Single-Molecule and Ensemble Measurements Inform on the Role of Protein Crowding at Interfaces
type: CreativeWork
description: Surface-adsorbed proteins can critically affect the efficacy of materials designed for biological applications. For example, physiologically relevant quantities of serum albumin stabilize nanoparticles in solution, while decreased protein concentrations induce nanoparticle aggregation. The first aim of this dissertation was to characterize the crowding mechanism of the most abundant serum protein, serum albumin. Many studies have explored serum protein conformation on albumin-crowded surfaces, but unanswered questions about the mechanism of interaction between different proteins remain. Single-molecule high-resolution imaging with photobleaching (SHRImP) was applied to measure dye-conjugated fibronectin’s unfolding in varying conditions of crowding with serum albumin on aminosilanized glass. Our results indicated that serum albumin sterically restricts fibronectin’s unfolding during the adsorption process. Because fibronectin’s conformation is dependent on interfacial macromolecular crowding under in vitro conditions, it is important to consider the role of in vivo crowding on protein activity. The second aim of this dissertation was to characterize the chiral aggregation of different protein-nanoparticle aggregates as a function of crowding and to gain insight into the protein-nanoparticle aggregation mechanism. Serum albumin induces gold nanorods to aggregate and produces protein-nanorod aggregates that exhibit circular dichroism. These aggregates could be applicable to sensitive chirality detection, but the mechanisms by which they form and produce circular dichroism are not well understood. Circular dichroism spectroscopy and scanning electron microscopy were implemented to characterize the nanorod aggregates produced with globular and fibrous proteins over varied protein/nanorod ratios. Serum albumin and other globular proteins produced similar chiral protein-nanorod aggregates that were distinct from the aggregates produced by fibrous proteins. Further, CTAB, …
abstract: Surface-adsorbed proteins can critically affect the efficacy of materials designed for biological applications. For example, physiologically relevant quantities of serum albumin stabilize nanoparticles in solution, while decreased protein concentrations induce nanoparticle aggregation. The first aim of this dissertation was to characterize the crowding mechanism of the most abundant serum protein, serum albumin. Many studies have explored serum protein conformation on albumin-crowded surfaces, but unanswered questions about the mechanism of interaction between different proteins remain. Single-molecule high-resolution imaging with photobleaching (SHRImP) was applied to measure dye-conjugated fibronectin’s unfolding in varying conditions of crowding with serum albumin on aminosilanized glass. Our results indicated that serum albumin sterically restricts fibronectin’s unfolding during the adsorption process. Because fibronectin’s conformation is dependent on interfacial macromolecular crowding under in vitro conditions, it is important to consider the role of in vivo crowding on protein activity. The second aim of this dissertation was to characterize the chiral aggregation of different protein-nanoparticle aggregates as a function of crowding and to gain insight into the protein-nanoparticle aggregation mechanism. Serum albumin induces gold nanorods to aggregate and produces protein-nanorod aggregates that exhibit circular dichroism. These aggregates could be applicable to sensitive chirality detection, but the mechanisms by which they form and produce circular dichroism are not well understood. Circular dichroism spectroscopy and scanning electron microscopy were implemented to characterize the nanorod aggregates produced with globular and fibrous proteins over varied protein/nanorod ratios. Serum albumin and other globular proteins produced similar chiral protein-nanorod aggregates that were distinct from the aggregates produced by fibrous proteins. Further, CTAB, …
author: Warning, Lauren Amanda
language: eng
keywords: single-molecule microscopy, chiroptical sensing, protein corona
date published: 2021-08-13
date modified: 2026-07-24T04:10:36Z
provider: Rice University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Rice University

PropertyValue
property ID: Handle
value: https://hdl.handle.net/1911/111224