the transposition-ready arrangement of its ends, while simultaneously providing a fitness advantage to the host."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Transposable prophage Mu exists as an independent chromosomal domain in E. coli"]}]}],"canonical_facts":{"dc:contributor.advisor":["Harshey, Rasika M."],"dc:creator":["Lou, Zheng, active 2012"],"dc:date.accessioned":["2013-11-14T18:51:22Z"],"dc:date.issued":["2012-08"],"dc:description":["text"],"dc:description.abstract":["The 4.6 Mb circular E. coli chromosome is compacted by segregation into 400-500 supercoiled domains, created by both active and passive mechanisms like transcription and DNA-binding proteins. We find that transposable prophage Mu, transcriptionally silent by definition, is organized into an independent domain as determined by the close proximity of Mu termini L and R separated by a 37 kb Mu genome. Cre-loxP recombination is used in this study in vivo and in vitro. Critical to formation/maintenance of the Mu 'domain' configuration are a strong gyrase site SGS at the center of Mu, the Mu L end, the MuB protein, and the E. coli nucleoid-associated proteins IHF, Fis and HU. The Mu domain was observed at two structurally different chromosomal locations, and was specific to the Mu prophage, i.e. was not observed for the [mathematical symbol] prophage. A model is proposed that by employing its cis-elements to create a domain barrier for segregation and compaction of its genome, the large selfish DNA element Mu profits from the transposition-ready arrangement of its ends, while simultaneously providing a fitness advantage to the host."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["http://hdl.handle.net/2152/22180"],"dc:language.iso":["en_US"],"dc:subject":["Bacteriophage Mu","Bacterial chromosomal structure"],"dc:title":["Transposable prophage Mu exists as an independent chromosomal domain in E. coli"],"thesis:degree_discipline":["Microbiology"],"thesis:degree_level":["Doctoral"],"thesis:degree_name":["Doctor of Philosophy"],"thesis:institution_name":["The University of Texas at Austin"]},"updated_at":"2026-07-24T05:01:04Z"}