{"id":{"repo_id":"sheffield-hallam","oai_identifier":"oai:shura.shu.ac.uk:19454"},"canonical_url":"https://search.dev.ndltd.org/etd/sheffield-hallam/oai:shura.shu.ac.uk:19454","repository":{"repo_id":"sheffield-hallam","name":"Sheffield Hallam University","base_url":"https://shura.shu.ac.uk/cgi/oai2"},"display":{"title":"Phosphonium-functionalised gold nanoparticles for mitochondria targeted therapeutics.","abstract":"The work presented in this thesis demonstrates that triarylphosphoniopropyl-thiosulfate zwitterions and w-thioacetylpropyl(triphenyl)phosphonium salts can be used to prepare cationic, water-soluble gold nanoparticles with mean core sizes in the range of 2.5-5 nm. Phosphonium-functionalised gold nanoparticles have been characterised by a number of techniques including NMR, LDI-MS, SIMS, XPS, TGA, ICP-MS, MALDI-MS and TEM.Cytotoxicity studies illustrated that phosphonium ligands are relatively non-toxic to human prostate cancer cells and therefore can be used as a delivery vector to delivery gold nanoparticles specifically to the site of the mitochondria for other therapeutic applications such as photothermal therapy. Cellular uptake studies of phosphonium ligands by MALDI-MS showed that they are rapidly taken-up by cells within ten minutes.Phosphonium-functionalised gold nanoparticles are soluble in biological media which is of great importance for cell biology studies. Initial photothermal therapy studies demonstrated that the gold nanoparticles responds specifically to a green light excitation source (510-550 nm) which overlaps the surface plasmon resonance band of the phosphonium-functionalised gold nanoparticles at 525 nm. Preliminary data also showed that phosphonium-functionalised gold nanoparticles can selectively induce apoptosis in cells followed by irradiation, this was confirmed by Hoechst and caspase-3 staining. Quantification studies of phosphonium-functionalised gold nanoparticles by ICP-MS illustrated that these nanoparticle have good uptake in cells (above 75%). TEM data confirmed that phosphonium-functionalised gold nanoparticles are taken-up by human prostate cancer cells and are localised in the mitochondria.","abstract_html":"The work presented in this thesis demonstrates that triarylphosphoniopropyl-thiosulfate zwitterions and w-thioacetylpropyl(triphenyl)phosphonium salts can be used to prepare cationic, water-soluble gold nanoparticles with mean core sizes in the range of 2.5-5 nm. Phosphonium-functionalised gold nanoparticles have been characterised by a number of techniques including NMR, LDI-MS, SIMS, XPS, TGA, ICP-MS, MALDI-MS and TEM.Cytotoxicity studies illustrated that phosphonium ligands are relatively non-toxic to human prostate cancer cells and therefore can be used as a delivery vector to delivery gold nanoparticles specifically to the site of the mitochondria for other therapeutic applications such as photothermal therapy. Cellular uptake studies of phosphonium ligands by MALDI-MS showed that they are rapidly taken-up by cells within ten minutes.Phosphonium-functionalised gold nanoparticles are soluble in biological media which is of great importance for cell biology studies. Initial photothermal therapy studies demonstrated that the gold nanoparticles responds specifically to a green light excitation source (510-550 nm) which overlaps the surface plasmon resonance band of the phosphonium-functionalised gold nanoparticles at 525 nm. Preliminary data also showed that phosphonium-functionalised gold nanoparticles can selectively induce apoptosis in cells followed by irradiation, this was confirmed by Hoechst and caspase-3 staining. Quantification studies of phosphonium-functionalised gold nanoparticles by ICP-MS illustrated that these nanoparticle have good uptake in cells (above 75%). TEM data confirmed that phosphonium-functionalised gold nanoparticles are taken-up by human prostate cancer cells and are localised in the mitochondria.","abstract_has_math":false,"creators":["Chen, Yu-Su."],"institution":"Sheffield Hallam University (United Kingdom).","degree_name":"phd","degree_level":"doctoral","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Bricklebank, Neil","Cross, Neil"],"committee_chairs":[],"committee_members":[],"year":2014,"date_issued":"2014","date_published":"2014","updated_at":"2026-07-24T06:31:18Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Bricklebank, Neil","Cross, Neil"]},{"key":"dc:creator","label":"Author","values":["Chen, Yu-Su."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2014"]},{"key":"dc:date.issued","label":"Date","values":["2014"]},{"key":"dc:publisher.commercial","label":"Dc Publisher Commercial","values":["Sheffield Hallam University,"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Faculty of Arts Computing Engineering and Sciences."]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["Sheffield Hallam University (United Kingdom)."]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://shura.shu.ac.uk/19454/"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["doctoral"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["phd"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://shura.shu.ac.uk/19454/1/10694335.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The work presented in this thesis demonstrates that triarylphosphoniopropyl-thiosulfate zwitterions and w-thioacetylpropyl(triphenyl)phosphonium salts can be used to prepare cationic, water-soluble gold nanoparticles with mean core sizes in the range of 2.5-5 nm. Phosphonium-functionalised gold nanoparticles have been characterised by a number of techniques including NMR, LDI-MS, SIMS, XPS, TGA, ICP-MS, MALDI-MS and TEM.Cytotoxicity studies illustrated that phosphonium ligands are relatively non-toxic to human prostate cancer cells and therefore can be used as a delivery vector to delivery gold nanoparticles specifically to the site of the mitochondria for other therapeutic applications such as photothermal therapy. Cellular uptake studies of phosphonium ligands by MALDI-MS showed that they are rapidly taken-up by cells within ten minutes.Phosphonium-functionalised gold nanoparticles are soluble in biological media which is of great importance for cell biology studies. Initial photothermal therapy studies demonstrated that the gold nanoparticles responds specifically to a green light excitation source (510-550 nm) which overlaps the surface plasmon resonance band of the phosphonium-functionalised gold nanoparticles at 525 nm. Preliminary data also showed that phosphonium-functionalised gold nanoparticles can selectively induce apoptosis in cells followed by irradiation, this was confirmed by Hoechst and caspase-3 staining. Quantification studies of phosphonium-functionalised gold nanoparticles by ICP-MS illustrated that these nanoparticle have good uptake in cells (above 75%). TEM data confirmed that phosphonium-functionalised gold nanoparticles are taken-up by human prostate cancer cells and are localised in the mitochondria."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Phosphonium-functionalised gold nanoparticles for mitochondria targeted therapeutics."]}]}],"canonical_facts":{"dc:contributor.advisor":["Bricklebank, Neil","Cross, Neil"],"dc:creator":["Chen, Yu-Su."],"dc:date":["2014"],"dc:date.issued":["2014"],"dc:description.abstract":["The work presented in this thesis demonstrates that triarylphosphoniopropyl-thiosulfate zwitterions and w-thioacetylpropyl(triphenyl)phosphonium salts can be used to prepare cationic, water-soluble gold nanoparticles with mean core sizes in the range of 2.5-5 nm. Phosphonium-functionalised gold nanoparticles have been characterised by a number of techniques including NMR, LDI-MS, SIMS, XPS, TGA, ICP-MS, MALDI-MS and TEM.Cytotoxicity studies illustrated that phosphonium ligands are relatively non-toxic to human prostate cancer cells and therefore can be used as a delivery vector to delivery gold nanoparticles specifically to the site of the mitochondria for other therapeutic applications such as photothermal therapy. Cellular uptake studies of phosphonium ligands by MALDI-MS showed that they are rapidly taken-up by cells within ten minutes.Phosphonium-functionalised gold nanoparticles are soluble in biological media which is of great importance for cell biology studies. Initial photothermal therapy studies demonstrated that the gold nanoparticles responds specifically to a green light excitation source (510-550 nm) which overlaps the surface plasmon resonance band of the phosphonium-functionalised gold nanoparticles at 525 nm. Preliminary data also showed that phosphonium-functionalised gold nanoparticles can selectively induce apoptosis in cells followed by irradiation, this was confirmed by Hoechst and caspase-3 staining. Quantification studies of phosphonium-functionalised gold nanoparticles by ICP-MS illustrated that these nanoparticle have good uptake in cells (above 75%). TEM data confirmed that phosphonium-functionalised gold nanoparticles are taken-up by human prostate cancer cells and are localised in the mitochondria."],"dc:format":["application/pdf"],"dc:identifier.uri":["https://shura.shu.ac.uk/19454/1/10694335.pdf"],"dc:language":["en"],"dc:publisher.commercial":["Sheffield Hallam University,"],"dc:publisher.department":["Faculty of Arts Computing Engineering and Sciences."],"dc:publisher.institution":["Sheffield Hallam University (United Kingdom)."],"dc:relation.isreferencedby":["https://shura.shu.ac.uk/19454/"],"dc:title":["Phosphonium-functionalised gold nanoparticles for mitochondria targeted therapeutics."],"dc:type":["Thesis"],"dc:type.qualificationlevel":["doctoral"],"dc:type.qualificationname":["phd"]},"updated_at":"2026-07-24T06:31:18Z"}