{"id":{"repo_id":"sherbrooke","oai_identifier":"oai:usherbrooke.scholaris.ca:11143/20599"},"canonical_url":"https://search.dev.ndltd.org/etd/sherbrooke/oai:usherbrooke.scholaris.ca:11143/20599","repository":{"repo_id":"sherbrooke","name":"Université de Sherbrooke","base_url":"https://usherbrooke.scholaris.ca/server/oai/request"},"display":{"title":"Human neutrophils generate extracellular vesicles that modulate their functional responses","abstract":"Neutrophils influence innate and adaptive immunity by releasing various cytokines and chemokines, by generating neutrophil extracellular traps (NETs), and by modulating their own survival. Neutrophils also produce extracellular vesicles (EVs) termed ectosomes, which influence the function of other immune cells. Here, we studied neutrophil-derived ectosomes (NDEs) and whether they can modulate autologous neutrophil responses. We first characterized EV production by neutrophils, following MISEV 2018 guidelines to facilitate comparisons with other studies. We found that such EVs are principally NDEs, that they are rapidly released in response to several (but not all) physiological stimuli, and that a number of signaling pathways are involved in the induction of this response. When co-incubated with autologous neutrophils, NDE constituents were rapidly incorporated into recipient cells and this triggered and/or modulated neutrophil responses. The pro-survival effect of GM-CSF, G-CSF, IFNγ, and dexamethasone was reversed; CXCL8 and NET formation were induced in otherwise unstimulated neutrophils; the induction of inflammatory chemokines by TNFα was modulated depending on the activation state of the NDEs’ parent cells; and inducible NET generation was attenuated. Our data show that NDE generation modulates neutrophil responses in an autocrine and paracrine manner and indicate that this probably represents an important aspect of how neutrophils shape their environment and cellular interactions.","abstract_html":"Neutrophils influence innate and adaptive immunity by releasing various cytokines and chemokines, by generating neutrophil extracellular traps (NETs), and by modulating their own survival. Neutrophils also produce extracellular vesicles (EVs) termed ectosomes, which influence the function of other immune cells. Here, we studied neutrophil-derived ectosomes (NDEs) and whether they can modulate autologous neutrophil responses. We first characterized EV production by neutrophils, following MISEV 2018 guidelines to facilitate comparisons with other studies. We found that such EVs are principally NDEs, that they are rapidly released in response to several (but not all) physiological stimuli, and that a number of signaling pathways are involved in the induction of this response. When co-incubated with autologous neutrophils, NDE constituents were rapidly incorporated into recipient cells and this triggered and/or modulated neutrophil responses. The pro-survival effect of GM-CSF, G-CSF, IFNγ, and dexamethasone was reversed; CXCL8 and NET formation were induced in otherwise unstimulated neutrophils; the induction of inflammatory chemokines by TNFα was modulated depending on the activation state of the NDEs’ parent cells; and inducible NET generation was attenuated. Our data show that NDE generation modulates neutrophil responses in an autocrine and paracrine manner and indicate that this probably represents an important aspect of how neutrophils shape their environment and cellular interactions.","abstract_has_math":false,"creators":["Hurtado Gutierrez, Maria José"],"institution":"Université de Sherbrooke","degree_name":"M. Sc.","degree_level":"Maîtrise","degree_discipline":"Immunologie","degree_department":null,"school":null,"contributors":[],"advisors":["McDonald, Patrick","Dubois, Claire"],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023","date_published":"2023","updated_at":"2026-07-27T21:07:47Z","subjects":["Neutrophils","Extracellular vesicles","Apoptosis","Chemokines","NETs","Autocrine","Vésicles extracellulaires"],"languages":["en"],"rights":[],"rights_urls":["http://creativecommons.org/licenses/by-nc-nd/2.5/ca/"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11143/20599","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["McDonald, Patrick","Dubois, Claire"]},{"key":"dc:creator","label":"Author","values":["Hurtado Gutierrez, Maria José"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2023-08-29T12:35:50Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2023-08-29T12:35:50Z"]},{"key":"dc:date.issued","label":"Date","values":["2023"]},{"key":"dc:publisher","label":"Institution","values":["Université de Sherbrooke"]},{"key":"dc:type","label":"Dc Type","values":["Mémoire de maîtrise"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Immunologie"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Maîtrise"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M. Sc."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Faculté de médecine et des sciences de la santé"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Neutrophils","Extracellular vesicles","Apoptosis","Chemokines","NETs","Autocrine","Vésicles extracellulaires"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]},{"key":"dc:rights.uri","label":"Rights URI","values":["http://creativecommons.org/licenses/by-nc-nd/2.5/ca/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11143/20599"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Neutrophils influence innate and adaptive immunity by releasing various cytokines and chemokines, by generating neutrophil extracellular traps (NETs), and by modulating their own survival. Neutrophils also produce extracellular vesicles (EVs) termed ectosomes, which influence the function of other immune cells. Here, we studied neutrophil-derived ectosomes (NDEs) and whether they can modulate autologous neutrophil responses. We first characterized EV production by neutrophils, following MISEV 2018 guidelines to facilitate comparisons with other studies. We found that such EVs are principally NDEs, that they are rapidly released in response to several (but not all) physiological stimuli, and that a number of signaling pathways are involved in the induction of this response. When co-incubated with autologous neutrophils, NDE constituents were rapidly incorporated into recipient cells and this triggered and/or modulated neutrophil responses. The pro-survival effect of GM-CSF, G-CSF, IFNγ, and dexamethasone was reversed; CXCL8 and NET formation were induced in otherwise unstimulated neutrophils; the induction of inflammatory chemokines by TNFα was modulated depending on the activation state of the NDEs’ parent cells; and inducible NET generation was attenuated. Our data show that NDE generation modulates neutrophil responses in an autocrine and paracrine manner and indicate that this probably represents an important aspect of how neutrophils shape their environment and cellular interactions.","Les neutrophiles influencent l'immunité innée et adaptative en libérant diverses cytokines et chimiokines, en générant des pièges extracellulaires (NETs) et en modulant leur propre survie. Les neutrophiles produisent également des vésicules extracellulaires (VE) appelées ectosomes, qui influencent la fonction d'autres cellules immunitaires. Nous avons étudié les ectosomes dérivés des neutrophiles (NDE) et cherché à savoir s'ils pouvaient moduler les réponses des neutrophiles autologues. Nous avons d'abord caractérisé la production d'EV par les neutrophiles, en suivant les lignes directrices de MISEV 2018 pour faciliter les comparaisons avec d'autres études. Nous avons constaté que ces EV sont principalement des NDE, qu'ils sont rapidement libérés en réponse à plusieurs stimuli physiologiques (mais pas tous) et qu'un certain nombre de voies de signalisation sont impliquées dans l'induction de cette réponse. Lorsqu'ils sont co-incubés avec des neutrophiles autologues, les constituants des NDE sont rapidement incorporés dans les cellules réceptrices, ce qui déclenche et/ou module les réponses des neutrophiles. L'effet pro-survie du GM-CSF, du G-CSF, de l'IFNγ et de la dexaméthasone a été ainsi supprimé ; la formation de CXCL8 et de NETs a été induite chez des neutrophiles non stimulés ; l'induction de chimiokines inflammatoires par le TNFα a été modulée en fonction de l'état d'activation des cellules d’origine des NDE; et l’induction de NETs a été atténuée. Nos données montrent que la génération de NDE module les réponses des neutrophiles de manière autocrine et paracrine, et indiquent que cela représente probablement un aspect important de la manière dont les neutrophiles façonnent leur environnement et leurs interactions cellulaires."]},{"key":"dc:title","label":"Title","values":["Human neutrophils generate extracellular vesicles that modulate their functional responses"]}]}],"canonical_facts":{"dc:contributor.advisor":["McDonald, Patrick","Dubois, Claire"],"dc:creator":["Hurtado Gutierrez, Maria José"],"dc:date.accessioned":["2023-08-29T12:35:50Z"],"dc:date.available":["2023-08-29T12:35:50Z"],"dc:date.issued":["2023"],"dc:description.abstract":["Neutrophils influence innate and adaptive immunity by releasing various cytokines and chemokines, by generating neutrophil extracellular traps (NETs), and by modulating their own survival. Neutrophils also produce extracellular vesicles (EVs) termed ectosomes, which influence the function of other immune cells. Here, we studied neutrophil-derived ectosomes (NDEs) and whether they can modulate autologous neutrophil responses. We first characterized EV production by neutrophils, following MISEV 2018 guidelines to facilitate comparisons with other studies. We found that such EVs are principally NDEs, that they are rapidly released in response to several (but not all) physiological stimuli, and that a number of signaling pathways are involved in the induction of this response. When co-incubated with autologous neutrophils, NDE constituents were rapidly incorporated into recipient cells and this triggered and/or modulated neutrophil responses. The pro-survival effect of GM-CSF, G-CSF, IFNγ, and dexamethasone was reversed; CXCL8 and NET formation were induced in otherwise unstimulated neutrophils; the induction of inflammatory chemokines by TNFα was modulated depending on the activation state of the NDEs’ parent cells; and inducible NET generation was attenuated. Our data show that NDE generation modulates neutrophil responses in an autocrine and paracrine manner and indicate that this probably represents an important aspect of how neutrophils shape their environment and cellular interactions.","Les neutrophiles influencent l'immunité innée et adaptative en libérant diverses cytokines et chimiokines, en générant des pièges extracellulaires (NETs) et en modulant leur propre survie. Les neutrophiles produisent également des vésicules extracellulaires (VE) appelées ectosomes, qui influencent la fonction d'autres cellules immunitaires. Nous avons étudié les ectosomes dérivés des neutrophiles (NDE) et cherché à savoir s'ils pouvaient moduler les réponses des neutrophiles autologues. Nous avons d'abord caractérisé la production d'EV par les neutrophiles, en suivant les lignes directrices de MISEV 2018 pour faciliter les comparaisons avec d'autres études. Nous avons constaté que ces EV sont principalement des NDE, qu'ils sont rapidement libérés en réponse à plusieurs stimuli physiologiques (mais pas tous) et qu'un certain nombre de voies de signalisation sont impliquées dans l'induction de cette réponse. Lorsqu'ils sont co-incubés avec des neutrophiles autologues, les constituants des NDE sont rapidement incorporés dans les cellules réceptrices, ce qui déclenche et/ou module les réponses des neutrophiles. L'effet pro-survie du GM-CSF, du G-CSF, de l'IFNγ et de la dexaméthasone a été ainsi supprimé ; la formation de CXCL8 et de NETs a été induite chez des neutrophiles non stimulés ; l'induction de chimiokines inflammatoires par le TNFα a été modulée en fonction de l'état d'activation des cellules d’origine des NDE; et l’induction de NETs a été atténuée. Nos données montrent que la génération de NDE module les réponses des neutrophiles de manière autocrine et paracrine, et indiquent que cela représente probablement un aspect important de la manière dont les neutrophiles façonnent leur environnement et leurs interactions cellulaires."],"dc:identifier.uri":["http://hdl.handle.net/11143/20599"],"dc:language.iso":["en"],"dc:publisher":["Université de Sherbrooke"],"dc:rights.uri":["http://creativecommons.org/licenses/by-nc-nd/2.5/ca/"],"dc:subject":["Neutrophils","Extracellular vesicles","Apoptosis","Chemokines","NETs","Autocrine","Vésicles extracellulaires"],"dc:title":["Human neutrophils generate extracellular vesicles that modulate their functional responses"],"dc:type":["Mémoire de maîtrise"],"thesis:degree_discipline":["Immunologie"],"thesis:degree_level":["Maîtrise"],"thesis:degree_name":["M. Sc."],"thesis:institution_name":["Faculté de médecine et des sciences de la santé"]},"updated_at":"2026-07-27T21:07:47Z"}