{"id":{"repo_id":"rice","oai_identifier":"oai:repository.rice.edu:1911/62027"},"canonical_url":"https://search.dev.ndltd.org/etd/rice/oai:repository.rice.edu:1911/62027","repository":{"repo_id":"rice","name":"Rice University","base_url":"https://repository.rice.edu/server/oai/request"},"display":{"title":"Uncovering the signal transduction pathway that regulates the proliferation of Dictyostelium cells","abstract":"AprA (Autocrine Proliferation Repressor) is a secreted factor that represses proliferation in the slime mold Diclyostelium discoideum . Although the role AprA plays in the regulation of proliferation is known, the signaling pathway by which it occurs is yet to be determined. To discover the different components of the AprA signaling pathway, I examined 12 REMI mutants which proliferated in a similar manner to aprA - cells. Three of 12 mutants were insensitive to AprA. MetPOE was one of the mutants that were insensitive to AprA, and I attempted to construct a complete knockout of the disrupted gene. From an additional REMI screen that I conducted, I found that disruption of dokA led to a mutant with a phenotype similar to aprA-, suggesting this protein may also have a role in the AprA signal transduction pathway. Preliminary data suggested that G proteins may also play a role in the AprA signal transduction pathway. I further characterized six G protein null mutants. I determined that both galpha8- and gbeta- cells were multinucleate. Also there was an increase in the percentage of cells in S phase in gbeta - cells. The similarities between aprA- cells and ga8- and gbeta - cells suggested that Galpha8- and Gbeta - may be part of the AprA signal transduction pathway.","abstract_html":"AprA (Autocrine Proliferation Repressor) is a secreted factor that represses proliferation in the slime mold Diclyostelium discoideum . Although the role AprA plays in the regulation of proliferation is known, the signaling pathway by which it occurs is yet to be determined. To discover the different components of the AprA signaling pathway, I examined 12 REMI mutants which proliferated in a similar manner to aprA - cells. Three of 12 mutants were insensitive to AprA. MetPOE was one of the mutants that were insensitive to AprA, and I attempted to construct a complete knockout of the disrupted gene. From an additional REMI screen that I conducted, I found that disruption of dokA led to a mutant with a phenotype similar to aprA-, suggesting this protein may also have a role in the AprA signal transduction pathway. Preliminary data suggested that G proteins may also play a role in the AprA signal transduction pathway. I further characterized six G protein null mutants. I determined that both galpha8- and gbeta- cells were multinucleate. Also there was an increase in the percentage of cells in S phase in gbeta - cells. The similarities between aprA- cells and ga8- and gbeta - cells suggested that Galpha8- and Gbeta - may be part of the AprA signal transduction pathway.","abstract_has_math":false,"creators":["Hanson, Nana"],"institution":"Rice University","degree_name":"Master of Arts","degree_level":"Masters","degree_discipline":"Natural Sciences","degree_department":null,"school":null,"contributors":[],"advisors":["Gomer, Richard H."],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010","date_published":"2010","updated_at":"2026-07-24T04:10:36Z","subjects":["Cellular biology","Chemistry","Biochemistry"],"languages":["eng"],"rights":["Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/1911/62027","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Gomer, Richard H."]},{"key":"dc:creator","label":"Author","values":["Hanson, Nana"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2011-07-25T02:05:26Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2011-07-25T02:05:26Z"]},{"key":"dc:date.issued","label":"Date","values":["2010"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Natural Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Arts"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Rice University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Cellular biology","Chemistry","Biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/1911/62027"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["AprA (Autocrine Proliferation Repressor) is a secreted factor that represses proliferation in the slime mold Diclyostelium discoideum . Although the role AprA plays in the regulation of proliferation is known, the signaling pathway by which it occurs is yet to be determined. To discover the different components of the AprA signaling pathway, I examined 12 REMI mutants which proliferated in a similar manner to aprA - cells. Three of 12 mutants were insensitive to AprA. MetPOE was one of the mutants that were insensitive to AprA, and I attempted to construct a complete knockout of the disrupted gene. From an additional REMI screen that I conducted, I found that disruption of dokA led to a mutant with a phenotype similar to aprA-, suggesting this protein may also have a role in the AprA signal transduction pathway. Preliminary data suggested that G proteins may also play a role in the AprA signal transduction pathway. I further characterized six G protein null mutants. I determined that both galpha8- and gbeta- cells were multinucleate. Also there was an increase in the percentage of cells in S phase in gbeta - cells. The similarities between aprA- cells and ga8- and gbeta - cells suggested that Galpha8- and Gbeta - may be part of the AprA signal transduction pathway."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Uncovering the signal transduction pathway that regulates the proliferation of Dictyostelium cells"]}]}],"canonical_facts":{"dc:contributor.advisor":["Gomer, Richard H."],"dc:creator":["Hanson, Nana"],"dc:date.accessioned":["2011-07-25T02:05:26Z"],"dc:date.available":["2011-07-25T02:05:26Z"],"dc:date.issued":["2010"],"dc:description.abstract":["AprA (Autocrine Proliferation Repressor) is a secreted factor that represses proliferation in the slime mold Diclyostelium discoideum . Although the role AprA plays in the regulation of proliferation is known, the signaling pathway by which it occurs is yet to be determined. To discover the different components of the AprA signaling pathway, I examined 12 REMI mutants which proliferated in a similar manner to aprA - cells. Three of 12 mutants were insensitive to AprA. MetPOE was one of the mutants that were insensitive to AprA, and I attempted to construct a complete knockout of the disrupted gene. From an additional REMI screen that I conducted, I found that disruption of dokA led to a mutant with a phenotype similar to aprA-, suggesting this protein may also have a role in the AprA signal transduction pathway. Preliminary data suggested that G proteins may also play a role in the AprA signal transduction pathway. I further characterized six G protein null mutants. I determined that both galpha8- and gbeta- cells were multinucleate. Also there was an increase in the percentage of cells in S phase in gbeta - cells. The similarities between aprA- cells and ga8- and gbeta - cells suggested that Galpha8- and Gbeta - may be part of the AprA signal transduction pathway."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["https://hdl.handle.net/1911/62027"],"dc:language.iso":["eng"],"dc:rights":["Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder."],"dc:subject":["Cellular biology","Chemistry","Biochemistry"],"dc:title":["Uncovering the signal transduction pathway that regulates the proliferation of Dictyostelium cells"],"dc:type":["Thesis"],"thesis:degree_discipline":["Natural Sciences"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Arts"],"thesis:institution_name":["Rice University"]},"updated_at":"2026-07-24T04:10:36Z"}