{"id":{"repo_id":"sdstate","oai_identifier":"oai:openprairie.sdstate.edu:etd-2423"},"canonical_url":"https://search.dev.ndltd.org/etd/sdstate/oai:openprairie.sdstate.edu:etd-2423","repository":{"repo_id":"sdstate","name":"South Dakota State University","base_url":"https://openprairie.sdstate.edu/do/oai/"},"display":{"title":"Development of a Subunit Vaccine Against Bovine Diarrhea Caused by K99 Enterotoxigenic Escherichia coli and Bovine Viral Diarrhea Virus","abstract":"<p>Bovine diarrhea is economically one of the most important problems in cattle industry. Enterotoxigenic Escherichia coli (ETEC), particularly strains expressing K99 (F5) fimbriae and heat-stable type I (STa) enterotoxin is the predominant bacterial cause of diarrhea in calves; whereas bovine viral diarrhea virus (BVDV) causes diarrhea and other illness to cattle at all ages. A vaccine that prevents against both K99 fimbrial ETEC and BVDV could benefit greatly to cattle producers worldwide. We hypothesized that a novel multivalent vaccine composed of K99, STa and BVDV E2 antigens could induce immunity for broad protection against bovine diarrhea. In this study we applied FanC, the major fimbrial subunit of K99, as a backbone to have a STa toxoid and the most immunogenic E2 epitopes embedded to construct ‘FanC-STa-E2’ fusion antigen, and examined this ‘FanC-STa-E2’ fusion antigen for immunity against ETEC and BVDV in a murine model for vaccine potential assessment. Adult female BALB/C mouse intraperitoneal (i.p.) immunized with 200 μg purified antigen with equal volume of adjuvant. Developed systemic and mucosal immune responses to K99, STa, and BVDV E2. Moreover, those antibodies showed abilities to block the E. coli K99 bacterial adhesion by K99-receptor positive INT-407 and IPEC-J1 cells, neutralized the STa enterotoxin, and also neutralized the BVDV infection. This suggests that this fusion antigen has the potential to be developed as a broadly protective vaccine against bovine diarrhea.</p>","abstract_html":"&lt;p&gt;Bovine diarrhea is economically one of the most important problems in cattle industry. Enterotoxigenic Escherichia coli (ETEC), particularly strains expressing K99 (F5) fimbriae and heat-stable type I (STa) enterotoxin is the predominant bacterial cause of diarrhea in calves; whereas bovine viral diarrhea virus (BVDV) causes diarrhea and other illness to cattle at all ages. A vaccine that prevents against both K99 fimbrial ETEC and BVDV could benefit greatly to cattle producers worldwide. We hypothesized that a novel multivalent vaccine composed of K99, STa and BVDV E2 antigens could induce immunity for broad protection against bovine diarrhea. In this study we applied FanC, the major fimbrial subunit of K99, as a backbone to have a STa toxoid and the most immunogenic E2 epitopes embedded to construct ‘FanC-STa-E2’ fusion antigen, and examined this ‘FanC-STa-E2’ fusion antigen for immunity against ETEC and BVDV in a murine model for vaccine potential assessment. Adult female BALB/C mouse intraperitoneal (i.p.) immunized with 200 μg purified antigen with equal volume of adjuvant. Developed systemic and mucosal immune responses to K99, STa, and BVDV E2. Moreover, those antibodies showed abilities to block the E. coli K99 bacterial adhesion by K99-receptor positive INT-407 and IPEC-J1 cells, neutralized the STa enterotoxin, and also neutralized the BVDV infection. This suggests that this fusion antigen has the potential to be developed as a broadly protective vaccine against bovine diarrhea.&lt;/p&gt;","abstract_has_math":false,"creators":["Hashish, Emad"],"institution":null,"degree_name":"Doctor of Philosophy (PhD)","degree_level":"Dissertation - Open Access","degree_discipline":"Veterinary and Biomedical Sciences","degree_department":null,"school":null,"contributors":["Weiping Zhang"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-01-01T08:00:00Z","date_published":"2013-01-01T08:00:00Z","updated_at":"2026-07-24T04:29:08Z","subjects":["Veterinary Infectious Diseases","Veterinary Pathology and Pathobiology"],"languages":["en"],"rights":["<p>In Copyright - Educational Use Permitted<br /><a href=\"http://rightsstatements.org/vocab/InC-EDU/1.0/\">http://rightsstatements.org/vocab/InC-EDU/1.0/</a></p>"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://openprairie.sdstate.edu/etd/1422","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Weiping Zhang"]},{"key":"dc:creator","label":"Author","values":["Hashish, Emad"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2017-08-03T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Veterinary and Biomedical Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation - Open Access"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (PhD)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Veterinary Infectious Diseases","Veterinary Pathology and Pathobiology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["<p>In Copyright - Educational Use Permitted<br /><a href=\"http://rightsstatements.org/vocab/InC-EDU/1.0/\">http://rightsstatements.org/vocab/InC-EDU/1.0/</a></p>"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://openprairie.sdstate.edu/etd/1422"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["<p>Bovine diarrhea is economically one of the most important problems in cattle industry. Enterotoxigenic Escherichia coli (ETEC), particularly strains expressing K99 (F5) fimbriae and heat-stable type I (STa) enterotoxin is the predominant bacterial cause of diarrhea in calves; whereas bovine viral diarrhea virus (BVDV) causes diarrhea and other illness to cattle at all ages. A vaccine that prevents against both K99 fimbrial ETEC and BVDV could benefit greatly to cattle producers worldwide. We hypothesized that a novel multivalent vaccine composed of K99, STa and BVDV E2 antigens could induce immunity for broad protection against bovine diarrhea. In this study we applied FanC, the major fimbrial subunit of K99, as a backbone to have a STa toxoid and the most immunogenic E2 epitopes embedded to construct ‘FanC-STa-E2’ fusion antigen, and examined this ‘FanC-STa-E2’ fusion antigen for immunity against ETEC and BVDV in a murine model for vaccine potential assessment. Adult female BALB/C mouse intraperitoneal (i.p.) immunized with 200 μg purified antigen with equal volume of adjuvant. Developed systemic and mucosal immune responses to K99, STa, and BVDV E2. Moreover, those antibodies showed abilities to block the E. coli K99 bacterial adhesion by K99-receptor positive INT-407 and IPEC-J1 cells, neutralized the STa enterotoxin, and also neutralized the BVDV infection. This suggests that this fusion antigen has the potential to be developed as a broadly protective vaccine against bovine diarrhea.</p>"]},{"key":"dc:title","label":"Title","values":["Development of a Subunit Vaccine Against Bovine Diarrhea Caused by K99 Enterotoxigenic Escherichia coli and Bovine Viral Diarrhea Virus"]}]}],"canonical_facts":{"dc:contributor":["Weiping Zhang"],"dc:creator":["Hashish, Emad"],"dc:date.available":["2017-08-03T07:00:00Z"],"dc:description.abstract":["<p>Bovine diarrhea is economically one of the most important problems in cattle industry. Enterotoxigenic Escherichia coli (ETEC), particularly strains expressing K99 (F5) fimbriae and heat-stable type I (STa) enterotoxin is the predominant bacterial cause of diarrhea in calves; whereas bovine viral diarrhea virus (BVDV) causes diarrhea and other illness to cattle at all ages. A vaccine that prevents against both K99 fimbrial ETEC and BVDV could benefit greatly to cattle producers worldwide. We hypothesized that a novel multivalent vaccine composed of K99, STa and BVDV E2 antigens could induce immunity for broad protection against bovine diarrhea. In this study we applied FanC, the major fimbrial subunit of K99, as a backbone to have a STa toxoid and the most immunogenic E2 epitopes embedded to construct ‘FanC-STa-E2’ fusion antigen, and examined this ‘FanC-STa-E2’ fusion antigen for immunity against ETEC and BVDV in a murine model for vaccine potential assessment. Adult female BALB/C mouse intraperitoneal (i.p.) immunized with 200 μg purified antigen with equal volume of adjuvant. Developed systemic and mucosal immune responses to K99, STa, and BVDV E2. Moreover, those antibodies showed abilities to block the E. coli K99 bacterial adhesion by K99-receptor positive INT-407 and IPEC-J1 cells, neutralized the STa enterotoxin, and also neutralized the BVDV infection. This suggests that this fusion antigen has the potential to be developed as a broadly protective vaccine against bovine diarrhea.</p>"],"dc:identifier":["https://openprairie.sdstate.edu/etd/1422"],"dc:language":["en"],"dc:rights":["<p>In Copyright - Educational Use Permitted<br /><a href=\"http://rightsstatements.org/vocab/InC-EDU/1.0/\">http://rightsstatements.org/vocab/InC-EDU/1.0/</a></p>"],"dc:subject":["Veterinary Infectious Diseases","Veterinary Pathology and Pathobiology"],"dc:title":["Development of a Subunit Vaccine Against Bovine Diarrhea Caused by K99 Enterotoxigenic Escherichia coli and Bovine Viral Diarrhea Virus"],"thesis:degree_discipline":["Veterinary and Biomedical Sciences"],"thesis:degree_level":["Dissertation - Open Access"],"thesis:degree_name":["Doctor of Philosophy (PhD)"]},"updated_at":"2026-07-24T04:29:08Z"}