{"id":{"repo_id":"texas","oai_identifier":"oai:repositories.lib.utexas.edu:2152/116611"},"canonical_url":"https://search.dev.ndltd.org/etd/texas/oai:repositories.lib.utexas.edu:2152/116611","repository":{"repo_id":"texas","name":"University of Texas","base_url":"https://repositories.lib.utexas.edu/server/oai/request"},"display":{"title":"Effects of a time-restricted high- or low-fat diet on lipid absorption","abstract":"Background: Chronic high-fat diets and time-restricted feeding have been shown to impact multiple cardiometabolic parameters. The purpose of this work was to investigate the effects of timed macronutrient intake on lipid absorption. Methods: Adult male FVB/N mice were randomly assigned to one of 6 feeding groups (n=45, 7-8 animals/group) and kept on a strict 12h light: 12h dark feeding schedule (lights on at 10pm (Zeitgeber Time - ZT0) and lights off at 10am (ZT12)). Food was made available continuously to mice in the control ad libitum low-fat (CALF) and control ad libitum high-fat (CAHF) feeding groups. Mice in the control low-fat (CLF) and control high-fat (CHF) groups received low fat or high fat food, respectively, exclusively during ZT 12-16 and ZT 20-24. Mice in the early high-fat (EHF) group received access to high-fat food during ZT 12-16 and low-fat food during ZT 20-24. Mice in the early low-fat (ELF) group received access to the low-fat food during ZT 12-16, and high-fat food during ZT 20-24. EHF and ELF groups did not have access to food during ZT 16-20. Rodent diet foods were 45% kcal from fat or 10% kcal from fat in high-fat groups and low-fat groups, respectively. After 12 weeks of study protocol, fecal samples were collected over a 24h period. Lipid extractions were performed to quantify fecal lipid content. Plasma samples were collected during mice sacrifice and triglyceride concentration was quantified. Results: There was a significant effect of feeding group (p=0.0016) as well as a significant effect of time on fecal lipid content (p=<0.05). Cosinor analysis revealed that feeding groups CALF, CLF, EHF, ELF showed significant rhythmicity for fecal lipid content, with no rhythmicity in fecal lipid content for either group exclusively eating a high fat diet (CAHF and CHF). There was a significant difference in plasma triglycerides between feeding groups (F (5,24) = 13.14, p = <0.05). A Scheffe post hoc test revealed that fasting plasma triglycerides showed a trend toward higher concentrations in groups fed low-fat food. Conclusion: Chronic high-fat diets resulted in greater fecal lipid content and lower fasting plasma triglycerides compared to low-fat diets","abstract_html":"Background: Chronic high-fat diets and time-restricted feeding have been shown to impact multiple cardiometabolic parameters. The purpose of this work was to investigate the effects of timed macronutrient intake on lipid absorption. Methods: Adult male FVB/N mice were randomly assigned to one of 6 feeding groups (n=45, 7-8 animals/group) and kept on a strict 12h light: 12h dark feeding schedule (lights on at 10pm (Zeitgeber Time - ZT0) and lights off at 10am (ZT12)). Food was made available continuously to mice in the control ad libitum low-fat (CALF) and control ad libitum high-fat (CAHF) feeding groups. Mice in the control low-fat (CLF) and control high-fat (CHF) groups received low fat or high fat food, respectively, exclusively during ZT 12-16 and ZT 20-24. Mice in the early high-fat (EHF) group received access to high-fat food during ZT 12-16 and low-fat food during ZT 20-24. Mice in the early low-fat (ELF) group received access to the low-fat food during ZT 12-16, and high-fat food during ZT 20-24. EHF and ELF groups did not have access to food during ZT 16-20. Rodent diet foods were 45% kcal from fat or 10% kcal from fat in high-fat groups and low-fat groups, respectively. After 12 weeks of study protocol, fecal samples were collected over a 24h period. Lipid extractions were performed to quantify fecal lipid content. Plasma samples were collected during mice sacrifice and triglyceride concentration was quantified. Results: There was a significant effect of feeding group (p=0.0016) as well as a significant effect of time on fecal lipid content (p=<0.05). Cosinor analysis revealed that feeding groups CALF, CLF, EHF, ELF showed significant rhythmicity for fecal lipid content, with no rhythmicity in fecal lipid content for either group exclusively eating a high fat diet (CAHF and CHF). There was a significant difference in plasma triglycerides between feeding groups (F (5,24) = 13.14, p = <0.05). A Scheffe post hoc test revealed that fasting plasma triglycerides showed a trend toward higher concentrations in groups fed low-fat food. Conclusion: Chronic high-fat diets resulted in greater fecal lipid content and lower fasting plasma triglycerides compared to low-fat diets","abstract_has_math":false,"creators":["Balderrama, Jesse, Jr."],"institution":"The University of Texas at Austin","degree_name":"Master of Science in Nutritional Sciences","degree_level":"Masters","degree_discipline":"Nutritional Sciences","degree_department":null,"school":null,"contributors":[],"advisors":["Bray, Molly S."],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-12-07","date_published":"2021-12-07","updated_at":"2026-07-24T05:00:54Z","subjects":["Time-restricted","High-fat","Low-fat","Diet","Mouse","Lipid absorption","Triglycerides","Circadian","Meal timing","Lipid extraction"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://dx.doi.org/10.26153/tsw/43506"],"render_values":[{"text":"http://dx.doi.org/10.26153/tsw/43506","href":"http://dx.doi.org/10.26153/tsw/43506","code":true}]}]},"links":{"outbound_url":"https://hdl.handle.net/2152/116611","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Bray, Molly S."]},{"key":"dc:creator","label":"Author","values":["Balderrama, Jesse, Jr."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2022-11-09T01:59:29Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2022-11-09T01:59:29Z"]},{"key":"dc:date.issued","label":"Date","values":["2021-12-07"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Nutritional Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Nutritional Sciences"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["The University of Texas at Austin"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Time-restricted","High-fat","Low-fat","Diet","Mouse","Lipid absorption","Triglycerides","Circadian","Meal timing","Lipid extraction"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/2152/116611","http://dx.doi.org/10.26153/tsw/43506"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Background: Chronic high-fat diets and time-restricted feeding have been shown to impact multiple cardiometabolic parameters. The purpose of this work was to investigate the effects of timed macronutrient intake on lipid absorption. Methods: Adult male FVB/N mice were randomly assigned to one of 6 feeding groups (n=45, 7-8 animals/group) and kept on a strict 12h light: 12h dark feeding schedule (lights on at 10pm (Zeitgeber Time - ZT0) and lights off at 10am (ZT12)). Food was made available continuously to mice in the control ad libitum low-fat (CALF) and control ad libitum high-fat (CAHF) feeding groups. Mice in the control low-fat (CLF) and control high-fat (CHF) groups received low fat or high fat food, respectively, exclusively during ZT 12-16 and ZT 20-24. Mice in the early high-fat (EHF) group received access to high-fat food during ZT 12-16 and low-fat food during ZT 20-24. Mice in the early low-fat (ELF) group received access to the low-fat food during ZT 12-16, and high-fat food during ZT 20-24. EHF and ELF groups did not have access to food during ZT 16-20. Rodent diet foods were 45% kcal from fat or 10% kcal from fat in high-fat groups and low-fat groups, respectively. After 12 weeks of study protocol, fecal samples were collected over a 24h period. Lipid extractions were performed to quantify fecal lipid content. Plasma samples were collected during mice sacrifice and triglyceride concentration was quantified. Results: There was a significant effect of feeding group (p=0.0016) as well as a significant effect of time on fecal lipid content (p=<0.05). Cosinor analysis revealed that feeding groups CALF, CLF, EHF, ELF showed significant rhythmicity for fecal lipid content, with no rhythmicity in fecal lipid content for either group exclusively eating a high fat diet (CAHF and CHF). There was a significant difference in plasma triglycerides between feeding groups (F (5,24) = 13.14, p = <0.05). A Scheffe post hoc test revealed that fasting plasma triglycerides showed a trend toward higher concentrations in groups fed low-fat food. Conclusion: Chronic high-fat diets resulted in greater fecal lipid content and lower fasting plasma triglycerides compared to low-fat diets"]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Effects of a time-restricted high- or low-fat diet on lipid absorption"]}]}],"canonical_facts":{"dc:contributor.advisor":["Bray, Molly S."],"dc:creator":["Balderrama, Jesse, Jr."],"dc:date.accessioned":["2022-11-09T01:59:29Z"],"dc:date.available":["2022-11-09T01:59:29Z"],"dc:date.issued":["2021-12-07"],"dc:description.abstract":["Background: Chronic high-fat diets and time-restricted feeding have been shown to impact multiple cardiometabolic parameters. The purpose of this work was to investigate the effects of timed macronutrient intake on lipid absorption. Methods: Adult male FVB/N mice were randomly assigned to one of 6 feeding groups (n=45, 7-8 animals/group) and kept on a strict 12h light: 12h dark feeding schedule (lights on at 10pm (Zeitgeber Time - ZT0) and lights off at 10am (ZT12)). Food was made available continuously to mice in the control ad libitum low-fat (CALF) and control ad libitum high-fat (CAHF) feeding groups. Mice in the control low-fat (CLF) and control high-fat (CHF) groups received low fat or high fat food, respectively, exclusively during ZT 12-16 and ZT 20-24. Mice in the early high-fat (EHF) group received access to high-fat food during ZT 12-16 and low-fat food during ZT 20-24. Mice in the early low-fat (ELF) group received access to the low-fat food during ZT 12-16, and high-fat food during ZT 20-24. EHF and ELF groups did not have access to food during ZT 16-20. Rodent diet foods were 45% kcal from fat or 10% kcal from fat in high-fat groups and low-fat groups, respectively. After 12 weeks of study protocol, fecal samples were collected over a 24h period. Lipid extractions were performed to quantify fecal lipid content. Plasma samples were collected during mice sacrifice and triglyceride concentration was quantified. Results: There was a significant effect of feeding group (p=0.0016) as well as a significant effect of time on fecal lipid content (p=<0.05). Cosinor analysis revealed that feeding groups CALF, CLF, EHF, ELF showed significant rhythmicity for fecal lipid content, with no rhythmicity in fecal lipid content for either group exclusively eating a high fat diet (CAHF and CHF). There was a significant difference in plasma triglycerides between feeding groups (F (5,24) = 13.14, p = <0.05). A Scheffe post hoc test revealed that fasting plasma triglycerides showed a trend toward higher concentrations in groups fed low-fat food. Conclusion: Chronic high-fat diets resulted in greater fecal lipid content and lower fasting plasma triglycerides compared to low-fat diets"],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["https://hdl.handle.net/2152/116611","http://dx.doi.org/10.26153/tsw/43506"],"dc:language.iso":["en"],"dc:subject":["Time-restricted","High-fat","Low-fat","Diet","Mouse","Lipid absorption","Triglycerides","Circadian","Meal timing","Lipid extraction"],"dc:title":["Effects of a time-restricted high- or low-fat diet on lipid absorption"],"dc:type":["Thesis"],"thesis:degree_discipline":["Nutritional Sciences"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science in Nutritional Sciences"],"thesis:institution_name":["The University of Texas at Austin"]},"updated_at":"2026-07-24T05:00:54Z"}