Altered Vulvar Microbiomes in Vulvar Lichen Sclerosus
type: CreativeWork
description: BACKGROUND: Vulvar lichen sclerosus (VLS) is a chronic and progressive dermatosis that most commonly affects the anogenital region of postmenopausal women. VLS etiology is incompletely understood, though a role for immune system dysregulation has been suggested given associations with autoimmune disease. The skin microbiome plays an important role in cutaneous immune function and may contribute to VLS pathogenesis or expression. OBJECTIVE: In this prospective, longitudinal study, we investigated the association between bacterial and fungal microbiomes at the vulva and vagina in patients with VLS. METHODS: First, we recruited postmenopausal women with a clinical diagnosis of VLS and healthy postmenopausal controls from the same general population. Experimental samples were obtained from the clitoral hood, interlabial sulcus, labia minora, perineum, and vagina. Additionally, patients with VLS were re-sampled 10-14 weeks after once-daily treatment with ultrapotent topical corticosteroid. Negative control samples of the clinical sampling environment were obtained with each encounter. Microbial DNA was extracted from samples. Bacterial and fungal libraries were prepared with primer sets targeting the 16S V1-V3 and ITS1 regions respectively. These libraries were sequenced at the UT Southwestern Genomics Core. QIIME2 with a divisive amplicon denoising algorithm 2 (DADA2) pipeline was used to process and analyze sequences. Shannon index was used to quantify alpha diversity. Unweighted UniFrac (bacteria) and Bray-Curtis dissimilarity (fungi) were used to quantify beta diversity. Relative abundance testing was performed at sites with significant community structural alterations using Linear discriminant analysis Effect Size (LEFSE) based on disease status. Pairwise difference and distance comparisons were used to evaluate changes in bacterial alpha and beta diversity with treatment. A supervised learning classifier was used to determine whether disease status could be predict…
abstract: BACKGROUND: Vulvar lichen sclerosus (VLS) is a chronic and progressive dermatosis that most commonly affects the anogenital region of postmenopausal women. VLS etiology is incompletely understood, though a role for immune system dysregulation has been suggested given associations with autoimmune disease. The skin microbiome plays an important role in cutaneous immune function and may contribute to VLS pathogenesis or expression. OBJECTIVE: In this prospective, longitudinal study, we investigated the association between bacterial and fungal microbiomes at the vulva and vagina in patients with VLS. METHODS: First, we recruited postmenopausal women with a clinical diagnosis of VLS and healthy postmenopausal controls from the same general population. Experimental samples were obtained from the clitoral hood, interlabial sulcus, labia minora, perineum, and vagina. Additionally, patients with VLS were re-sampled 10-14 weeks after once-daily treatment with ultrapotent topical corticosteroid. Negative control samples of the clinical sampling environment were obtained with each encounter. Microbial DNA was extracted from samples. Bacterial and fungal libraries were prepared with primer sets targeting the 16S V1-V3 and ITS1 regions respectively. These libraries were sequenced at the UT Southwestern Genomics Core. QIIME2 with a divisive amplicon denoising algorithm 2 (DADA2) pipeline was used to process and analyze sequences. Shannon index was used to quantify alpha diversity. Unweighted UniFrac (bacteria) and Bray-Curtis dissimilarity (fungi) were used to quantify beta diversity. Relative abundance testing was performed at sites with significant community structural alterations using Linear discriminant analysis Effect Size (LEFSE) based on disease status. Pairwise difference and distance comparisons were used to evaluate changes in bacterial alpha and beta diversity with treatment. A supervised learning classifier was used to determine whether disease status could be predict…
author: Pyle, Hunter James
language: en
keywords: Vulvar Lichen Sclerosus, Microbiota
date published: 2023-05
date modified: 2026-07-27T21:19:08Z
provider: Texas Digital Library
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Texas Digital Library

PropertyValue
property ID: Handle
value: https://hdl.handle.net/2152.5/10625