THE EFFECT OF HOLDING TIME OF WHOLE MARE BLOOD ON CONCENTRATION OF PROGESTERONE
The objective of this study was to evaluate if holding time had an effect on mare whole blood progesterone levels. This research was completed using 12 mares, with blood being collected on multiple cycles to get a total of 28 separate blood collections. At each collection 5 vials of blood were taken for different time treatments of 0, 12, 24, and 48 hours. The 0 hour was centrifuged within 30 mins of collection and the other 4 were refrigerated at 4℃ until the specific time treatment was finished. After they were centrifuged all the samples were stored in the freezer at -22℃ until they were transported to the lab. A total of 140 data points was analyzed for this research. On further analysis of the results, the conclusion is that there is no effect of time on concentration of progesterone in the whole blood of mares.
progesterone, mare, radioimmunoassay, degradation, pregnancy, Animal Sciences, Other Animal Sciences
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