Characterization of Lung Inflammation Induced by Exposure to Fipronil
type: CreativeWork
description: Fipronil is an insecticide that acts at the gamma-aminobutyric acid receptor and glutamate-gated chloride channels in the central nervous systems of target organisms. The use of fipronil is increasing across the globe. Presently, very little data exist on the potential impact of exposure to fipronil on the lungs. We studied the same by exposing mice to fipronil intranasally (N=8) or orally (N=7) for 7 days followed by collection of blood, broncho-alveolar lavage (BAL) fluid and lung tissues. Control mice were given corn oil (N=15). The oral and intranasal exposure routes were chosen because these are the most common routes of exposure for humans and animals. Hematoxylin-eosin stained lung sections showed normal histology in the control lungs compared to the thickened alveolar septa, disruption of the airways epithelium and damage to vascular endothelium in the intranasal and the oral groups. Lung sections stained for von Willebrand factor showed that mice exposed to fipronil either orally or intranasally had increased staining in the endothelium and septal capillaries. Compared to the control mice, TLR4 expression in lungs from animals treated orally with fipronil was reduced while animals exposed intranasally had increased TLR4 staining in the airway epithelium. Similarly, TLR9 stained lungs showed that orally treated animals had reduced TLR9 reaction in the airway epithelial cells but intranasally exposed animals had intense TLR9 staining in the alveolar septa and airway epithelium. The slides were also scored blindly to gain a quantitative understanding of the staining; there were a significantly higher number of TLR4 positive stained cells in the intranasal fipronil group (P=0.010) but no significant differences between treatments for TLR9 positive stained cells (P=0.226). The U937 cell line was employed to compliment the in vivo work. Cells were exposed to fipronil in DMSO at concentrations of 0.29 µm to 5.72 µm per 1 ml for various times from 3, 9 and 24 hours…
abstract: Fipronil is an insecticide that acts at the gamma-aminobutyric acid receptor and glutamate-gated chloride channels in the central nervous systems of target organisms. The use of fipronil is increasing across the globe. Presently, very little data exist on the potential impact of exposure to fipronil on the lungs. We studied the same by exposing mice to fipronil intranasally (N=8) or orally (N=7) for 7 days followed by collection of blood, broncho-alveolar lavage (BAL) fluid and lung tissues. Control mice were given corn oil (N=15). The oral and intranasal exposure routes were chosen because these are the most common routes of exposure for humans and animals. Hematoxylin-eosin stained lung sections showed normal histology in the control lungs compared to the thickened alveolar septa, disruption of the airways epithelium and damage to vascular endothelium in the intranasal and the oral groups. Lung sections stained for von Willebrand factor showed that mice exposed to fipronil either orally or intranasally had increased staining in the endothelium and septal capillaries. Compared to the control mice, TLR4 expression in lungs from animals treated orally with fipronil was reduced while animals exposed intranasally had increased TLR4 staining in the airway epithelium. Similarly, TLR9 stained lungs showed that orally treated animals had reduced TLR9 reaction in the airway epithelial cells but intranasally exposed animals had intense TLR9 staining in the alveolar septa and airway epithelium. The slides were also scored blindly to gain a quantitative understanding of the staining; there were a significantly higher number of TLR4 positive stained cells in the intranasal fipronil group (P=0.010) but no significant differences between treatments for TLR9 positive stained cells (P=0.226). The U937 cell line was employed to compliment the in vivo work. Cells were exposed to fipronil in DMSO at concentrations of 0.29 µm to 5.72 µm per 1 ml for various times from 3, 9 and 24 hours…
author: Merkowsky, Kaitlin
language: eng
keywords: Fipronil Lungs Inflammation U937
date published: 2015-05-13
date modified: 2026-07-24T04:27:11Z
provider: University of Saskatchewan
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Saskatchewan

PropertyValue
property ID: Handle
value: https://hdl.handle.net/10388/ETD-2015-04-2039