{"id":{"repo_id":"salford","oai_identifier":"oai:salford-repository.worktribe.com:1339184"},"canonical_url":"https://search.dev.ndltd.org/etd/salford/oai:salford-repository.worktribe.com:1339184","repository":{"repo_id":"salford","name":"U. of Salford","base_url":"https://salford-repository.worktribe.com/oaiprovider"},"display":{"title":"Studies on protozoan parasites of small mammals","abstract":"Studies on several different protozoan parasites of small mammals,moles (Talpa europaea), bank voles (Clethrionomys glareolus), fieldvoles (Microtus agrestis), field mice (Apodemus sylvaticus), mice,rats and rabbits are described in this study.In the mole (Talpa europaea) three parasites were studied:Elleipsisoma thornsoni, Cyclospora talpae and Trypanosoma talpae.Elleipsisoma thornsoni was described as a parasite in the red bloodcells of the mole earlier this century and its identity and parasiticnature was in doubt* Electron microscopic studies conclusively showedthat the parasite was an Apicomplexan and was different from otherblood parasites of haemosporidians and piroplasms by the presence of aconoid.Cyclospora talpae, a coccidian in the liver of the mole, wasstudied by both the light and electron microscope. In histologicalsections, the localisation of macro- and microgametocytes in theepithelial cells of the bile ducts was investigated. Theultrastructure of the sexual and asexual stages were studied.In Trypanosoma (Megatrypanum) talpae, some preliminary aspects ofits reproduction in the mammalian host and the development inhaemogamasid mites was studied. This is the first recorded observationof the development of mammalian trypanosomes in mites. In these mitesan isosporan-type coccidian (with developmental stages of sporogonyand gametogony) was also found and described. The sporulation ofoocysts was completed inside the mite's body and was typicallyisosporan with 2 sporocysts, each with 4 sporozoites.The second part of this study was concerned with the biology ofthe trypansomes of the subgenus Herpetosoma: T. microti. T. evotomysT. grosi, T. lewisi w/2, T. musculi P/2 and T. nabiasi. Cultivation ofT. microti, T. evotomys and T. grosi in insect tissue culture mediaand their ultrastructure is reported and compared with T. lewisi andT. musculi. T. nabiasi was grown in blood agar medium.T. microti and T. musculi were grown in Microtus agrestis embryofibroblasts to study development in vitro at 37°C of the bloodstreamand reproductive forms. T. microti, T. musculi P/2 and T. lewisi w/2were also grown for the first time in a new semi-defined, cell-freemedium to study development of the mammalian forms at 37°C.The successful cultivation of Herpetosoma trypanosomes made itpossible to conduct the biochemical studies, on thin-layer starch-gelelectrophoresis, of six trypanosome species (T. microti, T. evotomys,T. grosi, T. lewisiw/2, T. musculi P/2 and T. nabiasi) using13 isoenzymes. Results from 10 isoenzymes of these trypanosomes,except T. nabiasi which was available only as a weak lysate, wereanalysed by two computer programmes which constructed both asimilarity index and a cladistic relationship. The results were inaccord with the previous groupings of these trypanosomes based onobservations of methods of reproduction in mammals and development invectors.","abstract_html":"Studies on several different protozoan parasites of small mammals,moles (Talpa europaea), bank voles (Clethrionomys glareolus), fieldvoles (Microtus agrestis), field mice (Apodemus sylvaticus), mice,rats and rabbits are described in this study.In the mole (Talpa europaea) three parasites were studied:Elleipsisoma thornsoni, Cyclospora talpae and Trypanosoma talpae.Elleipsisoma thornsoni was described as a parasite in the red bloodcells of the mole earlier this century and its identity and parasiticnature was in doubt* Electron microscopic studies conclusively showedthat the parasite was an Apicomplexan and was different from otherblood parasites of haemosporidians and piroplasms by the presence of aconoid.Cyclospora talpae, a coccidian in the liver of the mole, wasstudied by both the light and electron microscope. In histologicalsections, the localisation of macro- and microgametocytes in theepithelial cells of the bile ducts was investigated. Theultrastructure of the sexual and asexual stages were studied.In Trypanosoma (Megatrypanum) talpae, some preliminary aspects ofits reproduction in the mammalian host and the development inhaemogamasid mites was studied. This is the first recorded observationof the development of mammalian trypanosomes in mites. In these mitesan isosporan-type coccidian (with developmental stages of sporogonyand gametogony) was also found and described. The sporulation ofoocysts was completed inside the mite's body and was typicallyisosporan with 2 sporocysts, each with 4 sporozoites.The second part of this study was concerned with the biology ofthe trypansomes of the subgenus Herpetosoma: T. microti. T. evotomysT. grosi, T. lewisi w/2, T. musculi P/2 and T. nabiasi. Cultivation ofT. microti, T. evotomys and T. grosi in insect tissue culture mediaand their ultrastructure is reported and compared with T. lewisi andT. musculi. T. nabiasi was grown in blood agar medium.T. microti and T. musculi were grown in Microtus agrestis embryofibroblasts to study development in vitro at 37°C of the bloodstreamand reproductive forms. T. microti, T. musculi P/2 and T. lewisi w/2were also grown for the first time in a new semi-defined, cell-freemedium to study development of the mammalian forms at 37°C.The successful cultivation of Herpetosoma trypanosomes made itpossible to conduct the biochemical studies, on thin-layer starch-gelelectrophoresis, of six trypanosome species (T. microti, T. evotomys,T. grosi, T. lewisiw/2, T. musculi P/2 and T. nabiasi) using13 isoenzymes. Results from 10 isoenzymes of these trypanosomes,except T. nabiasi which was available only as a weak lysate, wereanalysed by two computer programmes which constructed both asimilarity index and a cladistic relationship. The results were inaccord with the previous groupings of these trypanosomes based onobservations of methods of reproduction in mammals and development invectors.","abstract_has_math":false,"creators":["Mohamed, HA"],"institution":null,"degree_name":null,"degree_level":"Doctoral (Level 8)","degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1986,"date_issued":"1986","date_published":"1986","updated_at":"2026-07-24T04:26:14Z","subjects":[],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:salford-repository.worktribe.com:1339184"],"render_values":[{"text":"oai:salford-repository.worktribe.com:1339184","href":null,"code":true}]}]},"links":{"outbound_url":null,"outbound_label":null,"outbound_source":null},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.sponsor","label":"Sponsor","values":["British Council","#1 FUNDER NOT LISTED"]},{"key":"dc:creator","label":"Author","values":["Mohamed, HA"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["1986-01-01"]},{"key":"dc:date.issued","label":"Date","values":["1986"]},{"key":"dc:relation.isreferencedby","label":"Dc Relation Isreferencedby","values":["https://salford-repository.worktribe.com/output/1339184"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Doctoral (Level 8)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["oai:salford-repository.worktribe.com:1339184"]},{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://salford-repository.worktribe.com/file/1339184/1/5602149788.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Studies on several different protozoan parasites of small mammals,moles (Talpa europaea), bank voles (Clethrionomys glareolus), fieldvoles (Microtus agrestis), field mice (Apodemus sylvaticus), mice,rats and rabbits are described in this study.In the mole (Talpa europaea) three parasites were studied:Elleipsisoma thornsoni, Cyclospora talpae and Trypanosoma talpae.Elleipsisoma thornsoni was described as a parasite in the red bloodcells of the mole earlier this century and its identity and parasiticnature was in doubt* Electron microscopic studies conclusively showedthat the parasite was an Apicomplexan and was different from otherblood parasites of haemosporidians and piroplasms by the presence of aconoid.Cyclospora talpae, a coccidian in the liver of the mole, wasstudied by both the light and electron microscope. In histologicalsections, the localisation of macro- and microgametocytes in theepithelial cells of the bile ducts was investigated. Theultrastructure of the sexual and asexual stages were studied.In Trypanosoma (Megatrypanum) talpae, some preliminary aspects ofits reproduction in the mammalian host and the development inhaemogamasid mites was studied. This is the first recorded observationof the development of mammalian trypanosomes in mites. In these mitesan isosporan-type coccidian (with developmental stages of sporogonyand gametogony) was also found and described. The sporulation ofoocysts was completed inside the mite's body and was typicallyisosporan with 2 sporocysts, each with 4 sporozoites.The second part of this study was concerned with the biology ofthe trypansomes of the subgenus Herpetosoma: T. microti. T. evotomysT. grosi, T. lewisi w/2, T. musculi P/2 and T. nabiasi. Cultivation ofT. microti, T. evotomys and T. grosi in insect tissue culture mediaand their ultrastructure is reported and compared with T. lewisi andT. musculi. T. nabiasi was grown in blood agar medium.T. microti and T. musculi were grown in Microtus agrestis embryofibroblasts to study development in vitro at 37°C of the bloodstreamand reproductive forms. T. microti, T. musculi P/2 and T. lewisi w/2were also grown for the first time in a new semi-defined, cell-freemedium to study development of the mammalian forms at 37°C.The successful cultivation of Herpetosoma trypanosomes made itpossible to conduct the biochemical studies, on thin-layer starch-gelelectrophoresis, of six trypanosome species (T. microti, T. evotomys,T. grosi, T. lewisiw/2, T. musculi P/2 and T. nabiasi) using13 isoenzymes. Results from 10 isoenzymes of these trypanosomes,except T. nabiasi which was available only as a weak lysate, wereanalysed by two computer programmes which constructed both asimilarity index and a cladistic relationship. The results were inaccord with the previous groupings of these trypanosomes based onobservations of methods of reproduction in mammals and development invectors."]},{"key":"dc:title","label":"Title","values":["Studies on protozoan parasites of small mammals"]}]}],"canonical_facts":{"dc:contributor.sponsor":["British Council","#1 FUNDER NOT LISTED"],"dc:creator":["Mohamed, HA"],"dc:date":["1986-01-01"],"dc:date.issued":["1986"],"dc:description.abstract":["Studies on several different protozoan parasites of small mammals,moles (Talpa europaea), bank voles (Clethrionomys glareolus), fieldvoles (Microtus agrestis), field mice (Apodemus sylvaticus), mice,rats and rabbits are described in this study.In the mole (Talpa europaea) three parasites were studied:Elleipsisoma thornsoni, Cyclospora talpae and Trypanosoma talpae.Elleipsisoma thornsoni was described as a parasite in the red bloodcells of the mole earlier this century and its identity and parasiticnature was in doubt* Electron microscopic studies conclusively showedthat the parasite was an Apicomplexan and was different from otherblood parasites of haemosporidians and piroplasms by the presence of aconoid.Cyclospora talpae, a coccidian in the liver of the mole, wasstudied by both the light and electron microscope. In histologicalsections, the localisation of macro- and microgametocytes in theepithelial cells of the bile ducts was investigated. Theultrastructure of the sexual and asexual stages were studied.In Trypanosoma (Megatrypanum) talpae, some preliminary aspects ofits reproduction in the mammalian host and the development inhaemogamasid mites was studied. This is the first recorded observationof the development of mammalian trypanosomes in mites. In these mitesan isosporan-type coccidian (with developmental stages of sporogonyand gametogony) was also found and described. The sporulation ofoocysts was completed inside the mite's body and was typicallyisosporan with 2 sporocysts, each with 4 sporozoites.The second part of this study was concerned with the biology ofthe trypansomes of the subgenus Herpetosoma: T. microti. T. evotomysT. grosi, T. lewisi w/2, T. musculi P/2 and T. nabiasi. Cultivation ofT. microti, T. evotomys and T. grosi in insect tissue culture mediaand their ultrastructure is reported and compared with T. lewisi andT. musculi. T. nabiasi was grown in blood agar medium.T. microti and T. musculi were grown in Microtus agrestis embryofibroblasts to study development in vitro at 37°C of the bloodstreamand reproductive forms. T. microti, T. musculi P/2 and T. lewisi w/2were also grown for the first time in a new semi-defined, cell-freemedium to study development of the mammalian forms at 37°C.The successful cultivation of Herpetosoma trypanosomes made itpossible to conduct the biochemical studies, on thin-layer starch-gelelectrophoresis, of six trypanosome species (T. microti, T. evotomys,T. grosi, T. lewisiw/2, T. musculi P/2 and T. nabiasi) using13 isoenzymes. Results from 10 isoenzymes of these trypanosomes,except T. nabiasi which was available only as a weak lysate, wereanalysed by two computer programmes which constructed both asimilarity index and a cladistic relationship. The results were inaccord with the previous groupings of these trypanosomes based onobservations of methods of reproduction in mammals and development invectors."],"dc:identifier":["oai:salford-repository.worktribe.com:1339184"],"dc:identifier.uri":["https://salford-repository.worktribe.com/file/1339184/1/5602149788.pdf"],"dc:language":["en"],"dc:relation.isreferencedby":["https://salford-repository.worktribe.com/output/1339184"],"dc:title":["Studies on protozoan parasites of small mammals"],"dc:type":["Thesis"],"dc:type.qualificationlevel":["Doctoral (Level 8)"]},"updated_at":"2026-07-24T04:26:14Z"}