{"id":{"repo_id":"tdl","oai_identifier":"oai:tdl-ir.tdl.org:20.500.12503/28358"},"canonical_url":"https://search.dev.ndltd.org/etd/tdl/oai:tdl-ir.tdl.org:20.500.12503/28358","repository":{"repo_id":"tdl","name":"Texas Digital Library","base_url":"https://tdl-ir.tdl.org/server/oai/request"},"display":{"title":"Characterization of a Novel Receptor CS1 in Human Lymphocytes; Studies in Natural Killer Cells and B-Lymphocytes","abstract":"The purpose of this study was to investigate the roles of CS1 on human lymphocytes. The molecular and functional characterization of CS1 receptor in the natural killer (NK) cells and B lymphocytes was investigated. CS1 (CRACC, novel Ly9) is a novel member of the CD2 family receptor expressed on natural killer (NK), T cells, activated Bcells and dendritic cells. To examine the existence of isoform of CS1, library from NK cells was screened based on wild type of CS1 (CS1-L). A splice variant form of CS1 (CS1-S), which lacks immunoreceptor tyrosine-based switch motifs (ITSMs) in cytoplasmic domain, was identified. To demonstrate the function of CS1 on human NK cells, transfectants that stably express each isoform were generated. CS1-L was able to mediate redirect cytotoxicity of P815 target cells as well as intracellular calcium influx in the presence of monoclonal antibody against CS1 suggesting that CS1-L is an activating receptor. CS1-S showed no effect on the cytolytic function and calcium influx suggesting that CS1-L and CS1-S may differentially regulate human NK cell functions. Although CS1 was also cloned from cDNA library of human B-lymphocytes as well as of NK cells, very little is known regarding its biology on human B-lymphocytes. Here I investigated the expressions and functions of CS1 in human B cells. Human B cells expresses only CS1-L isoform and the levels of CS1 expression are upregulated after activation in vitro. Importantly, monoclonal antibody of CS1 (1G10 mAb) strongly enhances proliferation of both freshly isolated and activated B cells. The enhanced proliferation effects of CS1 were most prominent on B cells activated by anti-CD40 mAbs and/or IL-4. Human cytokine microarray results indicated CS1 enhanced mRNA transcripts of fms-line tyrosine kinase 3 ligand, lymphotoxin A, tumor necrosis factor, and IL-14 which are related with mostly growth promoting activity. These results suggest that autorine cytokines might be the mediators for the function of CS1 on B cell in which it can induce proliferation of activated B cells. This study suggests that CS1 plays important role in human NK cells and B-lymphocytes.","abstract_html":"The purpose of this study was to investigate the roles of CS1 on human lymphocytes. The molecular and functional characterization of CS1 receptor in the natural killer (NK) cells and B lymphocytes was investigated. CS1 (CRACC, novel Ly9) is a novel member of the CD2 family receptor expressed on natural killer (NK), T cells, activated Bcells and dendritic cells. To examine the existence of isoform of CS1, library from NK cells was screened based on wild type of CS1 (CS1-L). A splice variant form of CS1 (CS1-S), which lacks immunoreceptor tyrosine-based switch motifs (ITSMs) in cytoplasmic domain, was identified. To demonstrate the function of CS1 on human NK cells, transfectants that stably express each isoform were generated. CS1-L was able to mediate redirect cytotoxicity of P815 target cells as well as intracellular calcium influx in the presence of monoclonal antibody against CS1 suggesting that CS1-L is an activating receptor. CS1-S showed no effect on the cytolytic function and calcium influx suggesting that CS1-L and CS1-S may differentially regulate human NK cell functions. Although CS1 was also cloned from cDNA library of human B-lymphocytes as well as of NK cells, very little is known regarding its biology on human B-lymphocytes. Here I investigated the expressions and functions of CS1 in human B cells. Human B cells expresses only CS1-L isoform and the levels of CS1 expression are upregulated after activation in vitro. Importantly, monoclonal antibody of CS1 (1G10 mAb) strongly enhances proliferation of both freshly isolated and activated B cells. The enhanced proliferation effects of CS1 were most prominent on B cells activated by anti-CD40 mAbs and/or IL-4. Human cytokine microarray results indicated CS1 enhanced mRNA transcripts of fms-line tyrosine kinase 3 ligand, lymphotoxin A, tumor necrosis factor, and IL-14 which are related with mostly growth promoting activity. These results suggest that autorine cytokines might be the mediators for the function of CS1 on B cell in which it can induce proliferation of activated B cells. This study suggests that CS1 plays important role in human NK cells and B-lymphocytes.","abstract_has_math":false,"creators":["Lee, Jae Kyung"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Mathew, Porunelloor","Wu, Ming-Chi","Das, Hriday"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2005,"date_issued":"2005-06-01","date_published":"2005-06-01","updated_at":"2026-07-27T21:19:01Z","subjects":["Cell Anatomy","Cell and Developmental Biology","Cell Biology","Cells","Immunology and Infectious Disease","Life Sciences","Medical Cell Biology","Medical Sciences","Medicine and Health Sciences","Other Cell and Developmental Biology","Other Immunology and Infectious Disease","CS1","human lymphocytes","natural killer cells","cell apoptosis","immunoreceptor tyrosine-based switch motifs","cytoplasmic domain","B cells","autorine cytokines"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.12503/28358","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Mathew, Porunelloor","Wu, Ming-Chi","Das, Hriday"]},{"key":"dc:creator","label":"Author","values":["Lee, Jae Kyung"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2019-08-22T20:25:53Z","2026-03-24T17:21:26Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2019-08-22T20:25:53Z"]},{"key":"dc:date.issued","label":"Date","values":["2005-06-01"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Cell Anatomy","Cell and Developmental Biology","Cell Biology","Cells","Immunology and Infectious Disease","Life Sciences","Medical Cell Biology","Medical Sciences","Medicine and Health Sciences","Other Cell and Developmental Biology","Other Immunology and Infectious Disease","CS1","human lymphocytes","natural killer cells","cell apoptosis","immunoreceptor tyrosine-based switch motifs","cytoplasmic domain","B cells","autorine cytokines"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/20.500.12503/28358"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.12503/28358"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The purpose of this study was to investigate the roles of CS1 on human lymphocytes. The molecular and functional characterization of CS1 receptor in the natural killer (NK) cells and B lymphocytes was investigated. CS1 (CRACC, novel Ly9) is a novel member of the CD2 family receptor expressed on natural killer (NK), T cells, activated Bcells and dendritic cells. To examine the existence of isoform of CS1, library from NK cells was screened based on wild type of CS1 (CS1-L). A splice variant form of CS1 (CS1-S), which lacks immunoreceptor tyrosine-based switch motifs (ITSMs) in cytoplasmic domain, was identified. To demonstrate the function of CS1 on human NK cells, transfectants that stably express each isoform were generated. CS1-L was able to mediate redirect cytotoxicity of P815 target cells as well as intracellular calcium influx in the presence of monoclonal antibody against CS1 suggesting that CS1-L is an activating receptor. CS1-S showed no effect on the cytolytic function and calcium influx suggesting that CS1-L and CS1-S may differentially regulate human NK cell functions. Although CS1 was also cloned from cDNA library of human B-lymphocytes as well as of NK cells, very little is known regarding its biology on human B-lymphocytes. Here I investigated the expressions and functions of CS1 in human B cells. Human B cells expresses only CS1-L isoform and the levels of CS1 expression are upregulated after activation in vitro. Importantly, monoclonal antibody of CS1 (1G10 mAb) strongly enhances proliferation of both freshly isolated and activated B cells. The enhanced proliferation effects of CS1 were most prominent on B cells activated by anti-CD40 mAbs and/or IL-4. Human cytokine microarray results indicated CS1 enhanced mRNA transcripts of fms-line tyrosine kinase 3 ligand, lymphotoxin A, tumor necrosis factor, and IL-14 which are related with mostly growth promoting activity. These results suggest that autorine cytokines might be the mediators for the function of CS1 on B cell in which it can induce proliferation of activated B cells. This study suggests that CS1 plays important role in human NK cells and B-lymphocytes."]},{"key":"dc:title","label":"Title","values":["Characterization of a Novel Receptor CS1 in Human Lymphocytes; Studies in Natural Killer Cells and B-Lymphocytes"]}]}],"canonical_facts":{"dc:contributor":["Mathew, Porunelloor","Wu, Ming-Chi","Das, Hriday"],"dc:creator":["Lee, Jae Kyung"],"dc:date.accessioned":["2019-08-22T20:25:53Z","2026-03-24T17:21:26Z"],"dc:date.available":["2019-08-22T20:25:53Z"],"dc:date.issued":["2005-06-01"],"dc:description.abstract":["The purpose of this study was to investigate the roles of CS1 on human lymphocytes. The molecular and functional characterization of CS1 receptor in the natural killer (NK) cells and B lymphocytes was investigated. CS1 (CRACC, novel Ly9) is a novel member of the CD2 family receptor expressed on natural killer (NK), T cells, activated Bcells and dendritic cells. To examine the existence of isoform of CS1, library from NK cells was screened based on wild type of CS1 (CS1-L). A splice variant form of CS1 (CS1-S), which lacks immunoreceptor tyrosine-based switch motifs (ITSMs) in cytoplasmic domain, was identified. To demonstrate the function of CS1 on human NK cells, transfectants that stably express each isoform were generated. CS1-L was able to mediate redirect cytotoxicity of P815 target cells as well as intracellular calcium influx in the presence of monoclonal antibody against CS1 suggesting that CS1-L is an activating receptor. CS1-S showed no effect on the cytolytic function and calcium influx suggesting that CS1-L and CS1-S may differentially regulate human NK cell functions. Although CS1 was also cloned from cDNA library of human B-lymphocytes as well as of NK cells, very little is known regarding its biology on human B-lymphocytes. Here I investigated the expressions and functions of CS1 in human B cells. Human B cells expresses only CS1-L isoform and the levels of CS1 expression are upregulated after activation in vitro. Importantly, monoclonal antibody of CS1 (1G10 mAb) strongly enhances proliferation of both freshly isolated and activated B cells. The enhanced proliferation effects of CS1 were most prominent on B cells activated by anti-CD40 mAbs and/or IL-4. Human cytokine microarray results indicated CS1 enhanced mRNA transcripts of fms-line tyrosine kinase 3 ligand, lymphotoxin A, tumor necrosis factor, and IL-14 which are related with mostly growth promoting activity. These results suggest that autorine cytokines might be the mediators for the function of CS1 on B cell in which it can induce proliferation of activated B cells. This study suggests that CS1 plays important role in human NK cells and B-lymphocytes."],"dc:identifier":["https://hdl.handle.net/20.500.12503/28358"],"dc:identifier.uri":["https://hdl.handle.net/20.500.12503/28358"],"dc:language":["en"],"dc:subject":["Cell Anatomy","Cell and Developmental Biology","Cell Biology","Cells","Immunology and Infectious Disease","Life Sciences","Medical Cell Biology","Medical Sciences","Medicine and Health Sciences","Other Cell and Developmental Biology","Other Immunology and Infectious Disease","CS1","human lymphocytes","natural killer cells","cell apoptosis","immunoreceptor tyrosine-based switch motifs","cytoplasmic domain","B cells","autorine cytokines"],"dc:title":["Characterization of a Novel Receptor CS1 in Human Lymphocytes; Studies in Natural Killer Cells and B-Lymphocytes"],"dc:type":["Dissertation"]},"updated_at":"2026-07-27T21:19:01Z"}