Overcoming the seasonal variations in fitness of the amphipod Corophium Volutator as an environmental toxicology test species using laboratory cultured specimens.

The marine amphipod Corophium volutator is an important source of food for many fish and wading birds, making it an important species in an estuarine environment and a relevant toxicological test species. Historical data has indicated that seasonal variations in the fitness of C. volutator may make toxicity testing impossible during summer months. C. volutator were cultured in the laboratory to determine if variations in the fitness of wild C. volutator could be overcome with the use of cultured specimens. Adult and neonate C. volutator were cultured separately under different feeding regimes with Tetraselmis chuii and Rhodomonas reticulate as food sources. Cultures were maintained at a salinity of 30-35 ppt, with a temperature of 15 °C ± 2, dissolved oxygen was maintained at greater-than-or-equal-to 80% saturation. Assessments of growth rates, time until sexual maturity, and numbers of offspring produced were made from these trials. During this study, a growth rate of 0.0648 mm ± 0.0185 mm per day was determined. The preferred feeding regime was a combination of T. chuii and R. reticulate. The average number of offspring produced per C. volutator over 132 days of culturing was 8.6. Experiments indicate that C. volutator may be cultured under laboratory conditions; however, further work is required to increase the reproductive output of cultured C. volutator.

Name dc:type.qualificationname

MRes

Level dc:type.qualificationlevel

Masters

Grantor dc:publisher.institution

Robert Gordon University

Year dc:date.issued

2014

Author dc:creator

• McGee, Donall

Advisor dc:contributor.advisor

• Linda A. Lawton and Christine Edwards

• Corophium volutator
• Laboratory culture
• Reproduction
• Seasonal variation
• Toxicity testing
• Growth rate

Language dc:language

en

Identifier

oai:rgu-repository.worktribe.com:248591

OAI identifier oai:identifier

oai:rgu-repository.worktribe.com:248591