Effect of pomegranate extracts on apoptosis and cell cycle in haematological malignancies.
type: CreativeWork
description: Leukaemia is a complex form of blood malignancy characterized by the uncontrolled proliferation of haematopoietic cells and progressive accumulation of these cells within the bone marrow (BM) and secondary lymphoid tissues. The exact cause of leukaemia remains unknown. Leukaemia is a major problem worldwide affecting many people each year. However, current treatment options still have several limitations not least, the cytotoxicity of these therapies to normal cells and the fact that certain chemotherapy agents may cause bone marrow toxicity and organ damage. Several epidemiological studies have shown that high intake of fruit and vegetables are associated with low incidence of a number of human cancers. Researchers suggest that pomegranates contain bioactive chemicals with potential for treatment and prevention of cancer. Pomegranate juice (PJ) have been shown to inhibit cellular proliferation and tumor growth and induce cell death via apoptosis in a number of cancer cell lines. However, to date, few studies have investigated the potential of PJ in the treatment of Leukaemia. Here, PJ significantly induced apoptosis in 8 leukaemia cell lines and non tumour control cells although the lymphoid and 2 myeloid cell lines were affected to a greater extent than non-tumour control cells and 2 of the myeloid cell lines. Furthermore, PJ induced cell cycle arrest. These results provide evidence that PJ contain bioactive compounds that could be used in the treatment of Leukaemia.Treatment of four Leukaemia cell lines with five fractions obtained from PJ by solid phase extraction demonstrated that only the acetonitrile fractions decreased adenosine triphosphate (ATP) levels in all Leukaemia cell lines. Acetonitrile fractions also significantly activated caspase-3 and induced nuclear morphology characteristic of apoptosis. S phase arrest was induced by acetonitrile fractions which matched S phase arrest seen previously following whole PJ treatments. The acetonitrile fractions co…
abstract: Leukaemia is a complex form of blood malignancy characterized by the uncontrolled proliferation of haematopoietic cells and progressive accumulation of these cells within the bone marrow (BM) and secondary lymphoid tissues. The exact cause of leukaemia remains unknown. Leukaemia is a major problem worldwide affecting many people each year. However, current treatment options still have several limitations not least, the cytotoxicity of these therapies to normal cells and the fact that certain chemotherapy agents may cause bone marrow toxicity and organ damage. Several epidemiological studies have shown that high intake of fruit and vegetables are associated with low incidence of a number of human cancers. Researchers suggest that pomegranates contain bioactive chemicals with potential for treatment and prevention of cancer. Pomegranate juice (PJ) have been shown to inhibit cellular proliferation and tumor growth and induce cell death via apoptosis in a number of cancer cell lines. However, to date, few studies have investigated the potential of PJ in the treatment of Leukaemia. Here, PJ significantly induced apoptosis in 8 leukaemia cell lines and non tumour control cells although the lymphoid and 2 myeloid cell lines were affected to a greater extent than non-tumour control cells and 2 of the myeloid cell lines. Furthermore, PJ induced cell cycle arrest. These results provide evidence that PJ contain bioactive compounds that could be used in the treatment of Leukaemia.Treatment of four Leukaemia cell lines with five fractions obtained from PJ by solid phase extraction demonstrated that only the acetonitrile fractions decreased adenosine triphosphate (ATP) levels in all Leukaemia cell lines. Acetonitrile fractions also significantly activated caspase-3 and induced nuclear morphology characteristic of apoptosis. S phase arrest was induced by acetonitrile fractions which matched S phase arrest seen previously following whole PJ treatments. The acetonitrile fractions co…
author: Dahlawi, Haytham.
language: en
date published: 2013
date modified: 2026-07-24T06:31:18Z
provider: Sheffield Hallam University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Sheffield Hallam University (United Kingdom).