Identification and Characterization of Phytoplankton in Barnegat Bay, New Jersey

<p>Barnegat Bay is a marine estuary that encompasses 670-square miles of the eastern coast of New Jersey. The bay serves as a home to thousands of species of plants, animals, and microorganisms. During the summer months, the population of the bay increases from 576,000 to over 1.5 million residents. In 2011, the Barnegat Bay Partnership reported that environmental conditions of the bay are declining due to increased pollutants from nutrient fertilizers and runoff. Increased nitrogen levels have led to eutrophication and proliferation of phytoplankton algal blooms. Harmful algal blooms (HABs) cause a decrease in dissolved oxygen levels in the water as well as the release of harmful toxins that affect organisms inhabiting the bay, including humans.</p> <p>To research and develop methods to prevent HABs from occurring, 16 Barnegat Bay sites ranging from the northern to southern regions of the bay were studied in order identify species of cyanobacteria, such as <em>Synechococcus </em>and <em>Prochlorococcus,</em> and other phytoplankton such as diatoms and dinoflagellates. Polymerase chain reaction (PCR), microscopy, and flow cytometry were utilized to generate profiles of each site. Collectively, the utilization of the three methods detected phytoplankton species such as <em>Synechococcus, Prochlorococcus, </em>and <em>Cylindrotheca fusiformis</em>. Viral plaque assays using viral lysate from each site detected the presence of cyanophage as a natural control for phytoplankton populations.</p>

Name thesis:degree_name

MS Microbiology

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biology

Year dc:date.available

2014

Author dc:creator

• Elia, Nicole L.

Contributors dc:contributor

• Tin-Chun Chu
• Carolyn S. Bentivegna
• Marian Glenn
• Allan Blake
• Jane Ko

• Barnegat Bay
• phytoplankton
• cyanobacteria
• algal bloom
• flow cytometry
• Synechococcus
• Environmental Microbiology and Microbial Ecology

Repository record dc:identifier

https://scholarship.shu.edu/dissertations/1977

OAI identifier oai:identifier

oai:scholarship.shu.edu:dissertations-2973