On the interdependence of pancreatic size, and tissue composition and cellularity, and growth : characterization of a caerulein-generated model of enhanced pancreatic growth in the adult male rat
type: CreativeWork
description: The aim of this investigation was to characterize the mutual relevance of changes in pancreatic organ-level indeces of cellular size, and of differentiated functional capacity, in relation to an index of cell proliferation within the pancreatic acinar parenchyma. We have characterized pancreatic wet weight, two major cellular biochemical constituents with respect to their glandular levels and in vivo biosynthesis levels (RNA, protein), tissue levels of two hydrolases (amylase and chymotrypsinogen), together with organ DNA levels (reflective of cellularity) in the normoplastic eutrophie gland of adult male Sprague-Dawley rats (a) during the course of a normal 24-hour activity-rest cycle under standard laboratory conditions, (b) in a caerulein model of secretagogue-enhanced whole organ functional activity and trophism (Solomon et al., 1983), and (c) under conditions of depressed functional activity through the implementation of in vivo cycloheximide regimes. Characterization of the time-structure of the pancreas in terms of its size, tissue composition, digestive potential (tissue levels of two hydrolases) and cellularity describe this organ as a highly heterogeneous entity within the circadian-time domain. Circadian-stage dependent profiles of changes in measurments for each parameter present parameter-specific characteristics). Biochemical parameters reflective of cell number and cell size present maxima during the diurnal phase of the cycle, while the converse is true for tissue hydrolase levels. RNA and protein biosynthetic activity present both daytime and nighttime peak values. The phasing of changes in parameters reflective of tissue fonction (amylase and chymotrypsin activity levels, protein and RNA biosynthesis), and tissue size, and cellularity suggest (a) the occurrence of a growth /restoration phase during the resting (diurnal) phase of the activity cycle in the gland, classically considered as a kinetically stable entity, and (b) a reverse phasing between…
abstract: The aim of this investigation was to characterize the mutual relevance of changes in pancreatic organ-level indeces of cellular size, and of differentiated functional capacity, in relation to an index of cell proliferation within the pancreatic acinar parenchyma. We have characterized pancreatic wet weight, two major cellular biochemical constituents with respect to their glandular levels and in vivo biosynthesis levels (RNA, protein), tissue levels of two hydrolases (amylase and chymotrypsinogen), together with organ DNA levels (reflective of cellularity) in the normoplastic eutrophie gland of adult male Sprague-Dawley rats (a) during the course of a normal 24-hour activity-rest cycle under standard laboratory conditions, (b) in a caerulein model of secretagogue-enhanced whole organ functional activity and trophism (Solomon et al., 1983), and (c) under conditions of depressed functional activity through the implementation of in vivo cycloheximide regimes. Characterization of the time-structure of the pancreas in terms of its size, tissue composition, digestive potential (tissue levels of two hydrolases) and cellularity describe this organ as a highly heterogeneous entity within the circadian-time domain. Circadian-stage dependent profiles of changes in measurments for each parameter present parameter-specific characteristics). Biochemical parameters reflective of cell number and cell size present maxima during the diurnal phase of the cycle, while the converse is true for tissue hydrolase levels. RNA and protein biosynthetic activity present both daytime and nighttime peak values. The phasing of changes in parameters reflective of tissue fonction (amylase and chymotrypsin activity levels, protein and RNA biosynthesis), and tissue size, and cellularity suggest (a) the occurrence of a growth /restoration phase during the resting (diurnal) phase of the activity cycle in the gland, classically considered as a kinetically stable entity, and (b) a reverse phasing between…
author: Genik, Paula Constance
language: en
keywords: Pancréas--Physiologie
date published: 1989
date modified: 2026-07-27T21:07:28Z
provider: Université de Sherbrooke
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Université de Sherbrooke

PropertyValue
property ID: Handle
value: http://hdl.handle.net/11143/15934