{"id":{"repo_id":"texas","oai_identifier":"oai:repositories.lib.utexas.edu:2152/12197"},"canonical_url":"https://search.dev.ndltd.org/etd/texas/oai:repositories.lib.utexas.edu:2152/12197","repository":{"repo_id":"texas","name":"University of Texas","base_url":"https://repositories.lib.utexas.edu/server/oai/request"},"display":{"title":"Nucleic acid biosensors","abstract":"There is an ever increasing need to expand the realm of diagnostic capabilities. The need for accurate and reliable biological detection methods is crucial for genetic analysis, drug discovery, the monitoring of bacteria and viruses in food and water samples, as well as monitoring the environment for biothreat reagents. In the realm of diagnostics, molecular biology first expanded to include semiconductor manufacturing technology in the creation of a DNA biosensor array. Currently, biosensor arrays are being developed to utilizing complex sensors for the detection of multiple types of analytes. Important to the future applications for biosensor technology is the development of miniaturized systems that utilize sophisticated sensors. We have explored the use of platforms that offer a three-dimensional volume element, as well as sophisticated nucleic acid sensors. A flow cell based microsphere platform (electronic tongue), and a novel hydrogel platform based on shape recognition elements (MUFFINS), have been combined with nucleic acid sensors (ssDNA and aptamers) and fluorescence microscopy to create biosensors for the detection of DNA and proteins. As a step towards fulfilling the goal of developing a second generation of sophisticated sensors, signaling aptamers were engineered. Signaling aptamers are nucleic acid ligands (aptamers) that are capable of displaying changes in fluorescent signal upon binding to their cognate analyte. In addition, “affinity” microspheres were developed for the direct synthesis of microsphere bound nucleic acid sensors.","abstract_html":"There is an ever increasing need to expand the realm of diagnostic capabilities. The need for accurate and reliable biological detection methods is crucial for genetic analysis, drug discovery, the monitoring of bacteria and viruses in food and water samples, as well as monitoring the environment for biothreat reagents. In the realm of diagnostics, molecular biology first expanded to include semiconductor manufacturing technology in the creation of a DNA biosensor array. Currently, biosensor arrays are being developed to utilizing complex sensors for the detection of multiple types of analytes. Important to the future applications for biosensor technology is the development of miniaturized systems that utilize sophisticated sensors. We have explored the use of platforms that offer a three-dimensional volume element, as well as sophisticated nucleic acid sensors. A flow cell based microsphere platform (electronic tongue), and a novel hydrogel platform based on shape recognition elements (MUFFINS), have been combined with nucleic acid sensors (ssDNA and aptamers) and fluorescence microscopy to create biosensors for the detection of DNA and proteins. As a step towards fulfilling the goal of developing a second generation of sophisticated sensors, signaling aptamers were engineered. Signaling aptamers are nucleic acid ligands (aptamers) that are capable of displaying changes in fluorescent signal upon binding to their cognate analyte. In addition, “affinity” microspheres were developed for the direct synthesis of microsphere bound nucleic acid sensors.","abstract_has_math":false,"creators":["Kirby, Romy, 1972-"],"institution":"The University of Texas at Austin","degree_name":"Doctor of Philosophy","degree_level":"Doctoral","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":[],"advisors":["Ellington, Andrew D."],"committee_chairs":[],"committee_members":[],"year":2003,"date_issued":"2003-08","date_published":"2003-08","updated_at":"2026-07-24T05:01:20Z","subjects":["Biosensors"],"languages":["eng"],"rights":["Copyright is held by the author. Presentation of this material on the Libraries' web site by University Libraries, The University of Texas at Austin was made possible under a limited license grant from the author who has retained all copyrights in the works."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2152/12197","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Ellington, Andrew D."]},{"key":"dc:creator","label":"Author","values":["Kirby, Romy, 1972-"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2011-07-11T17:55:07Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2011-07-11T17:55:07Z"]},{"key":"dc:date.issued","label":"Date","values":["2003-08"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["The University of Texas at Austin"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biosensors"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright is held by the author. 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In the realm of diagnostics, molecular biology first expanded to include semiconductor manufacturing technology in the creation of a DNA biosensor array. Currently, biosensor arrays are being developed to utilizing complex sensors for the detection of multiple types of analytes. Important to the future applications for biosensor technology is the development of miniaturized systems that utilize sophisticated sensors. We have explored the use of platforms that offer a three-dimensional volume element, as well as sophisticated nucleic acid sensors. A flow cell based microsphere platform (electronic tongue), and a novel hydrogel platform based on shape recognition elements (MUFFINS), have been combined with nucleic acid sensors (ssDNA and aptamers) and fluorescence microscopy to create biosensors for the detection of DNA and proteins. As a step towards fulfilling the goal of developing a second generation of sophisticated sensors, signaling aptamers were engineered. Signaling aptamers are nucleic acid ligands (aptamers) that are capable of displaying changes in fluorescent signal upon binding to their cognate analyte. In addition, “affinity” microspheres were developed for the direct synthesis of microsphere bound nucleic acid sensors."]},{"key":"dc:format.medium","label":"Dc Format Medium","values":["electronic"]},{"key":"dc:title","label":"Title","values":["Nucleic acid biosensors"]}]}],"canonical_facts":{"dc:contributor.advisor":["Ellington, Andrew D."],"dc:creator":["Kirby, Romy, 1972-"],"dc:date.accessioned":["2011-07-11T17:55:07Z"],"dc:date.available":["2011-07-11T17:55:07Z"],"dc:date.issued":["2003-08"],"dc:description":["text"],"dc:description.abstract":["There is an ever increasing need to expand the realm of diagnostic capabilities. 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A flow cell based microsphere platform (electronic tongue), and a novel hydrogel platform based on shape recognition elements (MUFFINS), have been combined with nucleic acid sensors (ssDNA and aptamers) and fluorescence microscopy to create biosensors for the detection of DNA and proteins. As a step towards fulfilling the goal of developing a second generation of sophisticated sensors, signaling aptamers were engineered. Signaling aptamers are nucleic acid ligands (aptamers) that are capable of displaying changes in fluorescent signal upon binding to their cognate analyte. In addition, “affinity” microspheres were developed for the direct synthesis of microsphere bound nucleic acid sensors."],"dc:format.medium":["electronic"],"dc:identifier.uri":["http://hdl.handle.net/2152/12197"],"dc:language.iso":["eng"],"dc:rights":["Copyright is held by the author. Presentation of this material on the Libraries' web site by University Libraries, The University of Texas at Austin was made possible under a limited license grant from the author who has retained all copyrights in the works."],"dc:subject":["Biosensors"],"dc:title":["Nucleic acid biosensors"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Doctoral"],"thesis:degree_name":["Doctor of Philosophy"],"thesis:institution_name":["The University of Texas at Austin"]},"updated_at":"2026-07-24T05:01:20Z"}