{"id":{"repo_id":"strathclyde","oai_identifier":"oai:strathclyde:9k41zd54n"},"canonical_url":"https://search.dev.ndltd.org/etd/strathclyde/oai:strathclyde:9k41zd54n","repository":{"repo_id":"strathclyde","name":"University of Strathclyde","base_url":"https://stax.strath.ac.uk/catalog/oai"},"display":{"title":"Novel intranasal GNRs-DNA vaccines against human papillomavirus infection","abstract":"Currently licensed HPV vaccines, Cervarix and Gardasil, have been successfulin preventing HPV infection and high-graded cervical intraepithelial neoplasia (CIN2,3) in school girls in the UK and other developed countries. However, their high costshave hindered their wider use in preventing HPV infection and CIN 2/3 associatedwith HPV type -16 and -18. This project aims to produce cheap and effective GNRsDNA vaccines. The vaccines were composed of two parts, gold nanorods and circularplasmid DNA that carries codon-optimized HPV type 16 L1 gene for expression ofthe viral capsid-like particles in host cells. GNRs were functionalized with either PEIor PDDAC cationic polymers for high affinity to negatively charged DNAs. TheDNA is constructed in the backbone of a mammalian expression vector, plasmidpcDNA3 YFP of which YFP offered a fluorescent indicator. The HPV type -16 L1was either expressed alone or together with E. coli heat labile toxin B subunit, a wellknown nasal adjuvant that enhances immunogenicity. In total, four GNRs-DNAwere produced: PEI-GNRs-pcDNA3 YFP L1 (PE-G-L), PEI-GNRs-pcDNA3 YFPL1-ELT (PE-G-EL), PDDAC-GNRs-pcDNA3 YFP L1 (PD-G-L), and PDDACGNRs-pcDNA3 YFP L1-ELT (PD-G-EL).The GNRs-DNA internalization led to expression of VLP in HEK293, HeLaand the murine bone marrow-derived macrophages cell. Intranasal immunization ofBALB/c mice with GNRs-DNA resulted in YFP expression in nasal and lung tissuesin one day. Three doses (3 × 480ng plasmid DNA per mouse) elicited neutralizingantibodies in the serum and antibody secreting cells in spleen lymphocytes specificto HPV16 L1 protein. PDG-YL appeared to be most potent and comparable topurified VLP protein in eliciting Nabs and ASCs.My work has demonstrated the feasibility of using GNRs-DNA as vaccineplatforms. GNRs-DNA is cheap to produce, easy to administer and stable at roomtemperature. These characteristics make GNRs-DNA attractive candidates for futureHPV vaccine development. The optimised concentration ratio of GNRs and DNA, Tcell immune-response, IgA secretion require further assessment before the clinicaltrial.","abstract_html":"Currently licensed HPV vaccines, Cervarix and Gardasil, have been successfulin preventing HPV infection and high-graded cervical intraepithelial neoplasia (CIN2,3) in school girls in the UK and other developed countries. However, their high costshave hindered their wider use in preventing HPV infection and CIN 2/3 associatedwith HPV type -16 and -18. This project aims to produce cheap and effective GNRsDNA vaccines. The vaccines were composed of two parts, gold nanorods and circularplasmid DNA that carries codon-optimized HPV type 16 L1 gene for expression ofthe viral capsid-like particles in host cells. GNRs were functionalized with either PEIor PDDAC cationic polymers for high affinity to negatively charged DNAs. TheDNA is constructed in the backbone of a mammalian expression vector, plasmidpcDNA3 YFP of which YFP offered a fluorescent indicator. The HPV type -16 L1was either expressed alone or together with E. coli heat labile toxin B subunit, a wellknown nasal adjuvant that enhances immunogenicity. In total, four GNRs-DNAwere produced: PEI-GNRs-pcDNA3 YFP L1 (PE-G-L), PEI-GNRs-pcDNA3 YFPL1-ELT (PE-G-EL), PDDAC-GNRs-pcDNA3 YFP L1 (PD-G-L), and PDDACGNRs-pcDNA3 YFP L1-ELT (PD-G-EL).The GNRs-DNA internalization led to expression of VLP in HEK293, HeLaand the murine bone marrow-derived macrophages cell. Intranasal immunization ofBALB/c mice with GNRs-DNA resulted in YFP expression in nasal and lung tissuesin one day. Three doses (3 × 480ng plasmid DNA per mouse) elicited neutralizingantibodies in the serum and antibody secreting cells in spleen lymphocytes specificto HPV16 L1 protein. PDG-YL appeared to be most potent and comparable topurified VLP protein in eliciting Nabs and ASCs.My work has demonstrated the feasibility of using GNRs-DNA as vaccineplatforms. GNRs-DNA is cheap to produce, easy to administer and stable at roomtemperature. These characteristics make GNRs-DNA attractive candidates for futureHPV vaccine development. The optimised concentration ratio of GNRs and DNA, Tcell immune-response, IgA secretion require further assessment before the clinicaltrial.","abstract_has_math":false,"creators":["Li, Wenqin"],"institution":"University of Strathclyde","degree_name":"phd","degree_level":"doctoral-pg","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Yu, Jun","Chen, Yu"],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015","date_published":"2015","updated_at":"2026-07-24T04:44:58Z","subjects":[],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier.doi","label":"DOI","values":["10.48730/n72q-w388"],"render_values":[{"text":"10.48730/n72q-w388","href":"https://doi.org/10.48730/n72q-w388","code":true}]},{"key":"dc:identifier","label":"Identifier","values":["T14765"],"render_values":[{"text":"T14765","href":null,"code":true}]},{"key":"dc:creator.authoridentifier","label":"Author Identifier","values":["201184117"],"render_values":[{"text":"201184117","href":null,"code":true}]}]},"links":{"outbound_url":"https://stax.strath.ac.uk/concern/theses/9k41zd54n","outbound_label":"Repository record","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Yu, Jun","Chen, Yu"]},{"key":"dc:creator","label":"Author","values":["Li, Wenqin"]},{"key":"dc:creator.authoridentifier","label":"Author Identifier","values":["201184117"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015"]},{"key":"dc:date.issued","label":"Date","values":["2015"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Strathclyde Institute of Pharmacy and Biomedical Sciences"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Strathclyde"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["doctoral-pg"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["phd"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["T14765"]},{"key":"dc:identifier.doi","label":"DOI","values":["10.48730/n72q-w388"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://stax.strath.ac.uk/concern/theses/9k41zd54n"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Previously held under moratorium from 21st November 2017 until 21st November 2022.","Currently licensed HPV vaccines, Cervarix and Gardasil, have been successfulin preventing HPV infection and high-graded cervical intraepithelial neoplasia (CIN2,3) in school girls in the UK and other developed countries. However, their high costshave hindered their wider use in preventing HPV infection and CIN 2/3 associatedwith HPV type -16 and -18. This project aims to produce cheap and effective GNRsDNA vaccines. The vaccines were composed of two parts, gold nanorods and circularplasmid DNA that carries codon-optimized HPV type 16 L1 gene for expression ofthe viral capsid-like particles in host cells. GNRs were functionalized with either PEIor PDDAC cationic polymers for high affinity to negatively charged DNAs. TheDNA is constructed in the backbone of a mammalian expression vector, plasmidpcDNA3 YFP of which YFP offered a fluorescent indicator. The HPV type -16 L1was either expressed alone or together with E. coli heat labile toxin B subunit, a wellknown nasal adjuvant that enhances immunogenicity. In total, four GNRs-DNAwere produced: PEI-GNRs-pcDNA3 YFP L1 (PE-G-L), PEI-GNRs-pcDNA3 YFPL1-ELT (PE-G-EL), PDDAC-GNRs-pcDNA3 YFP L1 (PD-G-L), and PDDACGNRs-pcDNA3 YFP L1-ELT (PD-G-EL).The GNRs-DNA internalization led to expression of VLP in HEK293, HeLaand the murine bone marrow-derived macrophages cell. Intranasal immunization ofBALB/c mice with GNRs-DNA resulted in YFP expression in nasal and lung tissuesin one day. Three doses (3 × 480ng plasmid DNA per mouse) elicited neutralizingantibodies in the serum and antibody secreting cells in spleen lymphocytes specificto HPV16 L1 protein. PDG-YL appeared to be most potent and comparable topurified VLP protein in eliciting Nabs and ASCs.My work has demonstrated the feasibility of using GNRs-DNA as vaccineplatforms. GNRs-DNA is cheap to produce, easy to administer and stable at roomtemperature. These characteristics make GNRs-DNA attractive candidates for futureHPV vaccine development. The optimised concentration ratio of GNRs and DNA, Tcell immune-response, IgA secretion require further assessment before the clinicaltrial."]},{"key":"dc:description.abstract","label":"Abstract","values":["Currently licensed HPV vaccines, Cervarix and Gardasil, have been successfulin preventing HPV infection and high-graded cervical intraepithelial neoplasia (CIN2,3) in school girls in the UK and other developed countries. However, their high costshave hindered their wider use in preventing HPV infection and CIN 2/3 associatedwith HPV type -16 and -18. This project aims to produce cheap and effective GNRsDNA vaccines. The vaccines were composed of two parts, gold nanorods and circularplasmid DNA that carries codon-optimized HPV type 16 L1 gene for expression ofthe viral capsid-like particles in host cells. GNRs were functionalized with either PEIor PDDAC cationic polymers for high affinity to negatively charged DNAs. TheDNA is constructed in the backbone of a mammalian expression vector, plasmidpcDNA3 YFP of which YFP offered a fluorescent indicator. The HPV type -16 L1was either expressed alone or together with E. coli heat labile toxin B subunit, a wellknown nasal adjuvant that enhances immunogenicity. In total, four GNRs-DNAwere produced: PEI-GNRs-pcDNA3 YFP L1 (PE-G-L), PEI-GNRs-pcDNA3 YFPL1-ELT (PE-G-EL), PDDAC-GNRs-pcDNA3 YFP L1 (PD-G-L), and PDDACGNRs-pcDNA3 YFP L1-ELT (PD-G-EL).The GNRs-DNA internalization led to expression of VLP in HEK293, HeLaand the murine bone marrow-derived macrophages cell. Intranasal immunization ofBALB/c mice with GNRs-DNA resulted in YFP expression in nasal and lung tissuesin one day. Three doses (3 × 480ng plasmid DNA per mouse) elicited neutralizingantibodies in the serum and antibody secreting cells in spleen lymphocytes specificto HPV16 L1 protein. PDG-YL appeared to be most potent and comparable topurified VLP protein in eliciting Nabs and ASCs.My work has demonstrated the feasibility of using GNRs-DNA as vaccineplatforms. GNRs-DNA is cheap to produce, easy to administer and stable at roomtemperature. These characteristics make GNRs-DNA attractive candidates for futureHPV vaccine development. The optimised concentration ratio of GNRs and DNA, Tcell immune-response, IgA secretion require further assessment before the clinicaltrial."]},{"key":"dc:title","label":"Title","values":["Novel intranasal GNRs-DNA vaccines against human papillomavirus infection"]}]}],"canonical_facts":{"dc:contributor.advisor":["Yu, Jun","Chen, Yu"],"dc:creator":["Li, Wenqin"],"dc:creator.authoridentifier":["201184117"],"dc:date":["2015"],"dc:date.issued":["2015"],"dc:description":["Previously held under moratorium from 21st November 2017 until 21st November 2022.","Currently licensed HPV vaccines, Cervarix and Gardasil, have been successfulin preventing HPV infection and high-graded cervical intraepithelial neoplasia (CIN2,3) in school girls in the UK and other developed countries. However, their high costshave hindered their wider use in preventing HPV infection and CIN 2/3 associatedwith HPV type -16 and -18. This project aims to produce cheap and effective GNRsDNA vaccines. The vaccines were composed of two parts, gold nanorods and circularplasmid DNA that carries codon-optimized HPV type 16 L1 gene for expression ofthe viral capsid-like particles in host cells. GNRs were functionalized with either PEIor PDDAC cationic polymers for high affinity to negatively charged DNAs. TheDNA is constructed in the backbone of a mammalian expression vector, plasmidpcDNA3 YFP of which YFP offered a fluorescent indicator. The HPV type -16 L1was either expressed alone or together with E. coli heat labile toxin B subunit, a wellknown nasal adjuvant that enhances immunogenicity. In total, four GNRs-DNAwere produced: PEI-GNRs-pcDNA3 YFP L1 (PE-G-L), PEI-GNRs-pcDNA3 YFPL1-ELT (PE-G-EL), PDDAC-GNRs-pcDNA3 YFP L1 (PD-G-L), and PDDACGNRs-pcDNA3 YFP L1-ELT (PD-G-EL).The GNRs-DNA internalization led to expression of VLP in HEK293, HeLaand the murine bone marrow-derived macrophages cell. Intranasal immunization ofBALB/c mice with GNRs-DNA resulted in YFP expression in nasal and lung tissuesin one day. Three doses (3 × 480ng plasmid DNA per mouse) elicited neutralizingantibodies in the serum and antibody secreting cells in spleen lymphocytes specificto HPV16 L1 protein. PDG-YL appeared to be most potent and comparable topurified VLP protein in eliciting Nabs and ASCs.My work has demonstrated the feasibility of using GNRs-DNA as vaccineplatforms. GNRs-DNA is cheap to produce, easy to administer and stable at roomtemperature. These characteristics make GNRs-DNA attractive candidates for futureHPV vaccine development. The optimised concentration ratio of GNRs and DNA, Tcell immune-response, IgA secretion require further assessment before the clinicaltrial."],"dc:description.abstract":["Currently licensed HPV vaccines, Cervarix and Gardasil, have been successfulin preventing HPV infection and high-graded cervical intraepithelial neoplasia (CIN2,3) in school girls in the UK and other developed countries. However, their high costshave hindered their wider use in preventing HPV infection and CIN 2/3 associatedwith HPV type -16 and -18. This project aims to produce cheap and effective GNRsDNA vaccines. The vaccines were composed of two parts, gold nanorods and circularplasmid DNA that carries codon-optimized HPV type 16 L1 gene for expression ofthe viral capsid-like particles in host cells. GNRs were functionalized with either PEIor PDDAC cationic polymers for high affinity to negatively charged DNAs. TheDNA is constructed in the backbone of a mammalian expression vector, plasmidpcDNA3 YFP of which YFP offered a fluorescent indicator. The HPV type -16 L1was either expressed alone or together with E. coli heat labile toxin B subunit, a wellknown nasal adjuvant that enhances immunogenicity. In total, four GNRs-DNAwere produced: PEI-GNRs-pcDNA3 YFP L1 (PE-G-L), PEI-GNRs-pcDNA3 YFPL1-ELT (PE-G-EL), PDDAC-GNRs-pcDNA3 YFP L1 (PD-G-L), and PDDACGNRs-pcDNA3 YFP L1-ELT (PD-G-EL).The GNRs-DNA internalization led to expression of VLP in HEK293, HeLaand the murine bone marrow-derived macrophages cell. Intranasal immunization ofBALB/c mice with GNRs-DNA resulted in YFP expression in nasal and lung tissuesin one day. Three doses (3 × 480ng plasmid DNA per mouse) elicited neutralizingantibodies in the serum and antibody secreting cells in spleen lymphocytes specificto HPV16 L1 protein. PDG-YL appeared to be most potent and comparable topurified VLP protein in eliciting Nabs and ASCs.My work has demonstrated the feasibility of using GNRs-DNA as vaccineplatforms. GNRs-DNA is cheap to produce, easy to administer and stable at roomtemperature. These characteristics make GNRs-DNA attractive candidates for futureHPV vaccine development. The optimised concentration ratio of GNRs and DNA, Tcell immune-response, IgA secretion require further assessment before the clinicaltrial."],"dc:identifier":["T14765"],"dc:identifier.doi":["10.48730/n72q-w388"],"dc:identifier.uri":["https://stax.strath.ac.uk/concern/theses/9k41zd54n"],"dc:publisher.department":["Strathclyde Institute of Pharmacy and Biomedical Sciences"],"dc:publisher.institution":["University of Strathclyde"],"dc:title":["Novel intranasal GNRs-DNA vaccines against human papillomavirus infection"],"dc:type.qualificationlevel":["doctoral-pg"],"dc:type.qualificationname":["phd"]},"updated_at":"2026-07-24T04:44:58Z"}