{"id":{"repo_id":"radboud","oai_identifier":"oai:repository.ubn.ru.nl:2066/19390"},"canonical_url":"https://search.dev.ndltd.org/etd/radboud/oai:repository.ubn.ru.nl:2066/19390","repository":{"repo_id":"radboud","name":"Radboud University Nijmegen","base_url":"https://repository.ubn.ru.nl/oai/request"},"display":{"title":"Role of the Tcell genotype in the development of allogeneic responses after stem cell transplantation and cellular immunotherapy.","abstract":"Contains fulltext : 19390.pdf (Publisher’s version ) (Open Access)","abstract_html":"Contains fulltext : 19390.pdf (Publisher’s version ) (Open Access)","abstract_has_math":false,"creators":["Kolen, S.M.H."],"institution":"S.l. : s.n.","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Witte, T.J.M. de","Wiel-van Kemenade, E. van de","Dolstra, H."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2004,"date_issued":"2004","date_published":"2004","updated_at":"2026-07-24T04:01:29Z","subjects":["UMCN 1.4: Immunotherapy, gene therapy and transplantation"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2066/58735","9090175571"],"render_values":[{"text":"http://hdl.handle.net/2066/58735","href":"http://hdl.handle.net/2066/58735","code":true},{"text":"9090175571","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2066/19390","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Witte, T.J.M. de","Wiel-van Kemenade, E. van de","Dolstra, H."]},{"key":"dc:creator","label":"Author","values":["Kolen, S.M.H."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2004"]},{"key":"dc:publisher","label":"Institution","values":["S.l. : s.n."]},{"key":"dc:type","label":"Dc Type","values":["Doctoral thesis"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["UMCN 1.4: Immunotherapy, gene therapy and transplantation"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://repository.ubn.ru.nl//bitstream/handle/2066/19390/19390.pdf","http://hdl.handle.net/2066/19390","http://hdl.handle.net/2066/58735","9090175571"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Contains fulltext : 19390.pdf (Publisher’s version ) (Open Access)","Allogeneic hematopoietic stem cell transplantation (SCT) following high-dose systemic chemo- or chemoradiotherapy is the most effective curative treatment for patients with leukemia. Donor graft-derived T cells can mediate an immunological elimination of residual leukemic cells. Relapse of leukemia after allogeneic SCT can be successfully treated with donor lymphocyte infusions (DLI). The genotype of the bone marrow, or, the extent of hematopoietic chimerism, at the time of relapse, may influence the inititation and magnitude of T cell mediated graft-versus-malignancy reactivity. The mechanisms by which either donor-derived or recipient-derived T cells contribute to tolerance or non-responsiveness of infused donor lymphocytes have not been clearly demonstrated yet. The research described in this thesis focusses on the role of the T cell genotype in the development of alloreactive immune responses. The first part of the thesis describes the development of a methodology to study the fate of infused T cells, and its implementation in in vivo mouse and rat transplantation models. In Chapter 2 we showed that the period of in vitro stimulation and expansion of syngeneic murine splenic T cells before infusion strongly influences their survival and trafficking patterns. Using an optimized culture and gene transduction protocol we studied the capacity of T cells to evoke allogeneic responses in a rat model after BMT, supplemented with retrovirally transduced T cells (Chapter 3). In Chapter 4 we identified a mechanism that may contribute to unresponsiveness to DLI in relapsed patients. Recurrent T cells of recipient origin, present at the time of DLI, may inhibit alloreactivity of infused donor lymphocytes by eliminating these cells. In the final part of this thesis we described the development of a method to quantitatively monitor the genetic origin of hematopoietic cells (Chapter 5), and used this technique in a clinical study to monitor changes in chimerism in relation to altered alloimmune reactivity (Chapter 6)","KUN, 22 januari 2004","Promotor : Witte, T.J.M. de Co-promotores : Wiel-van Kemenade, E. van de, Dolstra, H.","150 p."]},{"key":"dc:title","label":"Title","values":["Role of the Tcell genotype in the development of allogeneic responses after stem cell transplantation and cellular immunotherapy."]}]}],"canonical_facts":{"dc:contributor":["Witte, T.J.M. de","Wiel-van Kemenade, E. van de","Dolstra, H."],"dc:creator":["Kolen, S.M.H."],"dc:date":["2004"],"dc:description":["Contains fulltext : 19390.pdf (Publisher’s version ) (Open Access)","Allogeneic hematopoietic stem cell transplantation (SCT) following high-dose systemic chemo- or chemoradiotherapy is the most effective curative treatment for patients with leukemia. Donor graft-derived T cells can mediate an immunological elimination of residual leukemic cells. Relapse of leukemia after allogeneic SCT can be successfully treated with donor lymphocyte infusions (DLI). The genotype of the bone marrow, or, the extent of hematopoietic chimerism, at the time of relapse, may influence the inititation and magnitude of T cell mediated graft-versus-malignancy reactivity. The mechanisms by which either donor-derived or recipient-derived T cells contribute to tolerance or non-responsiveness of infused donor lymphocytes have not been clearly demonstrated yet. The research described in this thesis focusses on the role of the T cell genotype in the development of alloreactive immune responses. The first part of the thesis describes the development of a methodology to study the fate of infused T cells, and its implementation in in vivo mouse and rat transplantation models. In Chapter 2 we showed that the period of in vitro stimulation and expansion of syngeneic murine splenic T cells before infusion strongly influences their survival and trafficking patterns. Using an optimized culture and gene transduction protocol we studied the capacity of T cells to evoke allogeneic responses in a rat model after BMT, supplemented with retrovirally transduced T cells (Chapter 3). In Chapter 4 we identified a mechanism that may contribute to unresponsiveness to DLI in relapsed patients. Recurrent T cells of recipient origin, present at the time of DLI, may inhibit alloreactivity of infused donor lymphocytes by eliminating these cells. In the final part of this thesis we described the development of a method to quantitatively monitor the genetic origin of hematopoietic cells (Chapter 5), and used this technique in a clinical study to monitor changes in chimerism in relation to altered alloimmune reactivity (Chapter 6)","KUN, 22 januari 2004","Promotor : Witte, T.J.M. de Co-promotores : Wiel-van Kemenade, E. van de, Dolstra, H.","150 p."],"dc:identifier":["https://repository.ubn.ru.nl//bitstream/handle/2066/19390/19390.pdf","http://hdl.handle.net/2066/19390","http://hdl.handle.net/2066/58735","9090175571"],"dc:publisher":["S.l. : s.n."],"dc:subject":["UMCN 1.4: Immunotherapy, gene therapy and transplantation"],"dc:title":["Role of the Tcell genotype in the development of allogeneic responses after stem cell transplantation and cellular immunotherapy."],"dc:type":["Doctoral thesis"]},"updated_at":"2026-07-24T04:01:29Z"}