The COP9 Signalosome (CSN) is an evolutionarily conserved multi-protein complex that regulates proteasome-mediated proteolysis. While the CSN has been shown to function in various growth and developmental processes, the involvement of the CSN in the regulation of defense responses is largely unknown. It was shown that silencing of the CSN subunit CSN5 resulted in reduced wound-and herbivory-induced levels of jasmonic acid and reduced expression of wound-response genes. Unexpectedly, these plants also showed increased pathogenesis-related gene expression. Consequently, CSN5–silenced plants were more susceptible to feeding by herbivorous insects and infection by a necrotrophic fungus.
It has been shown previously in our lab that a MAP kinase signaling cascade is an essential part of systemin-mediated defense signaling and functions upstream of jasmonic acid biosynthesis. Co-silencing of MPK1/2 and CSN5 had a synergistic effect on pathogenesis-related gene expression, suggesting that they function in the same signaling network. Together these finding suggest a role for the CSN in regulating jasmonic acid-dependent plant defense responses.
","abstract_html":"<p>The COP9 Signalosome (CSN) is an evolutionarily conserved multi-protein complex that regulates proteasome-mediated proteolysis. While the CSN has been shown to function in various growth and developmental processes, the involvement of the CSN in the regulation of defense responses is largely unknown. It was shown that silencing of the CSN subunit <em>CSN5</em> resulted in reduced wound-and herbivory-induced levels of jasmonic acid and reduced expression of wound-response genes. Unexpectedly, these plants also showed increased pathogenesis-related gene expression. Consequently, <em>CSN5</em>–silenced plants were more susceptible to feeding by herbivorous insects and infection by a necrotrophic fungus.</p> <p>It has been shown previously in our lab that a MAP kinase signaling cascade is an essential part of systemin-mediated defense signaling and functions upstream of jasmonic acid biosynthesis. Co-silencing of <em>MPK1/2</em> and <em>CSN5</em> had a synergistic effect on pathogenesis-related gene expression, suggesting that they function in the same signaling network. Together these finding suggest a role for the CSN in regulating jasmonic acid-dependent plant defense responses.</p>","abstract_has_math":false,"creators":["Hind, Sarah Refi"],"institution":null,"degree_name":"Ph.D.","degree_level":"Campus Access Dissertation","degree_discipline":"Biological Sciences","degree_department":null,"school":null,"contributors":["Johannes W. Stratmann"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-01-01T08:00:00Z","date_published":"2010-01-01T08:00:00Z","updated_at":"2026-07-24T04:37:14Z","subjects":["Life Sciences","Medicine and Health Sciences","Physical Sciences and Mathematics","COP9 Signalosome","CSN","Defense Responses","MAPK"],"languages":[],"rights":["© 2010, Sarah Refi Hind"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarcommons.sc.edu/etd/187","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Johannes W. Stratmann"]},{"key":"dc:creator","label":"Author","values":["Hind, Sarah Refi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biological Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Campus Access Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Life Sciences","Medicine and Health Sciences","Physical Sciences and Mathematics","COP9 Signalosome","CSN","Defense Responses","MAPK"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© 2010, Sarah Refi Hind"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarcommons.sc.edu/etd/187"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The COP9 Signalosome (CSN) is an evolutionarily conserved multi-protein complex that regulates proteasome-mediated proteolysis. While the CSN has been shown to function in various growth and developmental processes, the involvement of the CSN in the regulation of defense responses is largely unknown. It was shown that silencing of the CSN subunit CSN5 resulted in reduced wound-and herbivory-induced levels of jasmonic acid and reduced expression of wound-response genes. Unexpectedly, these plants also showed increased pathogenesis-related gene expression. Consequently, CSN5–silenced plants were more susceptible to feeding by herbivorous insects and infection by a necrotrophic fungus.
It has been shown previously in our lab that a MAP kinase signaling cascade is an essential part of systemin-mediated defense signaling and functions upstream of jasmonic acid biosynthesis. Co-silencing of MPK1/2 and CSN5 had a synergistic effect on pathogenesis-related gene expression, suggesting that they function in the same signaling network. Together these finding suggest a role for the CSN in regulating jasmonic acid-dependent plant defense responses.
"]},{"key":"dc:title","label":"Title","values":["Role of the COP9 Signalosome In Defense Responses In tomato"]}]}],"canonical_facts":{"dc:contributor":["Johannes W. Stratmann"],"dc:creator":["Hind, Sarah Refi"],"dc:description.abstract":["The COP9 Signalosome (CSN) is an evolutionarily conserved multi-protein complex that regulates proteasome-mediated proteolysis. While the CSN has been shown to function in various growth and developmental processes, the involvement of the CSN in the regulation of defense responses is largely unknown. It was shown that silencing of the CSN subunit CSN5 resulted in reduced wound-and herbivory-induced levels of jasmonic acid and reduced expression of wound-response genes. Unexpectedly, these plants also showed increased pathogenesis-related gene expression. Consequently, CSN5–silenced plants were more susceptible to feeding by herbivorous insects and infection by a necrotrophic fungus.
It has been shown previously in our lab that a MAP kinase signaling cascade is an essential part of systemin-mediated defense signaling and functions upstream of jasmonic acid biosynthesis. Co-silencing of MPK1/2 and CSN5 had a synergistic effect on pathogenesis-related gene expression, suggesting that they function in the same signaling network. Together these finding suggest a role for the CSN in regulating jasmonic acid-dependent plant defense responses.
"],"dc:identifier":["https://scholarcommons.sc.edu/etd/187"],"dc:rights":["© 2010, Sarah Refi Hind"],"dc:subject":["Life Sciences","Medicine and Health Sciences","Physical Sciences and Mathematics","COP9 Signalosome","CSN","Defense Responses","MAPK"],"dc:title":["Role of the COP9 Signalosome In Defense Responses In tomato"],"thesis:degree_discipline":["Biological Sciences"],"thesis:degree_level":["Campus Access Dissertation"],"thesis:degree_name":["Ph.D."]},"updated_at":"2026-07-24T04:37:14Z"}