{"id":{"repo_id":"sask","oai_identifier":"oai:harvest.usask.ca:10388/15136"},"canonical_url":"https://search.dev.ndltd.org/etd/sask/oai:harvest.usask.ca:10388/15136","repository":{"repo_id":"sask","name":"University of Saskatchewan","base_url":"https://harvest.usask.ca/server/oai/request"},"display":{"title":"The creation of novel methodologies to examine the effects of phosphorus amendments on petroleum hydrocarbon bioremediation","abstract":"Successful soil petroleum hydrocarbon (PHC) bioremediation demands adequate phosphorus (P) concentrations. However, rapid adsorption and precipitation processes in calcareous soils lower labile soil P concentrations. As the P form impacts bioavailability, we hypothesized that the P amendment form would influence PHC degradation rates. However, soil characteristics, large Pi concentrations and inefficient P soil extractions limit the ability to reveal P amendment effects. Therefore, new methods, including analytical techniques and a generalized linear mixed model (GLMM), were created to investigate amendment fate and efficiency. The initial two studies focused on improving P analytical investigations. The first experiment created a stable isotope probing method using labelled (18O)-phosphate to monitor soil P dynamics. Soil received either unlabelled or labelled phosphate to monitor P transformations via sequential fractionation pools over six weeks. Since the unlabelled phosphate species is indistinguishable from endogenous soil P (94-144% of total soil P (Pt)), the mass balance of the 18O-labelled phosphate was more precise (67-85% Pt). As the role of subsoil organic P (Po) during PHC bioremediation is unknown, the second study aimed to improve P recovery from calcareous subsoils. After investigating pretreatments to a sodium hydroxide - ethylenediaminetetraacetic acid (EDTA) extraction, sodium acetate recovered the largest concentration of P from Po-doped calcareous subsoils (5.6% Pt) compared to other tested schemes (3.5-4.8% Pt). However, EDTA hindered MS Po quantification. While the analytical methodologies provide opportunities to speciate P and movement, it is not known whether the P bioavailability impacts PHC bioremediation. The final study investigated the influence of P amendments in low-activity (stalled) PHC-contaminated soils. A GLMM isolated the preferred environmental conditions for PHC removal in four sites. Predictions revealed benzene degradation increased as soil magnetic susceptibility and EC increased; however, this relationship is inversed for soil pH. Three fertilizer amendments (Na-phosphate, triethyl phosphate, and tripolyphosphate (TPP)) were delivered to low-activity soils to validate predictions. For one tested soil, TPP stimulated PHC removal; however, the other samples did not respond to any P amendments. The combination of novel analytical methodologies and a model-microcosm study provides new opportunities to elucidate the cycle of the macronutrient in PHC-contaminated calcareous subsoils.","abstract_html":"Successful soil petroleum hydrocarbon (PHC) bioremediation demands adequate phosphorus (P) concentrations. However, rapid adsorption and precipitation processes in calcareous soils lower labile soil P concentrations. As the P form impacts bioavailability, we hypothesized that the P amendment form would influence PHC degradation rates. However, soil characteristics, large Pi concentrations and inefficient P soil extractions limit the ability to reveal P amendment effects. Therefore, new methods, including analytical techniques and a generalized linear mixed model (GLMM), were created to investigate amendment fate and efficiency. The initial two studies focused on improving P analytical investigations. The first experiment created a stable isotope probing method using labelled (18O)-phosphate to monitor soil P dynamics. Soil received either unlabelled or labelled phosphate to monitor P transformations via sequential fractionation pools over six weeks. Since the unlabelled phosphate species is indistinguishable from endogenous soil P (94-144% of total soil P (Pt)), the mass balance of the 18O-labelled phosphate was more precise (67-85% Pt). As the role of subsoil organic P (Po) during PHC bioremediation is unknown, the second study aimed to improve P recovery from calcareous subsoils. After investigating pretreatments to a sodium hydroxide - ethylenediaminetetraacetic acid (EDTA) extraction, sodium acetate recovered the largest concentration of P from Po-doped calcareous subsoils (5.6% Pt) compared to other tested schemes (3.5-4.8% Pt). However, EDTA hindered MS Po quantification. While the analytical methodologies provide opportunities to speciate P and movement, it is not known whether the P bioavailability impacts PHC bioremediation. The final study investigated the influence of P amendments in low-activity (stalled) PHC-contaminated soils. A GLMM isolated the preferred environmental conditions for PHC removal in four sites. Predictions revealed benzene degradation increased as soil magnetic susceptibility and EC increased; however, this relationship is inversed for soil pH. Three fertilizer amendments (Na-phosphate, triethyl phosphate, and tripolyphosphate (TPP)) were delivered to low-activity soils to validate predictions. For one tested soil, TPP stimulated PHC removal; however, the other samples did not respond to any P amendments. The combination of novel analytical methodologies and a model-microcosm study provides new opportunities to elucidate the cycle of the macronutrient in PHC-contaminated calcareous subsoils.","abstract_has_math":false,"creators":["Schryer, Aimee D"],"institution":"University of Saskatchewan","degree_name":"Doctor of Philosophy (Ph.D.)","degree_level":"Doctoral","degree_discipline":"Soil Science","degree_department":null,"school":null,"contributors":[],"advisors":["Siciliano, Steven D"],"committee_chairs":[],"committee_members":["Peak, Derek","El-Aneed, Anas","Cade-Menun, Barbara","Schoenau, Jeff","Schneider, Kimberley"],"year":2023,"date_issued":"2023-10-12","date_published":"2023-10-12","updated_at":"2026-07-24T04:26:49Z","subjects":["phosphorus","petroleum hydrocarbons","bioremediation"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/10388/15136","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Siciliano, Steven D"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Peak, Derek","El-Aneed, Anas","Cade-Menun, Barbara","Schoenau, Jeff","Schneider, Kimberley"]},{"key":"dc:creator","label":"Author","values":["Schryer, Aimee D"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2023-10-12T22:19:00Z"]},{"key":"dc:date.issued","label":"Date","values":["2023-10-12"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Soil Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (Ph.D.)"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Saskatchewan"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["phosphorus","petroleum hydrocarbons","bioremediation"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/10388/15136"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Successful soil petroleum hydrocarbon (PHC) bioremediation demands adequate phosphorus (P) concentrations. However, rapid adsorption and precipitation processes in calcareous soils lower labile soil P concentrations. As the P form impacts bioavailability, we hypothesized that the P amendment form would influence PHC degradation rates. However, soil characteristics, large Pi concentrations and inefficient P soil extractions limit the ability to reveal P amendment effects. Therefore, new methods, including analytical techniques and a generalized linear mixed model (GLMM), were created to investigate amendment fate and efficiency. The initial two studies focused on improving P analytical investigations. The first experiment created a stable isotope probing method using labelled (18O)-phosphate to monitor soil P dynamics. Soil received either unlabelled or labelled phosphate to monitor P transformations via sequential fractionation pools over six weeks. Since the unlabelled phosphate species is indistinguishable from endogenous soil P (94-144% of total soil P (Pt)), the mass balance of the 18O-labelled phosphate was more precise (67-85% Pt). As the role of subsoil organic P (Po) during PHC bioremediation is unknown, the second study aimed to improve P recovery from calcareous subsoils. After investigating pretreatments to a sodium hydroxide - ethylenediaminetetraacetic acid (EDTA) extraction, sodium acetate recovered the largest concentration of P from Po-doped calcareous subsoils (5.6% Pt) compared to other tested schemes (3.5-4.8% Pt). However, EDTA hindered MS Po quantification. While the analytical methodologies provide opportunities to speciate P and movement, it is not known whether the P bioavailability impacts PHC bioremediation. The final study investigated the influence of P amendments in low-activity (stalled) PHC-contaminated soils. A GLMM isolated the preferred environmental conditions for PHC removal in four sites. Predictions revealed benzene degradation increased as soil magnetic susceptibility and EC increased; however, this relationship is inversed for soil pH. Three fertilizer amendments (Na-phosphate, triethyl phosphate, and tripolyphosphate (TPP)) were delivered to low-activity soils to validate predictions. For one tested soil, TPP stimulated PHC removal; however, the other samples did not respond to any P amendments. The combination of novel analytical methodologies and a model-microcosm study provides new opportunities to elucidate the cycle of the macronutrient in PHC-contaminated calcareous subsoils."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The creation of novel methodologies to examine the effects of phosphorus amendments on petroleum hydrocarbon bioremediation"]}]}],"canonical_facts":{"dc:contributor.advisor":["Siciliano, Steven D"],"dc:contributor.committeemember":["Peak, Derek","El-Aneed, Anas","Cade-Menun, Barbara","Schoenau, Jeff","Schneider, Kimberley"],"dc:creator":["Schryer, Aimee D"],"dc:date.accessioned":["2023-10-12T22:19:00Z"],"dc:date.issued":["2023-10-12"],"dc:description.abstract":["Successful soil petroleum hydrocarbon (PHC) bioremediation demands adequate phosphorus (P) concentrations. However, rapid adsorption and precipitation processes in calcareous soils lower labile soil P concentrations. As the P form impacts bioavailability, we hypothesized that the P amendment form would influence PHC degradation rates. However, soil characteristics, large Pi concentrations and inefficient P soil extractions limit the ability to reveal P amendment effects. Therefore, new methods, including analytical techniques and a generalized linear mixed model (GLMM), were created to investigate amendment fate and efficiency. The initial two studies focused on improving P analytical investigations. The first experiment created a stable isotope probing method using labelled (18O)-phosphate to monitor soil P dynamics. Soil received either unlabelled or labelled phosphate to monitor P transformations via sequential fractionation pools over six weeks. Since the unlabelled phosphate species is indistinguishable from endogenous soil P (94-144% of total soil P (Pt)), the mass balance of the 18O-labelled phosphate was more precise (67-85% Pt). As the role of subsoil organic P (Po) during PHC bioremediation is unknown, the second study aimed to improve P recovery from calcareous subsoils. After investigating pretreatments to a sodium hydroxide - ethylenediaminetetraacetic acid (EDTA) extraction, sodium acetate recovered the largest concentration of P from Po-doped calcareous subsoils (5.6% Pt) compared to other tested schemes (3.5-4.8% Pt). However, EDTA hindered MS Po quantification. While the analytical methodologies provide opportunities to speciate P and movement, it is not known whether the P bioavailability impacts PHC bioremediation. The final study investigated the influence of P amendments in low-activity (stalled) PHC-contaminated soils. A GLMM isolated the preferred environmental conditions for PHC removal in four sites. Predictions revealed benzene degradation increased as soil magnetic susceptibility and EC increased; however, this relationship is inversed for soil pH. Three fertilizer amendments (Na-phosphate, triethyl phosphate, and tripolyphosphate (TPP)) were delivered to low-activity soils to validate predictions. For one tested soil, TPP stimulated PHC removal; however, the other samples did not respond to any P amendments. The combination of novel analytical methodologies and a model-microcosm study provides new opportunities to elucidate the cycle of the macronutrient in PHC-contaminated calcareous subsoils."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["https://hdl.handle.net/10388/15136"],"dc:language.iso":["en"],"dc:subject":["phosphorus","petroleum hydrocarbons","bioremediation"],"dc:title":["The creation of novel methodologies to examine the effects of phosphorus amendments on petroleum hydrocarbon bioremediation"],"dc:type":["Thesis"],"thesis:degree_discipline":["Soil Science"],"thesis:degree_level":["Doctoral"],"thesis:degree_name":["Doctor of Philosophy (Ph.D.)"],"thesis:institution_name":["University of Saskatchewan"]},"updated_at":"2026-07-24T04:26:49Z"}