Bacteriophages (phages) are viral obligate intracellular parasites that infect bacteria. Research has been carried out on alternative treatment plans for bacterial infections due to the increase in antibiotic resistance. Phages have been proposed as an alternative to antibiotics. This research focused on the isolation and characterization of bacteria from small aquariums. A total of 42 bacterial isolates were isolated from seven different fish tank water samples and were tested to determine if they possessed phages. Spot plaque assay revealed presence of lytic phage for only one of the isolated bacterial strains, which was determined to belong to Enterobacteriaceae family based on the 16S ribosomal RNA sequencing. The phage isolated in spot plaque assay was successfully propagated in liquid bacterial culture. TEM showed that the isolated phage had a complex capsid and according to its morphology probably belongs to the family Myoviridae. Analysis of phage’s genetic material indicated that it is a DNA phage with its highest DNA sequence similarity with previously described phages of bacteria from Enterobacteriaceae. Further analysis is required to determine if this phage belongs to a previously described phage species or not and if it has potential in the treatment of enterobacterial infections.
","abstract_html":"<p>Bacteriophages (phages) are viral obligate intracellular parasites that infect bacteria. Research has been carried out on alternative treatment plans for bacterial infections due to the increase in antibiotic resistance. Phages have been proposed as an alternative to antibiotics. This research focused on the isolation and characterization of bacteria from small aquariums. A total of 42 bacterial isolates were isolated from seven different fish tank water samples and were tested to determine if they possessed phages. Spot plaque assay revealed presence of lytic phage for only one of the isolated bacterial strains, which was determined to belong to <em>Enterobacteriaceae </em>family based on the 16S ribosomal RNA sequencing. The phage isolated in spot plaque assay was successfully propagated in liquid bacterial culture. TEM showed that the isolated phage had a complex capsid and according to its morphology probably belongs to the family <em>Myoviridae. </em>Analysis of phage’s genetic material indicated that it is a DNA phage with its highest DNA sequence similarity with previously described phages of bacteria from <em>Enterobacteriaceae</em>. Further analysis is required to determine if this phage belongs to a previously described phage species or not and if it has potential in the treatment of enterobacterial infections.</p>","abstract_has_math":false,"creators":["Oladele-Ajose, Oluwaseyi Oluwagbeminiyi"],"institution":null,"degree_name":"Master of Science - Biology","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Dr. Dan Bennett","Dr. Zeljko Radulovic","Dr. Josephine Taylor"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-12-16T08:00:00Z","date_published":"2023-12-16T08:00:00Z","updated_at":"2026-07-24T04:30:45Z","subjects":["Bacteriophages","viral obligate","Spot plaque assay","Myoviridae","complex capsid","Biology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarworks.sfasu.edu/etds/526","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dr. Dan Bennett","Dr. Zeljko Radulovic","Dr. Josephine Taylor"]},{"key":"dc:creator","label":"Author","values":["Oladele-Ajose, Oluwaseyi Oluwagbeminiyi"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2023-12-15T08:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science - Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Bacteriophages","viral obligate","Spot plaque assay","Myoviridae","complex capsid","Biology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarworks.sfasu.edu/etds/526"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Bacteriophages (phages) are viral obligate intracellular parasites that infect bacteria. Research has been carried out on alternative treatment plans for bacterial infections due to the increase in antibiotic resistance. Phages have been proposed as an alternative to antibiotics. This research focused on the isolation and characterization of bacteria from small aquariums. A total of 42 bacterial isolates were isolated from seven different fish tank water samples and were tested to determine if they possessed phages. Spot plaque assay revealed presence of lytic phage for only one of the isolated bacterial strains, which was determined to belong to Enterobacteriaceae family based on the 16S ribosomal RNA sequencing. The phage isolated in spot plaque assay was successfully propagated in liquid bacterial culture. TEM showed that the isolated phage had a complex capsid and according to its morphology probably belongs to the family Myoviridae. Analysis of phage’s genetic material indicated that it is a DNA phage with its highest DNA sequence similarity with previously described phages of bacteria from Enterobacteriaceae. Further analysis is required to determine if this phage belongs to a previously described phage species or not and if it has potential in the treatment of enterobacterial infections.
"]},{"key":"dc:title","label":"Title","values":["Isolation and Characterization of Bacteriophages from Fish tank Samples"]}]}],"canonical_facts":{"dc:contributor":["Dr. Dan Bennett","Dr. Zeljko Radulovic","Dr. Josephine Taylor"],"dc:creator":["Oladele-Ajose, Oluwaseyi Oluwagbeminiyi"],"dc:date.available":["2023-12-15T08:00:00Z"],"dc:description.abstract":["Bacteriophages (phages) are viral obligate intracellular parasites that infect bacteria. Research has been carried out on alternative treatment plans for bacterial infections due to the increase in antibiotic resistance. Phages have been proposed as an alternative to antibiotics. This research focused on the isolation and characterization of bacteria from small aquariums. A total of 42 bacterial isolates were isolated from seven different fish tank water samples and were tested to determine if they possessed phages. Spot plaque assay revealed presence of lytic phage for only one of the isolated bacterial strains, which was determined to belong to Enterobacteriaceae family based on the 16S ribosomal RNA sequencing. The phage isolated in spot plaque assay was successfully propagated in liquid bacterial culture. TEM showed that the isolated phage had a complex capsid and according to its morphology probably belongs to the family Myoviridae. Analysis of phage’s genetic material indicated that it is a DNA phage with its highest DNA sequence similarity with previously described phages of bacteria from Enterobacteriaceae. Further analysis is required to determine if this phage belongs to a previously described phage species or not and if it has potential in the treatment of enterobacterial infections.
"],"dc:identifier":["https://scholarworks.sfasu.edu/etds/526"],"dc:subject":["Bacteriophages","viral obligate","Spot plaque assay","Myoviridae","complex capsid","Biology"],"dc:title":["Isolation and Characterization of Bacteriophages from Fish tank Samples"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science - Biology"]},"updated_at":"2026-07-24T04:30:45Z"}