Characterisation of a panel of recombinant antigens from Echinococcus granulosus and their role in the immunological follow up, after treatment, of cystic hydatid patients from Turkana, Kenya
type: CreativeWork
description: Cystic echinococcosis is a serious zoonotic disease caused by the metacestodeof Echinoccoccus granulosus inflicting significant morbidity and mortality inmany parts of the world. Treatment of human hydatid cases involveschemotherapy with the drug Albendazole which has beneficial effects in somecases but not others. Disease progression can be monitored by ultrasoundusing the WHO classification system, but this only indicates large scalechanges in cyst morphology. Antibody responses to cyst fluid antigens havepreviously been used to provide further information on follow up after treatmentand the current project explores the usefulness of a panel of novel recombinantantigens in mapping antibody responses to cyst morphological changes.Positive clones pET19b-EgFABP1-C10, pET19b-EgHSP70-C10, pET19b-EgTPx-2Cb, pET19b-EgEF1-p/6-C9 and pET19b-EgAgB-C7 were selected andused to synthesise recombinant fatty acid binding protein 1 (EgFABPI), heatshock protein 70 (EgHSP70), thioredoxin peroxidase (EgfTPx) elongation factor1-beta/delta (EgEF1-(3/6) and antigen B (EgAgB) fusion proteins respectivelycarrying N-terminal His-tagged sequences. A panel of defined sequential seraof Turkana origin (Kenya), untreated (n =9) and treated (n =22) was probed inELISA with the recombinant parasite antigens. Results show that differentantigens react differently towards total IgG and lgG1 and lgG4 isotype subclassantibodies. Some antigens are associated with fluctuation in antibody levels ator during the time of transition from one cyst morphology to that of another type,whilst other antigens show little or no difference in reactivity. In some cases,fluctuating levels of antibody are correlated to pre and post treatment withalbendazole. Antibodies are rarely static and fluctuate up and down during andafter treatment, indicating a more varied release of extraneous antigenicmaterial supplementary to those antigens normally associated with hydatid cystfluid. In a number of cases particular antibody p…
abstract: Cystic echinococcosis is a serious zoonotic disease caused by the metacestodeof Echinoccoccus granulosus inflicting significant morbidity and mortality inmany parts of the world. Treatment of human hydatid cases involveschemotherapy with the drug Albendazole which has beneficial effects in somecases but not others. Disease progression can be monitored by ultrasoundusing the WHO classification system, but this only indicates large scalechanges in cyst morphology. Antibody responses to cyst fluid antigens havepreviously been used to provide further information on follow up after treatmentand the current project explores the usefulness of a panel of novel recombinantantigens in mapping antibody responses to cyst morphological changes.Positive clones pET19b-EgFABP1-C10, pET19b-EgHSP70-C10, pET19b-EgTPx-2Cb, pET19b-EgEF1-p/6-C9 and pET19b-EgAgB-C7 were selected andused to synthesise recombinant fatty acid binding protein 1 (EgFABPI), heatshock protein 70 (EgHSP70), thioredoxin peroxidase (EgfTPx) elongation factor1-beta/delta (EgEF1-(3/6) and antigen B (EgAgB) fusion proteins respectivelycarrying N-terminal His-tagged sequences. A panel of defined sequential seraof Turkana origin (Kenya), untreated (n =9) and treated (n =22) was probed inELISA with the recombinant parasite antigens. Results show that differentantigens react differently towards total IgG and lgG1 and lgG4 isotype subclassantibodies. Some antigens are associated with fluctuation in antibody levels ator during the time of transition from one cyst morphology to that of another type,whilst other antigens show little or no difference in reactivity. In some cases,fluctuating levels of antibody are correlated to pre and post treatment withalbendazole. Antibodies are rarely static and fluctuate up and down during andafter treatment, indicating a more varied release of extraneous antigenicmaterial supplementary to those antigens normally associated with hydatid cystfluid. In a number of cases particular antibody p…
author: Bodell, AJ
language: en
date published: 2012
date modified: 2026-07-24T04:26:12Z
provider: U. of Salford
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: U. of Salford