Phytochemical studies on natural products and their biological activities against Trypanosoma
type: CreativeWork
description: The therapeutic properties of plants have been recognised since the earliest human communities. One plant considered to have biological effects is Holy Basil (Ocimum sanctum L.), with pre-clinical screening uncovering many compounds with effects against bacteria, anaphylaxis, histamines, and diabetes as well as with wound healing and radio-protective effects, including eugenol, euginal, ursolic acid, carvacrol, linalool, limatrol, and caryophyllene. The phytochemistry of this plant is studied in the first section of this thesis.;Propolis is also receiving ample attention in the context of initiatives to discover novel drugs, thanks to its biological and pharmacological qualities. A resinous substance, propolis is produced by honeybees from different plant materials. Different biological effects have been allocated to propolis extracts by earlier studies and their phytoconstituents have been extensively characterised, especially flavonoids, polyphenols, phenolic aldehydes, sesquiterpene quinines, coumarins, amino acids, steroids and inorganic compounds.;The present study employed high-resolution LC-MS, HPLC-ELSD and NMR profiling to analyse the chemical composition of Holy Basil (Ocimum sanctum L.) and a range of types of propolis from various geographical areas. Subsequently, suitable methods of extraction, fractionation and identification were be employed to isolate and identify compounds. Furthermore, one-dimensional and two-dimensional NMR as well as LCMS will be applied to elucidate the structures of the isolated compounds.;A range of compounds were isolated from Holy Basil extracts and different types of propolis by employing various phytochemical methods, including VLC, CC, SEC, preparative TLC.;All crudes extracts, fractions as well as 12 isolated compounds then were subjected for biological testing against Trypanosoma, T. brucei S427 WT, and their cytotoxicity against mammalian cells was also determined. The Holy Basil MeOH extract yielded three pure compoun…
abstract: The therapeutic properties of plants have been recognised since the earliest human communities. One plant considered to have biological effects is Holy Basil (Ocimum sanctum L.), with pre-clinical screening uncovering many compounds with effects against bacteria, anaphylaxis, histamines, and diabetes as well as with wound healing and radio-protective effects, including eugenol, euginal, ursolic acid, carvacrol, linalool, limatrol, and caryophyllene. The phytochemistry of this plant is studied in the first section of this thesis.;Propolis is also receiving ample attention in the context of initiatives to discover novel drugs, thanks to its biological and pharmacological qualities. A resinous substance, propolis is produced by honeybees from different plant materials. Different biological effects have been allocated to propolis extracts by earlier studies and their phytoconstituents have been extensively characterised, especially flavonoids, polyphenols, phenolic aldehydes, sesquiterpene quinines, coumarins, amino acids, steroids and inorganic compounds.;The present study employed high-resolution LC-MS, HPLC-ELSD and NMR profiling to analyse the chemical composition of Holy Basil (Ocimum sanctum L.) and a range of types of propolis from various geographical areas. Subsequently, suitable methods of extraction, fractionation and identification were be employed to isolate and identify compounds. Furthermore, one-dimensional and two-dimensional NMR as well as LCMS will be applied to elucidate the structures of the isolated compounds.;A range of compounds were isolated from Holy Basil extracts and different types of propolis by employing various phytochemical methods, including VLC, CC, SEC, preparative TLC.;All crudes extracts, fractions as well as 12 isolated compounds then were subjected for biological testing against Trypanosoma, T. brucei S427 WT, and their cytotoxicity against mammalian cells was also determined. The Holy Basil MeOH extract yielded three pure compoun…
author: Alenzi, Naif
date published: 2018
date modified: 2026-07-24T04:48:24Z
provider: University of Strathclyde
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Strathclyde

PropertyValue
property ID: DOI
value: 10.48730/yfjw-8526