{"id":{"repo_id":"tdl","oai_identifier":"oai:tdl-ir.tdl.org:10735.1/10027"},"canonical_url":"https://search.dev.ndltd.org/etd/tdl/oai:tdl-ir.tdl.org:10735.1/10027","repository":{"repo_id":"tdl","name":"Texas Digital Library","base_url":"https://tdl-ir.tdl.org/server/oai/request"},"display":{"title":"Utilization of TMV-TEMPO as an in Vivo MRI Sensor of ROS Production in Liver Inflammation and as a DNP Agent","abstract":"Magnetic resonance imaging (MRI) is a powerful tool that can noninvasively generate exquisite images of soft tissues in living subjects for medical diagnostics. This PhD dissertation details the i) utility of a nitroxyl-modified tobacco mosaic virus (TMV) as an in vivo MRI contrast agent for detection of reactive oxygen species (ROS) in murine model of liver inflammation and ii) its feasibility as an agent in MRI signal-enhancing dynamic nuclear polarization (DNP) technology. Chapter 1 entails discussion of the basics of magnetic resonance and hyperpolarization. Chapter 2 involves discussion of the identification and physical characterization of spontaneous mutation of TMV in a laboratory environment. Chapter 3 shows the successful utility of reduced TMV- TEMPO as an in vivo T2 -weighted MRI sensor of ROS in lipopolysaccharide (LPS)-induced liver inflammation in Balb/c mice. Chapter 4 details the 13 C DNP testing of TMV-TEMPO as a polarizing agent and the use of gadolinium coordination polymer GduDEP as a 13 C DNP signal enhancer. Finally, the overall conclusion and outlook of these projects are detailed in Chapter 5. Overall, this PhD dissertation ties up the experimental details and discussions of a new in vivo MRI biosensor for ROS at least in the pre-clinical level, along with some details on improving the 13 C DNP signals.","abstract_html":"Magnetic resonance imaging (MRI) is a powerful tool that can noninvasively generate exquisite images of soft tissues in living subjects for medical diagnostics. This PhD dissertation details the i) utility of a nitroxyl-modified tobacco mosaic virus (TMV) as an in vivo MRI contrast agent for detection of reactive oxygen species (ROS) in murine model of liver inflammation and ii) its feasibility as an agent in MRI signal-enhancing dynamic nuclear polarization (DNP) technology. Chapter 1 entails discussion of the basics of magnetic resonance and hyperpolarization. Chapter 2 involves discussion of the identification and physical characterization of spontaneous mutation of TMV in a laboratory environment. Chapter 3 shows the successful utility of reduced TMV- TEMPO as an in vivo T2 -weighted MRI sensor of ROS in lipopolysaccharide (LPS)-induced liver inflammation in Balb/c mice. Chapter 4 details the 13 C DNP testing of TMV-TEMPO as a polarizing agent and the use of gadolinium coordination polymer GduDEP as a 13 C DNP signal enhancer. Finally, the overall conclusion and outlook of these projects are detailed in Chapter 5. Overall, this PhD dissertation ties up the experimental details and discussions of a new in vivo MRI biosensor for ROS at least in the pre-clinical level, along with some details on improving the 13 C DNP signals.","abstract_has_math":false,"creators":["Lumata, Jenica Laporca 1994-"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Gassensmith, Jeremiah J.","Sanchez, Erica L.","Lumata, Lloyd","Meloni, Gabriele","Smaldone, Ronald A.","Zheng, Jie"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"December 2023","date_published":"December 2023","updated_at":"2026-07-27T21:19:01Z","subjects":["Biochemistry","Liver inflammation","ROS","DNP","TMV","Superoxide","Bioconjugation","VLP","Magnetic resonance imaging"],"languages":["English"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/10735.1/10027","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gassensmith, Jeremiah J.","Sanchez, Erica L.","Lumata, Lloyd","Meloni, Gabriele","Smaldone, Ronald A.","Zheng, Jie"]},{"key":"dc:creator","label":"Author","values":["Lumata, Jenica Laporca 1994-"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2024-03-08T21:58:40Z","2026-06-05T22:47:36Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2024-03-08T21:58:40Z"]},{"key":"dc:date.issued","label":"Date","values":["December 2023"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biochemistry","Liver inflammation","ROS","DNP","TMV","Superoxide","Bioconjugation","VLP","Magnetic resonance imaging"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/10735.1/10027"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10735.1/10027"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Magnetic resonance imaging (MRI) is a powerful tool that can noninvasively generate exquisite images of soft tissues in living subjects for medical diagnostics. This PhD dissertation details the i) utility of a nitroxyl-modified tobacco mosaic virus (TMV) as an in vivo MRI contrast agent for detection of reactive oxygen species (ROS) in murine model of liver inflammation and ii) its feasibility as an agent in MRI signal-enhancing dynamic nuclear polarization (DNP) technology. Chapter 1 entails discussion of the basics of magnetic resonance and hyperpolarization. Chapter 2 involves discussion of the identification and physical characterization of spontaneous mutation of TMV in a laboratory environment. Chapter 3 shows the successful utility of reduced TMV- TEMPO as an in vivo T2 -weighted MRI sensor of ROS in lipopolysaccharide (LPS)-induced liver inflammation in Balb/c mice. Chapter 4 details the 13 C DNP testing of TMV-TEMPO as a polarizing agent and the use of gadolinium coordination polymer GduDEP as a 13 C DNP signal enhancer. Finally, the overall conclusion and outlook of these projects are detailed in Chapter 5. Overall, this PhD dissertation ties up the experimental details and discussions of a new in vivo MRI biosensor for ROS at least in the pre-clinical level, along with some details on improving the 13 C DNP signals."]},{"key":"dc:title","label":"Title","values":["Utilization of TMV-TEMPO as an in Vivo MRI Sensor of ROS Production in Liver Inflammation and as a DNP Agent"]}]}],"canonical_facts":{"dc:contributor":["Gassensmith, Jeremiah J.","Sanchez, Erica L.","Lumata, Lloyd","Meloni, Gabriele","Smaldone, Ronald A.","Zheng, Jie"],"dc:creator":["Lumata, Jenica Laporca 1994-"],"dc:date.accessioned":["2024-03-08T21:58:40Z","2026-06-05T22:47:36Z"],"dc:date.available":["2024-03-08T21:58:40Z"],"dc:date.issued":["December 2023"],"dc:description.abstract":["Magnetic resonance imaging (MRI) is a powerful tool that can noninvasively generate exquisite images of soft tissues in living subjects for medical diagnostics. This PhD dissertation details the i) utility of a nitroxyl-modified tobacco mosaic virus (TMV) as an in vivo MRI contrast agent for detection of reactive oxygen species (ROS) in murine model of liver inflammation and ii) its feasibility as an agent in MRI signal-enhancing dynamic nuclear polarization (DNP) technology. Chapter 1 entails discussion of the basics of magnetic resonance and hyperpolarization. Chapter 2 involves discussion of the identification and physical characterization of spontaneous mutation of TMV in a laboratory environment. Chapter 3 shows the successful utility of reduced TMV- TEMPO as an in vivo T2 -weighted MRI sensor of ROS in lipopolysaccharide (LPS)-induced liver inflammation in Balb/c mice. Chapter 4 details the 13 C DNP testing of TMV-TEMPO as a polarizing agent and the use of gadolinium coordination polymer GduDEP as a 13 C DNP signal enhancer. Finally, the overall conclusion and outlook of these projects are detailed in Chapter 5. Overall, this PhD dissertation ties up the experimental details and discussions of a new in vivo MRI biosensor for ROS at least in the pre-clinical level, along with some details on improving the 13 C DNP signals."],"dc:identifier":["https://hdl.handle.net/10735.1/10027"],"dc:identifier.uri":["https://hdl.handle.net/10735.1/10027"],"dc:language":["English"],"dc:subject":["Biochemistry","Liver inflammation","ROS","DNP","TMV","Superoxide","Bioconjugation","VLP","Magnetic resonance imaging"],"dc:title":["Utilization of TMV-TEMPO as an in Vivo MRI Sensor of ROS Production in Liver Inflammation and as a DNP Agent"],"dc:type":["Thesis"]},"updated_at":"2026-07-27T21:19:01Z"}