PHYSICAL MAPPING OF THE ORIGINS OF REPLICATION OF BACILLUS SUBTILIS BACTERIOPHAGE SP01

The DNA of Bacillus subtilis bacteriophage SP01 is a linear duplex 140 kilobasepairs long, with direct terminal repeats of 12.4 kilobasepairs. It contains the unusual base 5-hydroxymethyluracil in place of thymine. Southern transfer hybridization analysis of pulse-labeled early replicating intermediates was used to localize the origins of replication on the physical map of SP01. The fragments that were consistently detected in this manner correspond to a region of the DNA extending from the middle of the left terminal repeat to about 32 kilobasepairs from the left end of the genome. Thus there is at least one origin of replication between the middle of the left terminal repeat and a point about 32 kilobasepairs from the left end of the genome. However, because of the nature of the terminal repeats and the ambiguity of the restriction maps of this region, as many as three origins of replication may be indicated: one in each direct terminal repeat and one about 30-32 kilobasepairs from the left end of the genome, in the vicinity of Xba I fragment 16b.

Name thesis:degree_name

Doctor of Philosophy

Level thesis:degree_level

Doctoral

Discipline thesis:degree_discipline

Natural Sciences

Grantor

Rice University

Year dc:date.issued

1985

Author dc:creator

• DOLBY, NICHOL

• Microbiology

Statement dc:rights

• Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1911/15894

OAI identifier oai:identifier

oai:repository.rice.edu:1911/15894