{"id":{"repo_id":"rice","oai_identifier":"oai:repository.rice.edu:1911/103542"},"canonical_url":"https://search.dev.ndltd.org/etd/rice/oai:repository.rice.edu:1911/103542","repository":{"repo_id":"rice","name":"Rice University","base_url":"https://repository.rice.edu/server/oai/request"},"display":{"title":"Endothelial cell and mural progenitor cell co-culture to promote angiogenesis in tissue engineered constructs","abstract":"The need for tissue engineered organs is great since only 15% of those on the transplant waiting list received organs in 2006. The ability to vascularize a tissue engineered construct is one of the major hurdles for tissue engineering because it can take 2 weeks for an implanted construct to become vascularized. To produce prevascularized tissues, the cellular interactions that occur during vascular formation need to be examined. In this work, human umbilical cord endothelial cells (HUVECs) and mural progenitor cells (10T1/2 cells) are cultured together for 6 days. Western blotting and immunofluorescence are performed to assess SM-specific marker expression. αSM-actin and calponin expression was observed in co-cultures seeded at 300,000 cells/well while caldesmon and SMMHC expression was observed in co-cultures seeded at 60,000 cells/well. HUVECs and 10T1/2 cells, seeded at 150,000 cells/gel, encapsulated in degradable PEG hydrogels expressed high levels of αSM-actin, calponin, and caldesmon.","abstract_html":"The need for tissue engineered organs is great since only 15% of those on the transplant waiting list received organs in 2006. The ability to vascularize a tissue engineered construct is one of the major hurdles for tissue engineering because it can take 2 weeks for an implanted construct to become vascularized. To produce prevascularized tissues, the cellular interactions that occur during vascular formation need to be examined. In this work, human umbilical cord endothelial cells (HUVECs) and mural progenitor cells (10T1/2 cells) are cultured together for 6 days. Western blotting and immunofluorescence are performed to assess SM-specific marker expression. αSM-actin and calponin expression was observed in co-cultures seeded at 300,000 cells/well while caldesmon and SMMHC expression was observed in co-cultures seeded at 60,000 cells/well. HUVECs and 10T1/2 cells, seeded at 150,000 cells/gel, encapsulated in degradable PEG hydrogels expressed high levels of αSM-actin, calponin, and caldesmon.","abstract_has_math":false,"creators":["Smith, April Ann"],"institution":"Rice University","degree_name":"Master of Science","degree_level":"Masters","degree_discipline":"Engineering","degree_department":null,"school":null,"contributors":[],"advisors":["West, Jennifer L."],"committee_chairs":[],"committee_members":[],"year":2008,"date_issued":"2008","date_published":"2008","updated_at":"2026-07-24T04:10:32Z","subjects":["Biomedical engineering","Applied sciences"],"languages":["eng"],"rights":["Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/1911/103542","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["West, Jennifer L."]},{"key":"dc:creator","label":"Author","values":["Smith, April Ann"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2018-12-03T18:31:17Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2018-12-03T18:31:17Z"]},{"key":"dc:date.issued","label":"Date","values":["2008"]},{"key":"dc:type","label":"Dc Type","values":["Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Rice University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biomedical engineering","Applied sciences"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/1911/103542"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The need for tissue engineered organs is great since only 15% of those on the transplant waiting list received organs in 2006. The ability to vascularize a tissue engineered construct is one of the major hurdles for tissue engineering because it can take 2 weeks for an implanted construct to become vascularized. To produce prevascularized tissues, the cellular interactions that occur during vascular formation need to be examined. In this work, human umbilical cord endothelial cells (HUVECs) and mural progenitor cells (10T1/2 cells) are cultured together for 6 days. Western blotting and immunofluorescence are performed to assess SM-specific marker expression. αSM-actin and calponin expression was observed in co-cultures seeded at 300,000 cells/well while caldesmon and SMMHC expression was observed in co-cultures seeded at 60,000 cells/well. HUVECs and 10T1/2 cells, seeded at 150,000 cells/gel, encapsulated in degradable PEG hydrogels expressed high levels of αSM-actin, calponin, and caldesmon."]},{"key":"dc:title","label":"Title","values":["Endothelial cell and mural progenitor cell co-culture to promote angiogenesis in tissue engineered constructs"]}]}],"canonical_facts":{"dc:contributor.advisor":["West, Jennifer L."],"dc:creator":["Smith, April Ann"],"dc:date.accessioned":["2018-12-03T18:31:17Z"],"dc:date.available":["2018-12-03T18:31:17Z"],"dc:date.issued":["2008"],"dc:description.abstract":["The need for tissue engineered organs is great since only 15% of those on the transplant waiting list received organs in 2006. The ability to vascularize a tissue engineered construct is one of the major hurdles for tissue engineering because it can take 2 weeks for an implanted construct to become vascularized. To produce prevascularized tissues, the cellular interactions that occur during vascular formation need to be examined. In this work, human umbilical cord endothelial cells (HUVECs) and mural progenitor cells (10T1/2 cells) are cultured together for 6 days. Western blotting and immunofluorescence are performed to assess SM-specific marker expression. αSM-actin and calponin expression was observed in co-cultures seeded at 300,000 cells/well while caldesmon and SMMHC expression was observed in co-cultures seeded at 60,000 cells/well. HUVECs and 10T1/2 cells, seeded at 150,000 cells/gel, encapsulated in degradable PEG hydrogels expressed high levels of αSM-actin, calponin, and caldesmon."],"dc:identifier.uri":["https://hdl.handle.net/1911/103542"],"dc:language.iso":["eng"],"dc:rights":["Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder."],"dc:subject":["Biomedical engineering","Applied sciences"],"dc:title":["Endothelial cell and mural progenitor cell co-culture to promote angiogenesis in tissue engineered constructs"],"dc:type":["Thesis"],"thesis:degree_discipline":["Engineering"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science"],"thesis:institution_name":["Rice University"]},"updated_at":"2026-07-24T04:10:32Z"}