{"id":{"repo_id":"sask","oai_identifier":"oai:harvest.usask.ca:10388/17266"},"canonical_url":"https://search.dev.ndltd.org/etd/sask/oai:harvest.usask.ca:10388/17266","repository":{"repo_id":"sask","name":"University of Saskatchewan","base_url":"https://harvest.usask.ca/server/oai/request"},"display":{"title":"THE FEEDBACK BETWEEN CELL SHAPE AND CYTOSKELETAL DYNAMICS ON CELL DIVISION AND EXPANSION IN EPIDERMAL PAVEMENT CELLS OF ARABIDOPSIS","abstract":"The epidermal layer of Arabidopsis thaliana (L.) Heynh. leaves plays a critical role in tissue formation, particularly through the development of lobes in the anticlinal walls of pavement cells, though curvature is also noted in the periclinal walls. The epidermal pavement cells form unique lobed shapes over time, while stomata, composed of guard cells, are identical in size and shape. Both cell types emerge from the same precursor cells and are produced through highly regulated divisions based on the morphology of the parent cell. Specifically, asymmetric divisions within the epidermis initiate stomatal lineages, where all divisions, excluding the terminal division, are asymmetric. Mutant lines with altered gene expression were selected based on phenotypes exhibiting altered cell division, expansion, and generation of specialized cells were used to evaluate hypotheses regarding sidewall curvature, mother cell bisection, and cell-division frequency. The pre-prophase band (PPB) microtubule array predicts the future division sites. This study explored a potential feedback loop between cell division and shape in Arabidopsis pavement cells, where lobes in the anticlinal cell wall curve towards the cell center, forming narrow regions that are hotspots for new cell divisions. In these divisions, the other cell area and intercepted sidewall segments were often bisected asymmetrically. Persistent microtubule bands at these sites can develop into PPBs, driving new cell wall formation and enhancing existing sidewall curvature. My research aims to determine how these lobes influence division sites and contribute to my understanding of the cytoskeletal regulation of cell division and expansion within the epidermal layer. My thesis focused on the development of the epidermal layer in Arabidopsis thaliana, emphasizing division orientation and microtubule activity in the epidermis.","abstract_html":"The epidermal layer of Arabidopsis thaliana (L.) Heynh. leaves plays a critical role in tissue formation, particularly through the development of lobes in the anticlinal walls of pavement cells, though curvature is also noted in the periclinal walls. The epidermal pavement cells form unique lobed shapes over time, while stomata, composed of guard cells, are identical in size and shape. Both cell types emerge from the same precursor cells and are produced through highly regulated divisions based on the morphology of the parent cell. Specifically, asymmetric divisions within the epidermis initiate stomatal lineages, where all divisions, excluding the terminal division, are asymmetric. Mutant lines with altered gene expression were selected based on phenotypes exhibiting altered cell division, expansion, and generation of specialized cells were used to evaluate hypotheses regarding sidewall curvature, mother cell bisection, and cell-division frequency. The pre-prophase band (PPB) microtubule array predicts the future division sites. This study explored a potential feedback loop between cell division and shape in Arabidopsis pavement cells, where lobes in the anticlinal cell wall curve towards the cell center, forming narrow regions that are hotspots for new cell divisions. In these divisions, the other cell area and intercepted sidewall segments were often bisected asymmetrically. Persistent microtubule bands at these sites can develop into PPBs, driving new cell wall formation and enhancing existing sidewall curvature. My research aims to determine how these lobes influence division sites and contribute to my understanding of the cytoskeletal regulation of cell division and expansion within the epidermal layer. My thesis focused on the development of the epidermal layer in Arabidopsis thaliana, emphasizing division orientation and microtubule activity in the epidermis.","abstract_has_math":false,"creators":["McEvoy, Delanie"],"institution":"University of Saskatchewan","degree_name":"Master of Science (M.Sc.)","degree_level":"Masters","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":[],"advisors":["Ambrose, Chris"],"committee_chairs":[],"committee_members":["Wei, Yangdou","Wilson, Ken","Wang, Hong","Webster, Nicole","Benson, James"],"year":2025,"date_issued":"2025-09-11","date_published":"2025-09-11","updated_at":"2026-07-24T04:26:49Z","subjects":["Arabidopsis","pavement cells","epidermis","microtubules","cell wall morphology"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/10388/17266","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Ambrose, Chris"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Wei, Yangdou","Wilson, Ken","Wang, Hong","Webster, Nicole","Benson, James"]},{"key":"dc:creator","label":"Author","values":["McEvoy, Delanie"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-09-11T21:26:41Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-09-11T21:26:41Z"]},{"key":"dc:date.issued","label":"Date","values":["2025-09-11"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (M.Sc.)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Saskatchewan"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Arabidopsis","pavement cells","epidermis","microtubules","cell wall morphology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10388/17266"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The epidermal layer of Arabidopsis thaliana (L.) Heynh. leaves plays a critical role in tissue formation, particularly through the development of lobes in the anticlinal walls of pavement cells, though curvature is also noted in the periclinal walls. The epidermal pavement cells form unique lobed shapes over time, while stomata, composed of guard cells, are identical in size and shape. Both cell types emerge from the same precursor cells and are produced through highly regulated divisions based on the morphology of the parent cell. Specifically, asymmetric divisions within the epidermis initiate stomatal lineages, where all divisions, excluding the terminal division, are asymmetric. Mutant lines with altered gene expression were selected based on phenotypes exhibiting altered cell division, expansion, and generation of specialized cells were used to evaluate hypotheses regarding sidewall curvature, mother cell bisection, and cell-division frequency. The pre-prophase band (PPB) microtubule array predicts the future division sites. This study explored a potential feedback loop between cell division and shape in Arabidopsis pavement cells, where lobes in the anticlinal cell wall curve towards the cell center, forming narrow regions that are hotspots for new cell divisions. In these divisions, the other cell area and intercepted sidewall segments were often bisected asymmetrically. Persistent microtubule bands at these sites can develop into PPBs, driving new cell wall formation and enhancing existing sidewall curvature. My research aims to determine how these lobes influence division sites and contribute to my understanding of the cytoskeletal regulation of cell division and expansion within the epidermal layer. My thesis focused on the development of the epidermal layer in Arabidopsis thaliana, emphasizing division orientation and microtubule activity in the epidermis."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["THE FEEDBACK BETWEEN CELL SHAPE AND CYTOSKELETAL DYNAMICS ON CELL DIVISION AND EXPANSION IN EPIDERMAL PAVEMENT CELLS OF ARABIDOPSIS"]}]}],"canonical_facts":{"dc:contributor.advisor":["Ambrose, Chris"],"dc:contributor.committeemember":["Wei, Yangdou","Wilson, Ken","Wang, Hong","Webster, Nicole","Benson, James"],"dc:creator":["McEvoy, Delanie"],"dc:date.accessioned":["2025-09-11T21:26:41Z"],"dc:date.available":["2025-09-11T21:26:41Z"],"dc:date.issued":["2025-09-11"],"dc:description.abstract":["The epidermal layer of Arabidopsis thaliana (L.) Heynh. leaves plays a critical role in tissue formation, particularly through the development of lobes in the anticlinal walls of pavement cells, though curvature is also noted in the periclinal walls. The epidermal pavement cells form unique lobed shapes over time, while stomata, composed of guard cells, are identical in size and shape. Both cell types emerge from the same precursor cells and are produced through highly regulated divisions based on the morphology of the parent cell. Specifically, asymmetric divisions within the epidermis initiate stomatal lineages, where all divisions, excluding the terminal division, are asymmetric. Mutant lines with altered gene expression were selected based on phenotypes exhibiting altered cell division, expansion, and generation of specialized cells were used to evaluate hypotheses regarding sidewall curvature, mother cell bisection, and cell-division frequency. The pre-prophase band (PPB) microtubule array predicts the future division sites. This study explored a potential feedback loop between cell division and shape in Arabidopsis pavement cells, where lobes in the anticlinal cell wall curve towards the cell center, forming narrow regions that are hotspots for new cell divisions. In these divisions, the other cell area and intercepted sidewall segments were often bisected asymmetrically. Persistent microtubule bands at these sites can develop into PPBs, driving new cell wall formation and enhancing existing sidewall curvature. My research aims to determine how these lobes influence division sites and contribute to my understanding of the cytoskeletal regulation of cell division and expansion within the epidermal layer. My thesis focused on the development of the epidermal layer in Arabidopsis thaliana, emphasizing division orientation and microtubule activity in the epidermis."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["https://hdl.handle.net/10388/17266"],"dc:language.iso":["en"],"dc:subject":["Arabidopsis","pavement cells","epidermis","microtubules","cell wall morphology"],"dc:title":["THE FEEDBACK BETWEEN CELL SHAPE AND CYTOSKELETAL DYNAMICS ON CELL DIVISION AND EXPANSION IN EPIDERMAL PAVEMENT CELLS OF ARABIDOPSIS"],"dc:type":["Thesis"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science (M.Sc.)"],"thesis:institution_name":["University of Saskatchewan"]},"updated_at":"2026-07-24T04:26:49Z"}