Investigating the use of omega three fish oils as protective agents against cytokine-induced skeletal muscle damage
type: CreativeWork
description: IntroductionMuscle injury and disease impacts on the lives of adults and children, contributing tosevere morbidity and increased mortality e.g. Muscular dystrophy affects over 70,000people UK wide (Muscular Dystrophy UK). Treatment of muscle disorders contributeto unemployment rates and cost the NHS billions of pounds per year. Growth factorsand cytokines regulate skeletal muscle homeostasis and growth and cytokinedysregulation may cause irreversible damage. Currently, poorly effective therapiesfor muscle injury include non-steroidal/ steroidal anti-inflammatory drugs andphysiotherapy.Previous research indicates omega three fish oils including Eicosapentaenoic acid(ERA), may offer protective anti-fibrotic properties and favoring useful repairprocesses. ERA decreases muscle protein degradation in cancer patients (Smith etal 2005) and increases the rate of protein synthesis in elderly patients (Smith et al2011). In vitro studies show ERA down-regulates chronic inflammation via inhibitionof NFKB activation (Huang et al 2011) and fibrosis via suppression of TransformingGrowth Factor-Pi (TGF-pi,Das 2004). Our group showed ERA protection fromcytokine (TNFa) induced muscular atrophy in a murine cell model (Magee et al2008). Further, ERA may have intracellular signaling component interactions and bedependent on PPARy (Magee et al 2012). ERA and DMA interact with lipid rafts (Liet al 2005) therefore may be able to displace receptors from signaling domains.Development of omega 3 fatty acid for therapeutic uses in fibrosis and tissue repairrequires a deeper understanding of additional intracellular signaling components,which is facilitated by a wider range of in vitro models.ObjectivesTo establish whether ERA, and its relative Docosahexaenoic acid (DMA), couldprevent cytokine-induced damage in models of atrophy and pathological fibrosis andidentify some of the mechanisms involved.MethodsUsing the cell line, C2C12, a well established model of myogenesis, ERA and DMAwere investi…
abstract: IntroductionMuscle injury and disease impacts on the lives of adults and children, contributing tosevere morbidity and increased mortality e.g. Muscular dystrophy affects over 70,000people UK wide (Muscular Dystrophy UK). Treatment of muscle disorders contributeto unemployment rates and cost the NHS billions of pounds per year. Growth factorsand cytokines regulate skeletal muscle homeostasis and growth and cytokinedysregulation may cause irreversible damage. Currently, poorly effective therapiesfor muscle injury include non-steroidal/ steroidal anti-inflammatory drugs andphysiotherapy.Previous research indicates omega three fish oils including Eicosapentaenoic acid(ERA), may offer protective anti-fibrotic properties and favoring useful repairprocesses. ERA decreases muscle protein degradation in cancer patients (Smith etal 2005) and increases the rate of protein synthesis in elderly patients (Smith et al2011). In vitro studies show ERA down-regulates chronic inflammation via inhibitionof NFKB activation (Huang et al 2011) and fibrosis via suppression of TransformingGrowth Factor-Pi (TGF-pi,Das 2004). Our group showed ERA protection fromcytokine (TNFa) induced muscular atrophy in a murine cell model (Magee et al2008). Further, ERA may have intracellular signaling component interactions and bedependent on PPARy (Magee et al 2012). ERA and DMA interact with lipid rafts (Liet al 2005) therefore may be able to displace receptors from signaling domains.Development of omega 3 fatty acid for therapeutic uses in fibrosis and tissue repairrequires a deeper understanding of additional intracellular signaling components,which is facilitated by a wider range of in vitro models.ObjectivesTo establish whether ERA, and its relative Docosahexaenoic acid (DMA), couldprevent cytokine-induced damage in models of atrophy and pathological fibrosis andidentify some of the mechanisms involved.MethodsUsing the cell line, C2C12, a well established model of myogenesis, ERA and DMAwere investi…
author: Whittingham-Dowd, JK
language: en
date published: 2012
date modified: 2026-07-24T04:26:12Z
provider: U. of Salford
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: U. of Salford