{"id":{"repo_id":"soton","oai_identifier":"oai:eprints.soton.ac.uk:173981"},"canonical_url":"https://search.dev.ndltd.org/etd/soton/oai:eprints.soton.ac.uk:173981","repository":{"repo_id":"soton","name":"University of Southampton","base_url":"https://eprints.soton.ac.uk/cgi/oai2"},"display":{"title":"Novel DNA probes for sensitive DNA detection","abstract":"The ability to detect and interrogate DNA sequences allows further understanding and<br/>diagnosis of genetic disease. The ability to perform such analysis of genetic material<br/>requires highly selective and reliable technologies. Furthermore techniques which can use<br/>simple and cheap equipment allow the use of such technologies for point of care analysis.<br/><br/>Described in this thesis are two novel DNA probe systems designed for mutation<br/>discrimination and sequence recognition of PCR products. A homogenous PCR system<br/>using HyBeacons® which utilise FRET to produce a three probe multiplex system and<br/>surface enhanced Raman detection method. Both of these systems allow multiplex<br/>detection of PCR products and mutation discrimination by melting temperature analysis.<br/>The research reported includes investigations into the effects of different modifications to<br/>improve the performance of HyBeacon® probes as well as the effect of different dyes in a<br/>FRET system, including unique changes in the optical properties of such dyes. Also a<br/>novel method of performing melting temperature analysis using an electrochemical<br/>potential is reported.<br/><br/>In addition to the detection methods described this thesis includes initial work into the<br/>stabilisation of quantum dot nanoparticles for their use in aqueous systems as a potential<br/>alternative to fluorescent organic molecules.","abstract_html":"The ability to detect and interrogate DNA sequences allows further understanding and&lt;br/&gt;diagnosis of genetic disease. The ability to perform such analysis of genetic material&lt;br/&gt;requires highly selective and reliable technologies. Furthermore techniques which can use&lt;br/&gt;simple and cheap equipment allow the use of such technologies for point of care analysis.&lt;br/&gt;&lt;br/&gt;Described in this thesis are two novel DNA probe systems designed for mutation&lt;br/&gt;discrimination and sequence recognition of PCR products. A homogenous PCR system&lt;br/&gt;using HyBeacons® which utilise FRET to produce a three probe multiplex system and&lt;br/&gt;surface enhanced Raman detection method. Both of these systems allow multiplex&lt;br/&gt;detection of PCR products and mutation discrimination by melting temperature analysis.&lt;br/&gt;The research reported includes investigations into the effects of different modifications to&lt;br/&gt;improve the performance of HyBeacon® probes as well as the effect of different dyes in a&lt;br/&gt;FRET system, including unique changes in the optical properties of such dyes. Also a&lt;br/&gt;novel method of performing melting temperature analysis using an electrochemical&lt;br/&gt;potential is reported.&lt;br/&gt;&lt;br/&gt;In addition to the detection methods described this thesis includes initial work into the&lt;br/&gt;stabilisation of quantum dot nanoparticles for their use in aqueous systems as a potential&lt;br/&gt;alternative to fluorescent organic molecules.","abstract_has_math":false,"creators":["Richardson, James Alistair"],"institution":"University of Southampton","degree_name":"Ph.D.","degree_level":"doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Brown, Tom"],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-03","date_published":"2010-03","updated_at":"2026-07-24T04:36:21Z","subjects":[],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Brown, Tom"]},{"key":"dc:creator","label":"Author","values":["Richardson, James Alistair"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2010-03-29"]},{"key":"dc:date.issued","label":"Date","values":["2010-03"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Chemistry (pre 2011 reorg)","School of Chemistry"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Southampton"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://eprints.soton.ac.uk/173981/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["Ph.D."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://eprints.soton.ac.uk/173981/1/JR_thesis_With_corrections_electron_e_thesis.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The ability to detect and interrogate DNA sequences allows further understanding and<br/>diagnosis of genetic disease. The ability to perform such analysis of genetic material<br/>requires highly selective and reliable technologies. Furthermore techniques which can use<br/>simple and cheap equipment allow the use of such technologies for point of care analysis.<br/><br/>Described in this thesis are two novel DNA probe systems designed for mutation<br/>discrimination and sequence recognition of PCR products. A homogenous PCR system<br/>using HyBeacons® which utilise FRET to produce a three probe multiplex system and<br/>surface enhanced Raman detection method. Both of these systems allow multiplex<br/>detection of PCR products and mutation discrimination by melting temperature analysis.<br/>The research reported includes investigations into the effects of different modifications to<br/>improve the performance of HyBeacon® probes as well as the effect of different dyes in a<br/>FRET system, including unique changes in the optical properties of such dyes. Also a<br/>novel method of performing melting temperature analysis using an electrochemical<br/>potential is reported.<br/><br/>In addition to the detection methods described this thesis includes initial work into the<br/>stabilisation of quantum dot nanoparticles for their use in aqueous systems as a potential<br/>alternative to fluorescent organic molecules."]},{"key":"dc:format","label":"Dc Format","values":["text"]},{"key":"dc:title","label":"Title","values":["Novel DNA probes for sensitive DNA detection"]}]}],"canonical_facts":{"dc:contributor.advisor":["Brown, Tom"],"dc:creator":["Richardson, James Alistair"],"dc:date":["2010-03-29"],"dc:date.issued":["2010-03"],"dc:description.abstract":["The ability to detect and interrogate DNA sequences allows further understanding and<br/>diagnosis of genetic disease. The ability to perform such analysis of genetic material<br/>requires highly selective and reliable technologies. Furthermore techniques which can use<br/>simple and cheap equipment allow the use of such technologies for point of care analysis.<br/><br/>Described in this thesis are two novel DNA probe systems designed for mutation<br/>discrimination and sequence recognition of PCR products. A homogenous PCR system<br/>using HyBeacons® which utilise FRET to produce a three probe multiplex system and<br/>surface enhanced Raman detection method. Both of these systems allow multiplex<br/>detection of PCR products and mutation discrimination by melting temperature analysis.<br/>The research reported includes investigations into the effects of different modifications to<br/>improve the performance of HyBeacon® probes as well as the effect of different dyes in a<br/>FRET system, including unique changes in the optical properties of such dyes. Also a<br/>novel method of performing melting temperature analysis using an electrochemical<br/>potential is reported.<br/><br/>In addition to the detection methods described this thesis includes initial work into the<br/>stabilisation of quantum dot nanoparticles for their use in aqueous systems as a potential<br/>alternative to fluorescent organic molecules."],"dc:format":["text"],"dc:identifier.uri":["https://eprints.soton.ac.uk/173981/1/JR_thesis_With_corrections_electron_e_thesis.pdf"],"dc:publisher.department":["Chemistry (pre 2011 reorg)","School of Chemistry"],"dc:publisher.institution":["University of Southampton"],"dc:relation.isreferencedby":["https://eprints.soton.ac.uk/173981/"],"dc:title":["Novel DNA probes for sensitive DNA detection"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["doctoral"],"dc:type.qualificationname":["Ph.D."]},"updated_at":"2026-07-24T04:36:21Z"}