{"id":{"repo_id":"rice","oai_identifier":"oai:repository.rice.edu:1911/17521"},"canonical_url":"https://search.dev.ndltd.org/etd/rice/oai:repository.rice.edu:1911/17521","repository":{"repo_id":"rice","name":"Rice University","base_url":"https://repository.rice.edu/server/oai/request"},"display":{"title":"Membrane tether formation from outer hair cells with optical tweezers","abstract":"The mechanical properties of the outer hair cells (OHCs) lateral wall are essential to elucidate the mechanism of OHC electromotility. Optical tweezers were used to characterize the mechanical properties of OHC plasma membrane (PM) by pulling tethers. A greater force was required for tether formations from OHC lateral wall (499 pN +/- 152) than from OHC basal end (142 pN +/- 49), consistent with the presence of a more extensive cytoskeleton support beneath the PM at the lateral wall. The apparent PM stiffness, which was estimated by measuring tether force at different tether length, was 3.71 pN/mum for OHC lateral wall and 4.57 pN/mum for OHC basal end. The apparent PM viscosity was measured by pulling tethers at different rates while continuously recording the tether force, and was estimated in the range of 13--33 pN·s/mum.","abstract_html":"The mechanical properties of the outer hair cells (OHCs) lateral wall are essential to elucidate the mechanism of OHC electromotility. Optical tweezers were used to characterize the mechanical properties of OHC plasma membrane (PM) by pulling tethers. A greater force was required for tether formations from OHC lateral wall (499 pN +/- 152) than from OHC basal end (142 pN +/- 49), consistent with the presence of a more extensive cytoskeleton support beneath the PM at the lateral wall. The apparent PM stiffness, which was estimated by measuring tether force at different tether length, was 3.71 pN/mum for OHC lateral wall and 4.57 pN/mum for OHC basal end. The apparent PM viscosity was measured by pulling tethers at different rates while continuously recording the tether force, and was estimated in the range of 13--33 pN·s/mum.","abstract_has_math":false,"creators":["Li, Zhiwei"],"institution":"Rice University","degree_name":"Master of Science","degree_level":"Masters","degree_discipline":"Engineering","degree_department":null,"school":null,"contributors":[],"advisors":["Brownell, William"],"committee_chairs":[],"committee_members":[],"year":2002,"date_issued":"2002","date_published":"2002","updated_at":"2026-07-24T04:10:19Z","subjects":["Biomedical engineering","Biophysics"],"languages":["eng"],"rights":["Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/1911/17521","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Brownell, William"]},{"key":"dc:creator","label":"Author","values":["Li, Zhiwei"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2009-06-04T06:42:22Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2009-06-04T06:42:22Z"]},{"key":"dc:date.issued","label":"Date","values":["2002"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Rice University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biomedical engineering","Biophysics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/1911/17521"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The mechanical properties of the outer hair cells (OHCs) lateral wall are essential to elucidate the mechanism of OHC electromotility. Optical tweezers were used to characterize the mechanical properties of OHC plasma membrane (PM) by pulling tethers. A greater force was required for tether formations from OHC lateral wall (499 pN +/- 152) than from OHC basal end (142 pN +/- 49), consistent with the presence of a more extensive cytoskeleton support beneath the PM at the lateral wall. The apparent PM stiffness, which was estimated by measuring tether force at different tether length, was 3.71 pN/mum for OHC lateral wall and 4.57 pN/mum for OHC basal end. The apparent PM viscosity was measured by pulling tethers at different rates while continuously recording the tether force, and was estimated in the range of 13--33 pN·s/mum."]},{"key":"dc:format.mimetype","label":"Dc Format Mimetype","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Membrane tether formation from outer hair cells with optical tweezers"]}]}],"canonical_facts":{"dc:contributor.advisor":["Brownell, William"],"dc:creator":["Li, Zhiwei"],"dc:date.accessioned":["2009-06-04T06:42:22Z"],"dc:date.available":["2009-06-04T06:42:22Z"],"dc:date.issued":["2002"],"dc:description.abstract":["The mechanical properties of the outer hair cells (OHCs) lateral wall are essential to elucidate the mechanism of OHC electromotility. Optical tweezers were used to characterize the mechanical properties of OHC plasma membrane (PM) by pulling tethers. A greater force was required for tether formations from OHC lateral wall (499 pN +/- 152) than from OHC basal end (142 pN +/- 49), consistent with the presence of a more extensive cytoskeleton support beneath the PM at the lateral wall. The apparent PM stiffness, which was estimated by measuring tether force at different tether length, was 3.71 pN/mum for OHC lateral wall and 4.57 pN/mum for OHC basal end. The apparent PM viscosity was measured by pulling tethers at different rates while continuously recording the tether force, and was estimated in the range of 13--33 pN·s/mum."],"dc:format.mimetype":["application/pdf"],"dc:identifier.uri":["https://hdl.handle.net/1911/17521"],"dc:language.iso":["eng"],"dc:rights":["Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder."],"dc:subject":["Biomedical engineering","Biophysics"],"dc:title":["Membrane tether formation from outer hair cells with optical tweezers"],"dc:type":["Thesis"],"thesis:degree_discipline":["Engineering"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Rice University"]},"updated_at":"2026-07-24T04:10:19Z"}