Host-Parasite Interrelations: Bacterial Adherence to and Invasion of Epithelial Cells
type: CreativeWork
description: Enteroinvasive Escherichia coli (EIEC) preferentially invaded multinucleated HeLa cells which arose naturally in monolayer cultures at a low frequency. Data suggests that enhancement of the penetration step accounts partly for increased invasion of multinucleated cells. Treatment of HeLa cell monolayers with polyethylene glycol (PEG) led to cell fusion, and invasion of PEG-treated monolayers by EIEC was increased by an average of 7-fold compared to untreated HeLa cells. Treatment of HeLa cells with PEG had no significant effect on the low level of cell-association displayed by noninvasive organisms and thus, PEG-treated HeLa cells appeared to retain their selectivity for EIEC. The microfilament disrupting agent cytochalasin B (CB) reduced EIEC invasion by more than 50-fold. Furthermore, CB depressed the extent of EIEC invasion of PEG-treated and untreated HeLa cells to equivalent levels. The large, multinucleated HeLa cells generated by PEG treatment revealed a striking accumulation of EIEC in cellular extensions. This study demonstrates that alteration of HeLa cell morphology results in increased invasion by EIEC. Colonial variants of Neisseria gonorrhoeae differ in their interactions with eucaryotic cells. Gonococci giving rise to the opaque colony type possess one or more proteins II in their outer membrane. When gonococci were cultivated with HeLa cell monolayers, the opaque phenotype became increasingly dominant in the subpopulation of organisms which adhered to the HeLa cells. Once bound, opaque organisms displayed very low levels of detachment. Adherent opaque gonococci exhibited generation times up to three-fold greater than cultures containing gonococci in the absence of HeLa cells. In addition, the progeny of adherent opaque organisms remained bound to the HeLa cell monolayer. Both piliated and transparent colony types attached to HeLa cells, but their progeny were retained less efficiently. Gonococci bound to HeLa cells were subjected to the bactericidal …
abstract: Enteroinvasive Escherichia coli (EIEC) preferentially invaded multinucleated HeLa cells which arose naturally in monolayer cultures at a low frequency. Data suggests that enhancement of the penetration step accounts partly for increased invasion of multinucleated cells. Treatment of HeLa cell monolayers with polyethylene glycol (PEG) led to cell fusion, and invasion of PEG-treated monolayers by EIEC was increased by an average of 7-fold compared to untreated HeLa cells. Treatment of HeLa cells with PEG had no significant effect on the low level of cell-association displayed by noninvasive organisms and thus, PEG-treated HeLa cells appeared to retain their selectivity for EIEC. The microfilament disrupting agent cytochalasin B (CB) reduced EIEC invasion by more than 50-fold. Furthermore, CB depressed the extent of EIEC invasion of PEG-treated and untreated HeLa cells to equivalent levels. The large, multinucleated HeLa cells generated by PEG treatment revealed a striking accumulation of EIEC in cellular extensions. This study demonstrates that alteration of HeLa cell morphology results in increased invasion by EIEC. Colonial variants of Neisseria gonorrhoeae differ in their interactions with eucaryotic cells. Gonococci giving rise to the opaque colony type possess one or more proteins II in their outer membrane. When gonococci were cultivated with HeLa cell monolayers, the opaque phenotype became increasingly dominant in the subpopulation of organisms which adhered to the HeLa cells. Once bound, opaque organisms displayed very low levels of detachment. Adherent opaque gonococci exhibited generation times up to three-fold greater than cultures containing gonococci in the absence of HeLa cells. In addition, the progeny of adherent opaque organisms remained bound to the HeLa cell monolayer. Both piliated and transparent colony types attached to HeLa cells, but their progeny were retained less efficiently. Gonococci bound to HeLa cells were subjected to the bactericidal …
author: Bessen, Debra
keywords: Enteroinvasive Escherichia coli (EIEC), multinucleated HeLa cells, Protein II (P.II), gonococcal adherence, cytochalasin B, tissue tropism, Life Sciences
date published: 1987-01-01T08:00:00Z
date modified: 2026-07-24T04:11:28Z
provider: Rockefeller
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Rockefeller