Identification and Quantification using HPLC-ES/MS of Four Pentacyclic Oxindole Alkaloids in Uncaria tomentosa Wild Type
type: CreativeWork
description: Uncaria tomentosa of the Rubiaceae family is a liana or woody vine that is found in Central and South America, which derives its nickname “Uña de Gato” or translated “Cats Claw” from its claw shaped thorns that resemble the claws of a cat. These thorns are used for the plant vine to climb and stretch to a height of 30 m. U. tomentosa naturally produces a range of secondary metabolites such as alkaloids, triterpenes, flavonoids, glycosides, and procyanidins. The metabolites in U. tomentosa are valuable in the treatment a number of diseases like bursitis, which is the inflammation of bursae (small sacs) of synovial fluid in the body, genital herpes, cancer, allergies, and those people infected with the HIV virus (4, 5). In the current research study we examine the concentrations of metabolites in the roots, leaves, and stems of U. tomentosa by establishing a method to accurately quantitate the concentrations of metabolites. In particular, we are interested in identifying and quantifying four principal pharmacologically active pentacyclic oxindole alkaloids: Mitraphylline, Isomitraphylline, Uncaria C (pteropodine), and Uncaria E (isopteropodine. In order to optimize growth conditions, media with the essential composition of nutrients were established for growth of the wild type. U. tomentosa wild type plants were grown in different media including Murashige and Skoog (MSO), Vi Murashige and Skoog (!4 MS), Driver and Kuniyuki Walnut (DKW), Woody Plant Medium (WPM), Gamborg B5 medium (B5), Nitsch & Nitsch (N/N), Nitsch & Nitsch Charcoal, Schenk and Hilderbrant (SH), WHITE’S, and Homeostatic Soil (HSO) to observe optimal growth conditions. The plants were harvested and sent for side by side analysis of pentacyclic oxindole alkaloid (POA) content. The coupling of High Performance Liquid Chromatography (HPLC) and Electrospray Ionization Mass Spectrometry (ESI/MS) was the analytical method of choice because of its selectivity and sensitivity toward these POA’s (12, 13). We w…
abstract: Uncaria tomentosa of the Rubiaceae family is a liana or woody vine that is found in Central and South America, which derives its nickname “Uña de Gato” or translated “Cats Claw” from its claw shaped thorns that resemble the claws of a cat. These thorns are used for the plant vine to climb and stretch to a height of 30 m. U. tomentosa naturally produces a range of secondary metabolites such as alkaloids, triterpenes, flavonoids, glycosides, and procyanidins. The metabolites in U. tomentosa are valuable in the treatment a number of diseases like bursitis, which is the inflammation of bursae (small sacs) of synovial fluid in the body, genital herpes, cancer, allergies, and those people infected with the HIV virus (4, 5). In the current research study we examine the concentrations of metabolites in the roots, leaves, and stems of U. tomentosa by establishing a method to accurately quantitate the concentrations of metabolites. In particular, we are interested in identifying and quantifying four principal pharmacologically active pentacyclic oxindole alkaloids: Mitraphylline, Isomitraphylline, Uncaria C (pteropodine), and Uncaria E (isopteropodine. In order to optimize growth conditions, media with the essential composition of nutrients were established for growth of the wild type. U. tomentosa wild type plants were grown in different media including Murashige and Skoog (MSO), Vi Murashige and Skoog (!4 MS), Driver and Kuniyuki Walnut (DKW), Woody Plant Medium (WPM), Gamborg B5 medium (B5), Nitsch & Nitsch (N/N), Nitsch & Nitsch Charcoal, Schenk and Hilderbrant (SH), WHITE’S, and Homeostatic Soil (HSO) to observe optimal growth conditions. The plants were harvested and sent for side by side analysis of pentacyclic oxindole alkaloid (POA) content. The coupling of High Performance Liquid Chromatography (HPLC) and Electrospray Ionization Mass Spectrometry (ESI/MS) was the analytical method of choice because of its selectivity and sensitivity toward these POA’s (12, 13). We w…
author: Sauls, Daniel S.
language: en
keywords: Uncaria tomentosa, metabolites, alkaloids
date published: 2010-04
date modified: 2026-07-27T21:22:39Z
provider: Texas State University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Texas State University-San Marcos

PropertyValue
property ID: Handle
value: https://hdl.handle.net/10877/12510