A Census of Human RNA-Binding Proteins and Characterization of the DEDDh RNA Exonuclease NEF-sp in the 3' End Maturation of 28S Ribosomal RNA
type: CreativeWork
description: Posttranscriptional gene regulation (PTGR) concerns all processes acting directly upon coding and non-coding RNAs, regulating and executing their maturation, ribonucleoprotein assembly, transport, stability and translation. RNA-binding proteins (RBPs) and ribonucleoprotein (RNP) complexes coordinate these processes. RBPs are central to cellular metabolism and their role in human diseases has been widely studied. Recent large-scale quantitative methods such as nextgeneration sequencing and modern protein mass spectrometry enabled new approaches to dissect PTGR networks and renewed interest in investigations of factors involved in PTGR at a genomewide level. A census of all coding and noncoding RBPs has previously not been readily available and the number of RBPs was estimated based on few selected protein classes. However, for system-wide analyses of PTGR a comprehensive account of the RBPs is necessary. To address this need, I developed a census of 1,542 manually curated RBPs and categorized their interactions with different classes of RNA, defined the number of factors in different regulatory pathways, and investigated their evolutionary patterns, abundance, and tissue-specific expression. Coregulated gene expression during developmental processes often gives novel insights into regulatory pathways and components. Furthermore, I showed that by classifying RBPs into their main regulatory RNA pathways we can start to understand the disease phenotypes of proteins involved in the same RNA metabolic pathways. These insights are useful for dissecting dysregulated PTGR pathways in human diseases and finding new therapeutic targets. Finally, I showed in this chapter that by careful domain analysis, novel RBPs can be predicted and characterized the previously unknown RG/GG-rich RBP FAM98A. Overall, this analysis provides a critical step towards the comprehensive characterization of proteins involved in human RNA metabolism. In the second part of the thesis I focused on the …
abstract: Posttranscriptional gene regulation (PTGR) concerns all processes acting directly upon coding and non-coding RNAs, regulating and executing their maturation, ribonucleoprotein assembly, transport, stability and translation. RNA-binding proteins (RBPs) and ribonucleoprotein (RNP) complexes coordinate these processes. RBPs are central to cellular metabolism and their role in human diseases has been widely studied. Recent large-scale quantitative methods such as nextgeneration sequencing and modern protein mass spectrometry enabled new approaches to dissect PTGR networks and renewed interest in investigations of factors involved in PTGR at a genomewide level. A census of all coding and noncoding RBPs has previously not been readily available and the number of RBPs was estimated based on few selected protein classes. However, for system-wide analyses of PTGR a comprehensive account of the RBPs is necessary. To address this need, I developed a census of 1,542 manually curated RBPs and categorized their interactions with different classes of RNA, defined the number of factors in different regulatory pathways, and investigated their evolutionary patterns, abundance, and tissue-specific expression. Coregulated gene expression during developmental processes often gives novel insights into regulatory pathways and components. Furthermore, I showed that by classifying RBPs into their main regulatory RNA pathways we can start to understand the disease phenotypes of proteins involved in the same RNA metabolic pathways. These insights are useful for dissecting dysregulated PTGR pathways in human diseases and finding new therapeutic targets. Finally, I showed in this chapter that by careful domain analysis, novel RBPs can be predicted and characterized the previously unknown RG/GG-rich RBP FAM98A. Overall, this analysis provides a critical step towards the comprehensive characterization of proteins involved in human RNA metabolism. In the second part of the thesis I focused on the …
author: Gerstberger, Stefanie
keywords: post-transcriptional regulation, RNA-binding proteins, ribonucleoprotein complexes, 28S rRNA maturation, NEF-sp exonuclease, tissue-specific expression, Life Sciences
date published: 2016-01-01T08:00:00Z
date modified: 2026-07-24T04:11:45Z
provider: Rockefeller
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Rockefeller