{"id":{"repo_id":"rice","oai_identifier":"oai:repository.rice.edu:1911/62198"},"canonical_url":"https://search.dev.ndltd.org/etd/rice/oai:repository.rice.edu:1911/62198","repository":{"repo_id":"rice","name":"Rice University","base_url":"https://repository.rice.edu/server/oai/request"},"display":{"title":"Nanoshells in vivo imaging using two-photon excitation microscopy","abstract":"This thesis describes the development and optical characterization of near infrared (NIR) gold nanoshells for the use as luminescent contrast agents for applications in small animal blood vessel imaging. Two types of gold-silica nanoshells excitable by NIR lasers are investigated: Type 1 nanoshells can be excited with a sub-mum NW laser, whereas Type 2 nanoshells can be excited with a NW laser in the micrometer range. Using NIR microscopy as an imaging platform, ex vivo and in vivo experiments are conducted to determine the efficacy of these nanoshells as suitable contrast agents. Specifically, individual particles of Type 1 nanoshells are successfully imaged and shown to provide bright optical contrast for blood vessel imaging both ex vivo and in vivo, while the Type 2 nanoshells are clearly imaged within the blood vessels ex vivo. These positive results suggest a promising possibility of developing a new class of contrast agents for deep tissue imaging and improving the imaging depth of NIR imaging techniques.","abstract_html":"This thesis describes the development and optical characterization of near infrared (NIR) gold nanoshells for the use as luminescent contrast agents for applications in small animal blood vessel imaging. Two types of gold-silica nanoshells excitable by NIR lasers are investigated: Type 1 nanoshells can be excited with a sub-mum NW laser, whereas Type 2 nanoshells can be excited with a NW laser in the micrometer range. Using NIR microscopy as an imaging platform, ex vivo and in vivo experiments are conducted to determine the efficacy of these nanoshells as suitable contrast agents. Specifically, individual particles of Type 1 nanoshells are successfully imaged and shown to provide bright optical contrast for blood vessel imaging both ex vivo and in vivo, while the Type 2 nanoshells are clearly imaged within the blood vessels ex vivo. These positive results suggest a promising possibility of developing a new class of contrast agents for deep tissue imaging and improving the imaging depth of NIR imaging techniques.","abstract_has_math":false,"creators":["Gao, Liang"],"institution":"Rice University","degree_name":"Master of Science","degree_level":"Masters","degree_discipline":"Engineering","degree_department":null,"school":null,"contributors":[],"advisors":["Dickinson, Mary E."],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010","date_published":"2010","updated_at":"2026-07-24T04:10:32Z","subjects":["Nanotechnology","Biochemistry","Physiology","Biophysics"],"languages":["eng"],"rights":["Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/1911/62198","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Dickinson, Mary E."]},{"key":"dc:creator","label":"Author","values":["Gao, Liang"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2011-07-25T02:07:25Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2011-07-25T02:07:25Z"]},{"key":"dc:date.issued","label":"Date","values":["2010"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Rice University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Nanotechnology","Biochemistry","Physiology","Biophysics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright is held by the author, unless otherwise indicated. 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Using NIR microscopy as an imaging platform, ex vivo and in vivo experiments are conducted to determine the efficacy of these nanoshells as suitable contrast agents. Specifically, individual particles of Type 1 nanoshells are successfully imaged and shown to provide bright optical contrast for blood vessel imaging both ex vivo and in vivo, while the Type 2 nanoshells are clearly imaged within the blood vessels ex vivo. These positive results suggest a promising possibility of developing a new class of contrast agents for deep tissue imaging and improving the imaging depth of NIR imaging techniques."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Nanoshells in vivo imaging using two-photon excitation microscopy"]}]}],"canonical_facts":{"dc:contributor.advisor":["Dickinson, Mary E."],"dc:creator":["Gao, Liang"],"dc:date.accessioned":["2011-07-25T02:07:25Z"],"dc:date.available":["2011-07-25T02:07:25Z"],"dc:date.issued":["2010"],"dc:description.abstract":["This thesis describes the development and optical characterization of near infrared (NIR) gold nanoshells for the use as luminescent contrast agents for applications in small animal blood vessel imaging. Two types of gold-silica nanoshells excitable by NIR lasers are investigated: Type 1 nanoshells can be excited with a sub-mum NW laser, whereas Type 2 nanoshells can be excited with a NW laser in the micrometer range. Using NIR microscopy as an imaging platform, ex vivo and in vivo experiments are conducted to determine the efficacy of these nanoshells as suitable contrast agents. Specifically, individual particles of Type 1 nanoshells are successfully imaged and shown to provide bright optical contrast for blood vessel imaging both ex vivo and in vivo, while the Type 2 nanoshells are clearly imaged within the blood vessels ex vivo. These positive results suggest a promising possibility of developing a new class of contrast agents for deep tissue imaging and improving the imaging depth of NIR imaging techniques."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["https://hdl.handle.net/1911/62198"],"dc:language.iso":["eng"],"dc:rights":["Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder."],"dc:subject":["Nanotechnology","Biochemistry","Physiology","Biophysics"],"dc:title":["Nanoshells in vivo imaging using two-photon excitation microscopy"],"dc:type":["Thesis"],"thesis:degree_discipline":["Engineering"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Rice University"]},"updated_at":"2026-07-24T04:10:32Z"}