The influence of culture environment on antigen shedding from the surface of colon cancer cells

The roles of some nutrients and environmental factors on CEA synthesis and release has been determined for the LS180 colon cancer cell line. When these cells were grown on various carbohydrate sources, cells grown on mannose exhibited the highest release rates at 6.5 ng CEA/mg protein/hour. When 25mM glucose with 10% FBS was used, the release rates were 3.8 ng CEA/mg protein/hour, the lowest of any of the cultures. The effects of nutrient depletion revealed that replacing the media at 145 hours after inoculation inhibited both the rate of synthesis and release of CEA. Feeding the cells at 100 hours eliminated antigen synthesis and release. Elevating incubation temperatures from 37$\sp\circ$C for 30 minutes at 100 hours increased the rates of both CEA synthesis and release from near zero to 150 ng CEA/mg protein/hour for cells heated to 45$\sp\circ$C and 42$\sp\circ$C. (Abstract shortened with permission of author.)

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Engineering

Grantor

Rice University

Year dc:date.issued

1989

Author dc:creator

• Adams, Gretchen Louise

Advisor dc:contributor.advisor

• McIntire, Larry V.

• Chemical engineering
• Pathology

Statement dc:rights

• Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1911/13336

OAI identifier oai:identifier

oai:repository.rice.edu:1911/13336